Dev Growth Differ 1980 Jan 01;225:749-762. doi: 10.1111/j.1440-169X.1980.00749.x.

GENERAL PATTERN, 35 SO4 -INCORPORATION AND INTRACELLULAR LOCALIZATION OF GLYCANS IN DEVELOPING SEA URCHIN (ANTHOCIDARIS) EMBRYOS.

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Glycosaminoglycan (GAG) prepared from sea urchin embryos (Anthocidaris crassispina) at various stages with or without pulse 35 SO4 -labelling was separated into various fractions by chromatography on DEAE-cellulose with a linear NaCl concentration gradient: fraction "P" (nonacidic) and fractions "A" through "F" (of increasing acidities). The 35 SO4 -radioactivity was negligible in "P" and "A", largest in "B" and "C", and decreased in the other fractions three alphabetical order. During development (hatched blastulae to gastrulae) the glycans in fractions "P" and "A" decreased in amount, whereas those in "E" and "F" increased. "E" contained heparin-like (AMPS-1) and dermatanpolysulfate-like (AMPS-2) GAG in addition to a sulfated fucogalactan-like (E1 ) glycan. Another sulfated fucogalactan-like (F1 ) glycan was found in "F". A sulfated polysialic acid-like (S1 ) glycan was found in "C". An EDTA-extract of gastrulae gave AMPS-2, E1 and F1 . The mitochondria-rich fraction gave AMPS-1, whereas the yolk granule-rich fraction gave S1 . Most of the other still unidentified components in "B", "C", and "D" appeared to be derived from glycoproteins and were mainly located in the crude yolk-mitochondrial and cytosol fractions.

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