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Front Chem
2023 Jan 01;11:1332921. doi: 10.3389/fchem.2023.1332921.
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Antioxidant and anticholinesterase properties of Echinometra mathaei and Ophiocoma erinaceus venoms from the Persian Gulf.
Dehghani H
,
Rashedinia M
,
Mohebbi G
,
Vazirizadeh A
,
Baghban N
.
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Introduction: The Persian Gulf is home to a diverse range of marine life, including various species of fish, crustaceans, mollusks, and echinoderms. This study investigates the potential therapeutic properties of venoms from echinoderms in the Persian Gulf, specifically their ability to inhibit cholinesterases (Acetylcholinesterase and butyrylcholinesterase) and act as antioxidants. Methods: Four venoms from two echinoderm species, including the spine, gonad, and coelomic fluids of sea urchins, as well as brittle star venoms, were analyzed using various methods, including LD50 determination, protein analysis, antioxidant assays, GC-MS for secondary metabolite identification, and molecular docking simulations. Results and discussion: The study's results revealed the LD50 of the samples as follows: 2.231 ± 0.09, 1.03 ± 0.05, 1.12 ± 0.13, and 6.04 ± 0.13 mg/mL, respectively. Additionally, the protein levels were 44.037 ± 0.002, 74.223 ± 0.025, 469.97 ± 0.02, and 104.407 ± 0.025 μg/mL, respectively. SDS-PAGE and total protein studies indicated that at least part of the venom was proteinaceous. Furthermore, the study found that the brittle star samples exhibited significantly higher antioxidant activity compared to other samples, including the standard ascorbic acid, at all tested concentrations. GC-MS analysis identified 12, 23, 21, and 25 compounds in the samples, respectively. These compounds had distinct chemical and bioactive structures, including alkaloids, terpenes, and steroids. Conclusion: These venoms displayed strong cholinesterase inhibitory and antioxidant activities, likely attributed to their protein content and the presence of alkaloids, terpenes, and steroids. Notably, the alkaloid compound C 7 was identified as a promising candidate for further research in Alzheimer's disease therapy. In conclusion, echinoderms in the Persian Gulf may hold significant potential for discovering novel therapeutic agents.
Ageenko,
Pigment cell differentiation in sea urchin blastula-derived primary cell cultures.
2014,
Pubmed
,
Echinobase
Amini,
Metastatic Inhibitory and Radical Scavenging Efficacies of Saponins Extracted from the Brittle Star (Ophiocoma erinaceus).
2015,
Pubmed
,
Echinobase
Bayat,
Algal Cells-Derived Extracellular Vesicles: A Review With Special Emphasis on Their Antimicrobial Effects.
2021,
Pubmed
Brinkman,
Partial purification of cytolytic venom proteins from the box jellyfish, Chironex fleckeri.
2008,
Pubmed
Darabi,
Studies on the cholinesterases inhibiting compounds from the Cassiopea andromeda venom.
2020,
Pubmed
Dyshlovoy,
Inspired by Sea Urchins: Warburg Effect Mediated Selectivity of Novel Synthetic Non-Glycoside 1,4-Naphthoquinone-6S-Glucose Conjugates in Prostate Cancer.
2020,
Pubmed
,
Echinobase
George,
One-pot green synthesis of silver nanoparticles using brittle star Ophiocoma scolopendrina: Assessing biological potentialities of antibacterial, antioxidant, anti-diabetic and catalytic degradation of organic dyes.
2023,
Pubmed
,
Echinobase
Hamed,
Cholinesterase Inhibitors from an Endophytic Fungus Aspergillus niveus Fv-er401: Metabolomics, Isolation and Molecular Docking.
2023,
Pubmed
He,
Effects of harmine, an acetylcholinesterase inhibitor, on spatial learning and memory of APP/PS1 transgenic mice and scopolamine-induced memory impairment mice.
2015,
Pubmed
Kalinin,
Echinoderms Metabolites: Structure, Functions, and Biomedical Perspectives.
2021,
Pubmed
Klimenko,
Shallow- and Deep-Water Ophiura Species Produce a Panel of Chlorin Compounds with Potent Photodynamic Anticancer Activities.
2023,
Pubmed
,
Echinobase
Konrath,
Alkaloids as a source of potential anticholinesterase inhibitors for the treatment of Alzheimer's disease.
2013,
Pubmed
Kotzaeroglou,
The Role of Equilibrium between Free Radicals and Antioxidants in Depression and Bipolar Disorder.
2022,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Levina,
[Steroid compounds from the Far East starfish Pteraster obscurus and the ophiura Asteronyx loveni].
2007,
Pubmed
,
Echinobase
Maryamabadi,
Application of PEG-400 as a green biodegradable polymeric medium for the catalyst-free synthesis of spiro-dihydropyridines and their use as acetyl and butyrylcholinesterase inhibitors.
2016,
Pubmed
Mishchenko,
Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1.
2020,
Pubmed
,
Echinobase
Nuzzo,
Isolation of Chamigrene Sesquiterpenes and Absolute Configuration of Isoobtusadiene from the Brittle Star Ophionereis reticulata.
2017,
Pubmed
,
Echinobase
Polonik,
Synthesis, Cytotoxic Activity Evaluation and Quantitative Structure-Activity Analysis of Substituted 5,8-Dihydroxy-1,4-Naphthoquinones and their O- and S-Glycoside Derivatives Tested Against Neuro-2a Cancer Cells.
2020,
Pubmed
,
Echinobase
Rahman,
Prospects of Marine Sterols against Pathobiology of Alzheimer's Disease: Pharmacological Insights and Technological Advances.
2021,
Pubmed
Schardl,
Ergot alkaloids--biology and molecular biology.
2006,
Pubmed
Worek,
Improved determination of acetylcholinesterase activity in human whole blood.
1999,
Pubmed
Zare,
Chemical Compositions and Experimental and Computational Modeling of the Anticancer Effects of Cnidocyte Venoms of Jellyfish Cassiopea andromeda and Catostylus mosaicus on Human Adenocarcinoma A549 Cells.
2023,
Pubmed