Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Braz J Microbiol
2022 Jun 01;532:785-789. doi: 10.1007/s42770-022-00699-0.
Show Gene links
Show Anatomy links
Decreased susceptibility to imipenem and ceftazidime in early virulent Raoultella spp. strains retrieved from human intestinal infections.
Valiatti TB
,
Santos FF
,
Nunes PHS
,
Streling AP
,
Veiga R
,
Cayô R
,
Gales AC
,
Gomes TAT
.
???displayArticle.abstract???
The genus Raoultella spp. is comprised of four species, namely, R. electrica, R. ornithinolytica, R. planticola, and R. terrigena, which are rarely reported to cause infections in humans. This study aimed to characterize six strains of Raoultella spp. isolated from stool samples from patients with diarrhea. The strains included in the study were previously identified by biochemical methods as K. pneumoniae, during a surveillance study conducted in 1987. In the present study, the strains were re-identified by MALDI TOF and 16S rRNA sequencing and subsequently subjected to virulence gene screening by PCR, hemolytic activity, biofilm formation, hypermucoviscosity phenotype, capacity to interact with Caco-2 cells, and antimicrobial susceptibility test. Our results revealed that, among the six strains, three were identified as R. ornithinolytica and three as R. planticola. The genes related to iron uptake systems (aero1, aero2, iutA, entB, and ybtS) and adhesin (mrkD) were found in all strains. Furthermore, all strains demonstrated the ability to interact in vitro with Caco-2 cells and form biofilms. In general, the strains studied were sensitive to the antimicrobials tested; however, it was possible to observe high MICs for imipenem compared to ertapenem and meropenem and high minimal inhibitory concentrations (MICs) for ceftazidime, except for one strain. Our results show the occurrence of virulent strains of Raoultella spp. with high MICs for imipenem and ceftazidime causing diarrhea. We hope that our findings can contribute to the understanding of the evolution of this species since, as far as we know, these are the oldest isolates reported so far.
Agüero,
A plasmid-encoded outer membrane protein, TraT, enhances resistance of Escherichia coli to phagocytosis.
1984, Pubmed
Agüero,
A plasmid-encoded outer membrane protein, TraT, enhances resistance of Escherichia coli to phagocytosis.
1984,
Pubmed
Alampoondi Venkataramanan,
A 5-Year Retrospective Analysis of Raoultella planticola Bacteriuria.
2021,
Pubmed
Appel,
Microbiological and Clinical Aspects of Raoultella spp.
2021,
Pubmed
Beutin,
Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli.
1989,
Pubmed
Boattini,
Infections on the rise: Raoultella spp., clinical and microbiological findings from a retrospective study, 2010-2014.
2016,
Pubmed
Cortés-Ortíz,
Multidrug-resistant Raoultella ornithinolytica misidentified as Klebsiella oxytoca carrying blaOXA β-lactamases: antimicrobial profile and genomic characterization.
2021,
Pubmed
Drancourt,
Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov.
2001,
Pubmed
El Fertas-Aissani,
Virulence profiles and antibiotic susceptibility patterns of Klebsiella pneumoniae strains isolated from different clinical specimens.
2013,
Pubmed
Fang,
A novel virulence gene in Klebsiella pneumoniae strains causing primary liver abscess and septic metastatic complications.
2004,
Pubmed
Hajjar,
Raoultella ornithinolytica: Emergence and Resistance.
2020,
Pubmed
Høiby,
The clinical impact of bacterial biofilms.
2011,
Pubmed
Johnson,
Type 3 fimbriae and biofilm formation are regulated by the transcriptional regulators MrkHI in Klebsiella pneumoniae.
2011,
Pubmed
Kanukollu,
Contribution of the traT gene to serum resistance among clinical isolates of enterobacteriaceae.
1985,
Pubmed
Lima,
Phenotypic characterization and virulence-related properties of Escherichia albertii strains isolated from children with diarrhea in Brazil.
2019,
Pubmed
Moll,
Plasmid-determined resistance to serum bactericidal activity: a major outer membrane protein, the traT gene product, is responsible for plasmid-specified serum resistance in Escherichia coli.
1980,
Pubmed
Narisawa,
Coexistence of antibiotic-producing and antibiotic-sensitive bacteria in biofilms is mediated by resistant bacteria.
2008,
Pubmed
Park,
Evaluation of three phenotypic identification systems for clinical isolates of Raoultella ornithinolytica.
2011,
Pubmed
Podschun,
Expression of putative virulence factors by clinical isolates of Klebsiella planticola.
2000,
Pubmed
Ponce-Alonso,
Comparison of different methods for identification of species of the genus Raoultella: report of 11 cases of Raoultella causing bacteraemia and literature review.
2016,
Pubmed
Stepanovic,
A modified microtiter-plate test for quantification of staphylococcal biofilm formation.
2000,
Pubmed
Sêkowska,
Raoultella spp. - reliable identification, susceptibility to antimicrobials and antibiotic resistance mechanisms.
2020,
Pubmed
Sękowska,
Raoultella spp.-clinical significance, infections and susceptibility to antibiotics.
2017,
Pubmed
Tufa,
CTX-M-9 group ESBL-producing Raoultella planticola nosocomial infection: first report from sub-Saharan Africa.
2020,
Pubmed
Valiatti,
Characterization of virulent Klebsiella variicola recovered from inpatients with intestinal and extraintestinal infections between 1987 and 1999.
2021,
Pubmed
Valiatti,
Genetic and Virulence Characteristics of a Hybrid Atypical Enteropathogenic and Uropathogenic Escherichia coli (aEPEC/UPEC) Strain.
2020,
Pubmed
Versalovic,
Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes.
1991,
Pubmed
Weisburg,
16S ribosomal DNA amplification for phylogenetic study.
1991,
Pubmed
Yu,
Isolation and identification of a new tetrodotoxin-producing bacterial species, Raoultella terrigena, from Hong Kong marine puffer fish Takifugu niphobles.
2011,
Pubmed
Yu,
Comparison of prevalence of virulence factors for Klebsiella pneumoniae liver abscesses between isolates with capsular K1/K2 and non-K1/K2 serotypes.
2008,
Pubmed
de Jong,
Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
2013,
Pubmed