ECB-ART-51918
Antimicrob Agents Chemother
2022 Apr 19;664:e0087521. doi: 10.1128/aac.00875-21.
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Role of ISKpn23 in blaBKC-1 Expression and Mobilization.
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This study aimed to verify the role of ISKpn23 in the expression and mobilization of blaBKC-1 and aph(3')-VIi. Five constructs related to the natural blaBKC-1 genetic background in plasmid p60136 were made and submitted for antimicrobial susceptibility testing and quantitative reverse transcription-PCR. Transposition of ISKpn23-blaBKC-1 was investigated using transposition assays involving a 9.7-kb nonconjugative plasmid carrying blaBKC-1 (p60136) and a transfer-proficient plasmid (pOX38-Gen). The presence of ISKpn23 had a crucial role in blaBKC-1 expression, resulting in increased β-lactam MICs. While we detected mobilization of p60136 by the pOX38-Gen plasmid, transposition of ISKpn23-blaBKC-1 was not observed.
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References [+] :
Derbyshire,
Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats.
1987, Pubmed