Int J Biol Macromol 2025 Feb 06;304Pt 1:140721. doi: 10.1016/j.ijbiomac.2025.140721.

Investigation on the mechanism of anionic polysaccharides affecting the gastrointestinal digestion fate of sea cucumber collagen fibrils.

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Previous research has demonstrated that fucosylated chondroitin sulfate (fCS) and fucoidan (FUC) enhance the gastrointestinal digestion of sea cucumber collagen fibrils, whereas kappa-carrageenan (K-car) and sodium alginate (SA) exhibit inhibitory effects. The objective of this study was to investigate the mechanisms by which these anionic polysaccharides modulate collagen fibril digestion. Upon the addition of fCS and FUC, the total hydroxyproline content in the insoluble precipitate was significantly reduced by 73.98% and 63.62%, respectively, after 2 h of gastric digestion. All four anionic polysaccharides were found to interact with the soluble digestion products of collagen fibrils, leading to fluorescence quenching. Notably, fCS and FUC displayed significantly stronger interactions with the digestion products compared to K-car and SA, as evidenced by fluorescence spectroscopy. Furthermore, heat treatment resulted in enhanced adsorption of polysaccharides onto the insoluble digestion products of collagen fibrils. Interestingly, the inclusion of polysaccharides, particularly fCS and FUC, substantially increased the turbidity of intestinal digestion products. These interactions were mediated by specific collagen, such as APA22677.1, PIK60696.1, PIK60691.1, PIK60692.1, PIK60693.1, and AYL88761.1 (NCBI). These findings provide crucial insights into how anionic polysaccharides influence the digestive behavior of collagen fibrils, offering potential applications in food science and nutrition.

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