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Lab Chip
2021 Jan 21;212:331-343. doi: 10.1039/d0lc00678e.
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A digital protein microarray for COVID-19 cytokine storm monitoring.
Song Y
,
Ye Y
,
Su SH
,
Stephens A
,
Cai T
,
Chung MT
,
Han MK
,
Newstead MW
,
Yessayan L
,
Frame D
,
Humes HD
,
Singer BH
,
Kurabayashi K
.
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Despite widespread concern regarding cytokine storms leading to severe morbidity in COVID-19, rapid cytokine assays are not routinely available for monitoring critically ill patients. We report the clinical application of a digital protein microarray platform for rapid multiplex quantification of cytokines from critically ill COVID-19 patients admitted to the intensive care unit (ICU) at the University of Michigan Hospital. The platform comprises two low-cost modules: (i) a semi-automated fluidic dispensing/mixing module that can be operated inside a biosafety cabinet to minimize the exposure of the technician to the virus infection and (ii) a 12-12-15 inch compact fluorescence optical scanner for the potential near-bedside readout. The platform enabled daily cytokine analysis in clinical practice with high sensitivity (<0.4 pg mL-1), inter-assay repeatability (∼10% CV), and rapid operation providing feedback on the progress of therapy within 4 hours. This test allowed us to perform serial monitoring of two critically ill patients with respiratory failure and to support immunomodulatory therapy using the selective cytopheretic device (SCD). We also observed clear interleukin-6 (IL-6) elevations after receiving tocilizumab (IL-6 inhibitor) while significant cytokine profile variability exists across all critically ill COVID-19 patients and to discover a weak correlation between IL-6 to clinical biomarkers, such as ferritin and C-reactive protein (CRP). Our data revealed large subject-to-subject variability in patients' response to COVID-19, reaffirming the need for a personalized strategy guided by rapid cytokine assays.
Akama,
Wash- and Amplification-Free Digital Immunoassay Based on Single-Particle Motion Analysis.
2019, Pubmed
Akama,
Wash- and Amplification-Free Digital Immunoassay Based on Single-Particle Motion Analysis.
2019,
Pubmed
Alcamo,
Role of Damage-Associated Molecular Patterns and Uncontrolled Inflammation in Pediatric Sepsis-Induced Multiple Organ Dysfunction Syndrome.
2019,
Pubmed
Araci,
A reusable microfluidic device provides continuous measurement capability and improves the detection limit of digital biology.
2016,
Pubmed
Chen,
Measuring IL-6 and sIL-6R in serum from patients treated with tocilizumab and/or siltuximab following CAR T cell therapy.
2016,
Pubmed
Chen,
Label-free cytokine micro- and nano-biosensing towards personalized medicine of systemic inflammatory disorders.
2015,
Pubmed
Coronaviridae Study Group of the International Committee on Taxonomy of Viruses,
The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2.
2020,
Pubmed
Del Valle,
An inflammatory cytokine signature predicts COVID-19 severity and survival.
2020,
Pubmed
Fan,
Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood.
2008,
Pubmed
Giamarellos-Bourboulis,
Complex Immune Dysregulation in COVID-19 Patients with Severe Respiratory Failure.
2020,
Pubmed
Griffin,
Hemophagocytic lymphohistiocytosis: An update on pathogenesis, diagnosis, and therapy.
2020,
Pubmed
Hadjadj,
Impaired type I interferon activity and inflammatory responses in severe COVID-19 patients.
2020,
Pubmed
Hoiland,
Amelioration of COVID-19-related cytokine storm syndrome: parallels to chimeric antigen receptor-T cell cytokine release syndrome.
2020,
Pubmed
Huang,
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China.
2020,
Pubmed
Kotch,
Tocilizumab for the treatment of chimeric antigen receptor T cell-induced cytokine release syndrome.
2019,
Pubmed
Kox,
Cytokine Levels in Critically Ill Patients With COVID-19 and Other Conditions.
2020,
Pubmed
Liu,
Longitudinal characteristics of lymphocyte responses and cytokine profiles in the peripheral blood of SARS-CoV-2 infected patients.
2020,
Pubmed
Mariette,
Effectiveness of Tocilizumab in Patients Hospitalized With COVID-19: A Follow-up of the CORIMUNO-TOCI-1 Randomized Clinical Trial.
2021,
Pubmed
McRae,
Programmable Bio-nanochip Platform: A Point-of-Care Biosensor System with the Capacity To Learn.
2016,
Pubmed
Mehta,
COVID-19: consider cytokine storm syndromes and immunosuppression.
2020,
Pubmed
Mehta,
Silencing the cytokine storm: the use of intravenous anakinra in haemophagocytic lymphohistiocytosis or macrophage activation syndrome.
2020,
Pubmed
Min,
Integrated Biosensor for Rapid and Point-of-Care Sepsis Diagnosis.
2018,
Pubmed
Moore,
Cytokine release syndrome in severe COVID-19.
2020,
Pubmed
Neelapu,
Chimeric antigen receptor T-cell therapy - assessment and management of toxicities.
2018,
Pubmed
Nishimoto,
Mechanisms and pathologic significances in increase in serum interleukin-6 (IL-6) and soluble IL-6 receptor after administration of an anti-IL-6 receptor antibody, tocilizumab, in patients with rheumatoid arthritis and Castleman disease.
2008,
Pubmed
Park,
Biotunable Nanoplasmonic Filter on Few-Layer MoS2 for Rapid and Highly Sensitive Cytokine Optoelectronic Immunosensing.
2017,
Pubmed
Park,
An Integrated Plasmo-Photoelectronic Nanostructure Biosensor Detects an Infection Biomarker Accompanying Cell Death in Neutrophils.
2020,
Pubmed
Pino,
Regenerative Medicine and Immunomodulatory Therapy: Insights From the Kidney, Heart, Brain, and Lung.
2018,
Pubmed
Piraino,
A Digital-Analog Microfluidic Platform for Patient-Centric Multiplexed Biomarker Diagnostics of Ultralow Volume Samples.
2016,
Pubmed
Rissin,
Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations.
2010,
Pubmed
Semler,
Identifying Clinical Research Priorities in Adult Pulmonary and Critical Care. NHLBI Working Group Report.
2020,
Pubmed
Sinha,
Is a "Cytokine Storm" Relevant to COVID-19?
2020,
Pubmed
Song,
AC Electroosmosis-Enhanced Nanoplasmofluidic Detection of Ultralow-Concentration Cytokine.
2017,
Pubmed
Sun,
Power-free polydimethylsiloxane femtoliter-sized arrays for bead-based digital immunoassays.
2019,
Pubmed
Tan,
Glass capillary based microfluidic ELISA for rapid diagnostics.
2017,
Pubmed
Wilson,
The Simoa HD-1 Analyzer: A Novel Fully Automated Digital Immunoassay Analyzer with Single-Molecule Sensitivity and Multiplexing.
2016,
Pubmed
Yelleswarapu,
Mobile platform for rapid sub-picogram-per-milliliter, multiplexed, digital droplet detection of proteins.
2019,
Pubmed
Yessayan,
Treatment of Cytokine Storm in COVID-19 Patients With Immunomodulatory Therapy.
2020,
Pubmed
Zhang,
Correction to Digital Bioassays: Theory, Applications, and Perspectives.
2017,
Pubmed
de Brito,
The balance between the serum levels of IL-6 and IL-10 cytokines discriminates mild and severe acute pneumonia.
2016,
Pubmed
