Dev Growth Differ 1988 Oct 01;305:553-562. doi: 10.1111/j.1440-169X.1988.00553.x.

Significance of an Increase of Intracellular Adenosine Concentration for Dormancy in Starfish Blastulae: (starfish embryos/blastula/adenosine/dormancy).

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Adenosine at concentrations greater than 6 μg/ml halted embryonic development of the starfish Asterina pectinifera specifically at the 256-cell stage which corresponds to the onset of blastulation. When a fertilized egg was cultured continuously in sea water containing adenosine from fertilization, a gradual increase in intracellular concentrations of free adenosine was observed before a cessation of development took place. On the other hand, intracellular concentrations of ATP, ADP and AMP in the embryo cultured in sea water containing adenosine were nearly the same as those of an embryo cultured in sea water without adenosine. By returning the development-arrested embryo to normal sea water the embyro developed normally to the bipinnaria stage accompanied by a gradual decrease in the intracellular cencentration of adenosine. Treatment of fertilized eggs with 9-β-d-arabinofuranosyl-9H-purine-6-amine (25 μg/ml) or 2'-deoxyadenosine (10 μg/ml) halted development specifically before the onset of blastulation in an irreversible manner. Embryos treated with 3'-deoxyadenosine (50 μg/ml) shortly after fertilization developed to healthy blastulae but hatching never occurred. These results exclude the possibilities that the action of adenosine is mediated by the inhibition of DNA synthesis or RNA polyadenylylation.

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