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Int J Environ Res Public Health
2022 Nov 30;1923:. doi: 10.3390/ijerph192315994.
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Short-End Injection Capillary Electrophoresis and Multivariate Analysis for Simultaneous Determination of Heavy Metals in Passiflora incarnata Tea.
Klassen A
,
Fernandes RF
,
de Oliveira DC
,
do Nascimento MP
,
Borges MMC
,
de Oliveira MAL
,
Calixto LA
,
Borges KB
.
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An ultra-fast method for the simultaneous determination of heavy metals in Passiflora incarnata tea by capillary electrophoresis (CE) using a short-end injection combined with multivariate analysis was proposed. Separation was conducted by hydrodynamic injection (5 s at 0.5 psi) using the short-end injection procedure in a fused uncoated silica capillary (50 cm total length, 10.2 cm effective length, 50 µm i.d.) with separation time less than 2 min. An indirect UV detection at 214 nm was employed by using imidazole as a chromophore. The buffer used was 6 mmol/L hydroxybutyric acid (HIBA). The optimum conditions by full factorial with a central point were achieved by 18-crown-6 concentration (23.3 mmol L−1), voltage (+11.4 kV), methanol concentration (3.8%), and temperature (20 °C). The method showed good linearity (R2 > 0.998) for both Cd and Pb, inter-day precision of less than 14.49%, and an adequate limit of quantification only for Cd (LOQ < 0.5 µg mL−1 for Cd) based on the US Pharmacopeial Convention limit requirements for elemental impurities. After method validation, the method was applied to Passiflora incarnata tea samples from a local market. Furthermore, the developed method showed great potential for the determination of metals in other samples with proper sample preparation procedures.
#2016/25256-6 São Paulo Research Foundation (FAPESP), N/A FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais), N/A CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES), REDE-113/10; CEX-RED-0010-14 Rede Mineira de Química (RQ-MG)
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