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Hillberg AK
,
Smith MK
,
Lausen BS
,
Suwansa-Ard S
,
Johnston R
,
Mitu SA
,
MacDonald LE
,
Zhao M
,
Motti CA
,
Wang T
,
Elizur A
,
Nakashima K
,
Satoh N
,
Cummins SF
.
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BACKGROUND: The crown-of-thorns starfish (COTS; Acanthaster species) is a slow-moving corallivore protected by an extensive array of long, sharp toxic spines. Envenomation can result in nausea, numbness, vomiting, joint aches and sometimes paralysis. Small molecule saponins and the plancitoxin proteins have been implicated in COTS toxicity.
METHODS: Brine shrimp lethality assays were used to confirm the secretion of spine toxin biomolecules. Histological analysis, followed by spine-derived proteomics helped to explain the source and identity of proteins, while quantitative RNA-sequencing and phylogeny confirmed target gene expression and relative conservation, respectively.
RESULTS: We demonstrate the lethality of COTS spine secreted biomolecules on brine shrimp, including significant toxicity using aboral spine semi-purifications of >10 kDa (p > 0.05, 9.82 µg/ml), supporting the presence of secreted proteins as toxins. Ultrastructure observations of the COTS aboral spine showed the presence of pores that could facilitate the distribution of secreted proteins. Subsequent purification and mass spectrometry analysis of spine-derived proteins identified numerous secretory proteins, including plancitoxins, as well as those with relatively high gene expression in spines, including phospholipase A2, protease inhibitor 16-like protein, ependymin-related proteins and those uncharacterized. Some secretory proteins (e.g., vitellogenin and deleted in malignant brain tumor protein 1) were not highly expressed in spine tissue, yet the spine may serve as a storage or release site. This study contributes to our understanding of the COTS through functional, ultrastructural and proteomic analysis of aboral spines.
Abraham,
Tracing the Evolutionary History of the CAP Superfamily of Proteins Using Amino Acid Sequence Homology and Conservation of Splice Sites.
2017, Pubmed
Abraham,
Tracing the Evolutionary History of the CAP Superfamily of Proteins Using Amino Acid Sequence Homology and Conservation of Splice Sites.
2017,
Pubmed
Almagro Armenteros,
SignalP 5.0 improves signal peptide predictions using deep neural networks.
2019,
Pubmed
Alqaisi,
A comparative study of vitellogenesis in Echinodermata: Lessons from the sea star.
2016,
Pubmed
,
Echinobase
Americus,
The cnidarian parasite Ceratonova shasta utilizes inherited and recruited venom-like compounds during infection.
2021,
Pubmed
Boyanovsky,
Biology of secretory phospholipase A2.
2009,
Pubmed
Byrne,
The evolution of egg yolk proteins.
1989,
Pubmed
De Marino,
Starfish saponins. 55. Isolation, structure elucidation, and biological activity of the steroid oligoglycosides from an Antarctic starfish of the family Asteriidae.
1998,
Pubmed
,
Echinobase
Demeyer,
Inter- and intra-organ spatial distributions of sea star saponins by MALDI imaging.
2015,
Pubmed
,
Echinobase
Gaikwad,
The functions of CAP superfamily proteins in mammalian fertility and disease.
2020,
Pubmed
Hall,
The crown-of-thorns starfish genome as a guide for biocontrol of this coral reef pest.
2017,
Pubmed
,
Echinobase
Hazell,
PI16 is a shear stress and inflammation-regulated inhibitor of MMP2.
2016,
Pubmed
Hope,
Peptidase inhibitor 16 identifies a human regulatory T-cell subset with reduced FOXP3 expression over the first year of recent onset type 1 diabetes.
2019,
Pubmed
Ihama,
Anaphylactic shock caused by sting of crown-of-thorns starfish (Acanthaster planci).
2014,
Pubmed
,
Echinobase
Karasudani,
Purification of anticoagulant factor from the spine venom of the crown-of-thorns starfish, Acanthaster planci.
1996,
Pubmed
,
Echinobase
Kelley,
Renaturation of cobra venom phospholipase A2 expressed from a synthetic gene in Escherichia coli.
1992,
Pubmed
Konshina,
Anionic lipids: determinants of binding cytotoxins from snake venom on the surface of cell membranes.
2010,
Pubmed
Krogh,
Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
2001,
Pubmed
Lambeau,
Receptors for a growing family of secreted phospholipases A2.
1999,
Pubmed
Lambeau,
Identification and purification of a very high affinity binding protein for toxic phospholipases A2 in skeletal muscle.
1990,
Pubmed
Lee,
Antioxidative and anticancer activities of various ethanolic extract fractions from crown-of-thorns starfish (Acanthaster planci).
2014,
Pubmed
,
Echinobase
Lee,
Spine venom of crown-of-thorns starfish (Acanthaster planci) induces antiproliferation and apoptosis of human melanoma cells (A375.S2).
2014,
Pubmed
,
Echinobase
Lee,
Hemolytic activity of venom from crown-of-thorns starfish Acanthaster planci spines.
2013,
Pubmed
,
Echinobase
Leonardi,
Conus consors snail venom proteomics proposes functions, pathways, and novel families involved in its venomic system.
2012,
Pubmed
Letunic,
Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation.
2021,
Pubmed
Lu,
Sequence analysis and antigenic cross-reactivity of a venom allergen, antigen 5, from hornets, wasps, and yellow jackets.
1993,
Pubmed
McDougall,
The evolution of ependymin-related proteins.
2018,
Pubmed
,
Echinobase
McIntosh,
Conodipine-M, a novel phospholipase A2 isolated from the venom of the marine snail Conus magus.
1995,
Pubmed
Metsalu,
ClustVis: a web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap.
2015,
Pubmed
Meyer,
Brine shrimp: a convenient general bioassay for active plant constituents.
1982,
Pubmed
Ngoan,
Asterosaponins and glycosylated polyhydroxysteroids from the starfish Culcita novaeguineae and their cytotoxic activities.
2015,
Pubmed
,
Echinobase
Ni,
Comparative Proteomic Study of the Antiproliferative Activity of Frog Host-Defence Peptide Caerin 1.9 and Its Additive Effect with Caerin 1.1 on TC-1 Cells Transformed with HPV16 E6 and E7.
2018,
Pubmed
Nicholson,
PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20.
2012,
Pubmed
Ota,
Molecular cloning of two toxic phospholipases A2 from the crown-of-thorns starfish Acanthaster planci venom.
2006,
Pubmed
,
Echinobase
Regn,
Peptidase inhibitor 16 is a membrane-tethered regulator of chemerin processing in the myocardium.
2016,
Pubmed
Roberts,
Identification of putative olfactory G-protein coupled receptors in Crown-of-Thorns starfish, Acanthaster planci.
2017,
Pubmed
,
Echinobase
Sato,
Case of skin injuries due to stings by crown-of-thorns starfish (Acanthaster planci).
2008,
Pubmed
,
Echinobase
Schaloske,
The phospholipase A2 superfamily and its group numbering system.
2006,
Pubmed
Shi,
Vitellogenin is a novel player in defense reactions.
2006,
Pubmed
Shiomi,
Purification and properties of phospholipases A2 from the crown-of-thorns starfish (Acanthaster planci) venom.
1998,
Pubmed
,
Echinobase
Shiomi,
Plancitoxins, lethal factors from the crown-of-thorns starfish Acanthaster planci, are deoxyribonucleases II.
2004,
Pubmed
,
Echinobase
Shiomi,
Purification and characterization of a lethal factor in venom from the crown-of-thorns starfish (Acanthaster planci).
1988,
Pubmed
,
Echinobase
Shiomi,
Liver damage by the crown-of-thorns starfish (Acanthaster planci) lethal factor.
1990,
Pubmed
,
Echinobase
Shiroma,
Haemodynamic and haematologic effects of Acanthaster planci venom in dogs.
1994,
Pubmed
,
Echinobase
Singh,
Vitellogenin from the silkworm, Bombyx mori: an effective anti-bacterial agent.
2013,
Pubmed
Singhmar,
The fibroblast-derived protein PI16 controls neuropathic pain.
2020,
Pubmed
Six,
The expanding superfamily of phospholipase A(2) enzymes: classification and characterization.
2000,
Pubmed
Smith,
Differences in Small Molecule Neurotransmitter Profiles From the Crown-of-Thorns Seastar Radial Nerve Revealed Between Sexes and Following Food-Deprivation.
2018,
Pubmed
,
Echinobase
Smith,
The neuropeptidome of the Crown-of-Thorns Starfish, Acanthaster planci.
2017,
Pubmed
,
Echinobase
Sun,
Immune-Relevant and Antioxidant Activities of Vitellogenin and Yolk Proteins in Fish.
2015,
Pubmed
Tadokoro,
Cysteine-Rich Secretory Proteins (CRISPs) From Venomous Snakes: An Overview of the Functional Diversity in A Large and Underappreciated Superfamily.
2020,
Pubmed
Thao,
Anti-inflammatory asterosaponins from the starfish Astropecten monacanthus.
2013,
Pubmed
,
Echinobase
Wei,
Crystal structures of human lysosomal EPDR1 reveal homology with the superfamily of bacterial lipoprotein transporters.
2019,
Pubmed
Yara,
Cardiovascular effects of Acanthaster planci venom in the rat: possible involvement of PAF in its hypotensive effect.
1992,
Pubmed
,
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