Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
PLoS One
2023 Jan 01;189:e0289895. doi: 10.1371/journal.pone.0289895.
Show Gene links
Show Anatomy links
Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm.
Duan X
,
Jiang L
,
Guo M
,
Li C
.
???displayArticle.abstract???
Vibrio splendidus is a common pathogen in the ocean that infects Apostichopus japonicus, Atlantic salmon and Crassostrea gigas, leading to a variety of diseases. In this study, a virulent phage vB_VspM_VS1, which infects V. splendidus, was isolated from aquaculture ponds in Dalian, China, and it belongs to the family Straboviridae in the order Caudoviricetes. vB_VspM_VS1 had an adsorption rate of 96% in 15 min, a latent period of 65 min, and a burst size of 140 ± 6 PFU/cell. The complete genome of phage vB_VspM_VS1 consists of a linear double-stranded DNA that is 248,270 bp in length with an average G + C content of 42.5% and 389 putative protein-coding genes; 116 genes have known functions. There are 4 tail fiber genes in the positive and negative strands of the phage vB_VspM_VS1 genome. The protein domain of the phage vB_VspM_VS1 tail fibers was obtained from the Protein Data Bank and the SMART (http://smart.embl.de) database. Bacterial challenge tests revealed that the growth of V. splendidus HS0 was apparently inhibited (OD600 < 0.01) in 12 h at an MOI of 10. In against biofilms, we also showed that the OD570 value of the vB_VspM_VS1-treated group (MOI = 1) decreased significantly to 0.04 ± 0.01 compared with that of the control group (0.48 ± 0.08) at 24 h. This study characterizes the genome of the phage vB_VspM_VS1 that infects the pathogenic bacterium V. splendidus of A. japonicus.
???displayArticle.pubmedLink???
37656737
???displayArticle.link???PLoS One
Azam,
Bacteriophage-host arm race: an update on the mechanism of phage resistance in bacteria and revenge of the phage with the perspective for phage therapy.
2019, Pubmed
Azam,
Bacteriophage-host arm race: an update on the mechanism of phage resistance in bacteria and revenge of the phage with the perspective for phage therapy.
2019,
Pubmed
Bernheim,
The pan-immune system of bacteria: antiviral defence as a community resource.
2020,
Pubmed
Davies,
Understanding biofilm resistance to antibacterial agents.
2003,
Pubmed
Davin-Regli,
Enterobacter aerogenes and Enterobacter cloacae; versatile bacterial pathogens confronting antibiotic treatment.
2015,
Pubmed
Davin-Regli,
Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance.
2019,
Pubmed
Divya Ganeshan,
Phage Therapy with a Focus on the Human Microbiota.
2019,
Pubmed
Gatesoupe,
Pathogenicity of vibrio splendidus strains associated with turbot larvae, scophthalmus maximus.
1999,
Pubmed
Hatfull,
Bacteriophage genomics.
2008,
Pubmed
Katharios,
Isolation and characterization of a N4-like lytic bacteriophage infecting Vibrio splendidus, a pathogen of fish and bivalves.
2017,
Pubmed
Kumar,
Antibiotics bioremediation: Perspectives on its ecotoxicity and resistance.
2019,
Pubmed
Kutateladze,
Bacteriophages as potential new therapeutics to replace or supplement antibiotics.
2010,
Pubmed
Li,
Vibrio splendidus persister cells induced by host coelomic fluids show a similar phenotype to antibiotic-induced counterparts.
2021,
Pubmed
,
Echinobase
Li,
Use of phages to control Vibrio splendidus infection in the juvenile sea cucumber Apostichopus japonicus.
2016,
Pubmed
,
Echinobase
Liu,
Identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers Apostichopus japonicus (Selenka).
2010,
Pubmed
,
Echinobase
Miernikiewicz,
T4 Phage Tail Adhesin Gp12 Counteracts LPS-Induced Inflammation In Vivo.
2016,
Pubmed
Mishra,
Expression and lytic efficacy assessment of the Staphylococcus aureus phage SA4 lysin gene.
2013,
Pubmed
Park,
Characterization and comparative genomic analysis of a novel bacteriophage, SFP10, simultaneously inhibiting both Salmonella enterica and Escherichia coli O157:H7.
2012,
Pubmed
Yehl,
Engineering Phage Host-Range and Suppressing Bacterial Resistance through Phage Tail Fiber Mutagenesis.
2019,
Pubmed
Zhang,
Inhibition of marine Vibrio sp. by pyoverdine from Pseudomonas aeruginosa PA1.
2016,
Pubmed
,
Echinobase
Zhang,
Bacteriophage P1 does not show spatial preference when infecting Escherichia coli.
2020,
Pubmed