Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Dev Growth Differ
1995 Apr 01;372:191-200. doi: 10.1046/j.1440-169X.1995.t01-1-00008.x.
Show Gene links
Show Anatomy links
Ca2+ release from intracellular stores by thapsigargin in sea urchin eggs: Relationship to larval development and relevance in egg activation.
Payan P
,
Bourgeade V
,
De Renzis G
,
Girard JP
.
???displayArticle.abstract???
Thapsigargin (Tg), an inhibitor of microsomal Ca2+ ATPase, is used as a tool to study the changes in Ca2+ sequestration in sea urchin eggs and their relationship to embryonic development. Micromolar amounts of Tg inhibit ATP-dependent Ca2+ sequestration in a dose-dependent and non-reversible manner, depending on the bulk of biological material used. IC5O values are 1 nmol/L and 1-10μmol/L, respectively, in the cortical Ca2+ stores (isolated cortices preparation) and in digitonin-permeabilized eggs, a preparation giving access to the deeper reticulum compartment. Micromolar Tg does not induce Ca2+ release from 45 Ca pre-loaded cortices but leads to a loss of 25% of the total Ca2+ content from the cortical area. Using microspectrofluorimetry of fura-2-loaded eggs, we found that 10 μmol/L Tg induced a moderate rise in cytosolic Ca2+ activity as compared with the fertilization-induced Ca2+ transient whether eggs were fertilized or not. Early events related to fertilization as, for example, elevation of the fertilization envelope, proton excretion and sustained increase of amino acid uptake, are triggered by 10μmol/L Tg but with a delayed onset relative to sperm-induced effects. The present findings indicate that although it triggers most fertilization-related events, Tg cannot be considered as a true mitotic agent in sea urchin eggs. When added after fertilization, Tg affects cleavage and the further embryonic development giving rise to abnormalities comparable to the animalized larvae obtained with other compounds responsible for the inhibition of reticular Ca2+ sequestration.
Allemand,
Activation of amino acid uptake at fertilization in the sea urchin egg. Requirement for proton compartmentalization during cytosolic alkalosis.
1987, Pubmed,
Echinobase
Allemand,
Activation of amino acid uptake at fertilization in the sea urchin egg. Requirement for proton compartmentalization during cytosolic alkalosis.
1987,
Pubmed
,
Echinobase
Berridge,
Inositol trisphosphate and calcium signalling.
1993,
Pubmed
Biyiti,
Effect of antibacterial plant flavanones on the intracellular calcium compartment involved in the first cleavage of sea urchin eggs.
1990,
Pubmed
,
Echinobase
Chao,
Activation of MAP kinases by calcium-dependent and calcium-independent pathways. Stimulation by thapsigargin and epidermal growth factor.
1992,
Pubmed
Dubé,
Thapsigargin induces meiotic maturation in surf clam oocytes.
1992,
Pubmed
Ghosh,
Persistent intracellular calcium pool depletion by thapsigargin and its influence on cell growth.
1991,
Pubmed
Gillot,
Calcium in sea urchin egg during fertilization.
1990,
Pubmed
,
Echinobase
Girard,
Calcium pools in sea urchin eggs: roles of endoplasmic reticulum and mitochondria in relation to fertilization.
1991,
Pubmed
,
Echinobase
Irvine,
Inositol phosphates and Ca2+ entry: toward a proliferation or a simplification?
1992,
Pubmed
Ishikawa,
Thapsigargin increases cellular free calcium and intracellular sodium concentrations in cultured rat glomerular mesangial cells.
1993,
Pubmed
Islam,
Mobilization of Ca2+ by thapsigargin and 2,5-di-(t-butyl)-1,4-benzohydroquinone in permeabilized insulin-secreting RINm5F cells: evidence for separate uptake and release compartments in inositol 1,4,5-trisphosphate-sensitive Ca2+ pool.
1993,
Pubmed
Jaffe,
Structural changes of the endoplasmic reticulum of sea urchin eggs during fertilization.
1993,
Pubmed
,
Echinobase
Kimura,
Thapsigargin-evoked changes in human platelet Ca2+, Na+, pH and membrane potential.
1993,
Pubmed
Kline,
Thapsigargin activates a calcium influx pathway in the unfertilized mouse egg and suppresses repetitive calcium transients in the fertilized egg.
1992,
Pubmed
Lupu-Meiri,
Calcium entry in Xenopus oocytes: effects of inositol trisphosphate, thapsigargin and DMSO.
1993,
Pubmed
Metz,
Thapsigargin defines the roles of cellular calcium in secretagogue-stimulated enzyme secretion from pancreatic acini.
1992,
Pubmed
Nicaise,
The calcium loading of secretory granules. A possible key event in stimulus-secretion coupling.
1992,
Pubmed
Payan,
Uptake and release of calcium by isolated egg cortices of the sea urchin Paracentrotus lividus.
1986,
Pubmed
,
Echinobase
Sagara,
Characterization of the inhibition of intracellular Ca2+ transport ATPases by thapsigargin.
1992,
Pubmed
Steinhardt,
Activation of sea-urchin eggs by a calcium ionophore.
1974,
Pubmed
,
Echinobase
Steinhardt,
Intracellular calcium release at fertilization in the sea urchin egg.
1977,
Pubmed
,
Echinobase
Steinhardt,
Intracellular free calcium rise triggers nuclear envelope breakdown in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Takemura,
Activation of calcium entry by the tumor promoter thapsigargin in parotid acinar cells. Evidence that an intracellular calcium pool and not an inositol phosphate regulates calcium fluxes at the plasma membrane.
1989,
Pubmed
Tao,
Actions of thapsigargin on the Ca(2+)-handling systems of the human platelet. Incomplete inhibition of the dense tubular Ca2+ uptake, partial inhibition of the Ca2+ extrusion pump, increase in plasma membrane Ca2+ permeability, and consequent elevation of resting cytoplasmic Ca2+.
1992,
Pubmed
Thastrup,
Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase.
1990,
Pubmed
Törnquist,
Modulatory effect of protein kinase C on thapsigargin-induced calcium entry in thyroid FRTL-5 cells.
1993,
Pubmed
Vigne,
Thapsigargin, a new inotropic agent, antagonizes action of endothelin-1 in rat atrial cells.
1992,
Pubmed
Walter,
Mediating effect of calcium in HgCl2 cytotoxicity in sea urchin egg: role of mitochondria in Ca2+-mediated death.
1989,
Pubmed
,
Echinobase
Whitaker,
Calcium and cell cycle control.
1990,
Pubmed
,
Echinobase
Wilt,
Determination and morphogenesis in the sea urchin embryo.
1987,
Pubmed
,
Echinobase
Wrzosek,
Effect of thapsigargin on cardiac muscle cells.
1992,
Pubmed
