Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Front Genet
2023 Jan 01;14:1097825. doi: 10.3389/fgene.2023.1097825.
Show Gene links
Show Anatomy links
Identification and functional analysis of Dmrt1 gene and the SoxE gene in the sexual development of sea cucumber, Apostichopus japonicus.
Liu BZ
,
Cong JJ
,
Su WY
,
Hao ZL
,
Sun ZH
,
Chang YQ
.
???displayArticle.abstract???
Members of the Doublesex and Mab-3-related transcription factor (Dmrt) gene family handle various vital functions in several biological processes, including sex determination/differentiation and gonad development. Dmrt1 and Sox9 (SoxE in invertebrates) exhibit a very conserved interaction function during testis formation in vertebrates. However, the dynamic expression pattern and functional roles of the Dmrt gene family and SoxE have not yet been identified in any echinoderm species. Herein, five members of the Dmrt gene family (Dmrt1, 2, 3a, 3b and 5) and the ancestor SoxE gene were identified from the genome of Apostichopus japonicus. Expression studies of Dmrt family genes and SoxE in different tissues of adult males and females revealed different expression patterns of each gene. Transcription of Dmrt2, Dmrt3a and Dmrt3b was higher expressed in the tube feet and coelomocytes instead of in gonadal tissues. The expression of Dmrt1 was found to be sustained throughout spermatogenesis. Knocking-down of Dmrt1 by means of RNA interference (RNAi) led to the downregulation of SoxE and upregulation of the ovarian regulator foxl2 in the testes. This indicates that Dmrt1 may be a positive regulator of SoxE and may play a role in the development of the testes in the sea cucumber. The expression level of SoxE was higher in the ovaries than in the testes, and knocking down of SoxE by RNAi reduced SoxE and Dmrt1 expression but conversely increased the expression of foxl2 in the testes. In summary, this study indicates that Dmrt1 and SoxE are indispensable for testicular differentiation, and SoxE might play a functional role during ovary differentiation in the sea cucumber.
Amterat Abu Abayed,
Screening for Dmrt genes from embryo to mature Macrobrachium rosenbergii prawns.
2019, Pubmed
Amterat Abu Abayed,
Screening for Dmrt genes from embryo to mature Macrobrachium rosenbergii prawns.
2019,
Pubmed
Barrionuevo,
Homozygous inactivation of Sox9 causes complete XY sex reversal in mice.
2006,
Pubmed
Bellefroid,
Expanding roles for the evolutionarily conserved Dmrt sex transcriptional regulators during embryogenesis.
2013,
Pubmed
Bewick,
Evolution of the closely related, sex-related genes DM-W and DMRT1 in African clawed frogs (Xenopus).
2011,
Pubmed
Burtis,
Drosophila doublesex gene controls somatic sexual differentiation by producing alternatively spliced mRNAs encoding related sex-specific polypeptides.
1989,
Pubmed
Chakraborty,
Dmy initiates masculinity by altering Gsdf/Sox9a2/Rspo1 expression in medaka (Oryzias latipes).
2016,
Pubmed
Cui,
Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis).
2017,
Pubmed
Dong,
Comparative Genomics Studies on the dmrt Gene Family in Fish.
2020,
Pubmed
Du,
Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis.
2019,
Pubmed
Elzaiat,
High-throughput sequencing analyses of XX genital ridges lacking FOXL2 reveal DMRT1 up-regulation before SOX9 expression during the sex-reversal process in goats.
2014,
Pubmed
Ge,
Dmrt1 induces the male pathway in a turtle species with temperature-dependent sex determination.
2017,
Pubmed
Gonzalez,
Maternal Piwi regulates primordial germ cell development to ensure the fertility of female progeny in Drosophila.
2021,
Pubmed
Heenan,
Evolution of the Sox gene family within the chordate phylum.
2016,
Pubmed
Hui,
Sox9 is indispensable for testis differentiation in the red-eared slider turtle, a reptile with temperature-dependent sex determination.
2021,
Pubmed
Jakob,
Sex determination and the control of Sox9 expression in mammals.
2011,
Pubmed
Jiang,
Comparative analysis of immunocompetence between females and males in the sea cucumber Apostichopus japonicus.
2017,
Pubmed
,
Echinobase
Jiang,
Gender specific differences of immune competence in the sea cucumber Apostichopus japonicus before and after spawning.
2019,
Pubmed
,
Echinobase
Johnsen,
Sex dimorphic expression of five dmrt genes identified in the Atlantic cod genome. The fish-specific dmrt2b diverged from dmrt2a before the fish whole-genome duplication.
2012,
Pubmed
Kent,
A male-specific role for SOX9 in vertebrate sex determination.
1996,
Pubmed
Kim,
Sexually dimorphic expression of multiple doublesex-related genes in the embryonic mouse gonad.
2003,
Pubmed
Kim,
Cell type-autonomous and non-autonomous requirements for Dmrt1 in postnatal testis differentiation.
2007,
Pubmed
Kopp,
Dmrt genes in the development and evolution of sexual dimorphism.
2012,
Pubmed
Krentz,
The DM domain protein DMRT1 is a dose-sensitive regulator of fetal germ cell proliferation and pluripotency.
2009,
Pubmed
Li,
Nuclear localization, DNA binding and restricted expression in neural and germ cells of zebrafish Dmrt3.
2008,
Pubmed
Li,
Transcriptome Sequencing and Comparative Analysis of Ovary and Testis Identifies Potential Key Sex-Related Genes and Pathways in Scallop Patinopecten yessoensis.
2016,
Pubmed
Li,
Sea cucumber genome provides insights into saponin biosynthesis and aestivation regulation.
2018,
Pubmed
,
Echinobase
Lindeman,
The conserved sex regulator DMRT1 recruits SOX9 in sexual cell fate reprogramming.
2021,
Pubmed
Luo,
Direct production of XY(DMY-) sex reversal female medaka (Oryzias latipes) by embryo microinjection of TALENs.
2015,
Pubmed
Marchand,
DMRT1 expression during gonadal differentiation and spermatogenesis in the rainbow trout, Oncorhynchus mykiss.
2000,
Pubmed
Matson,
DMRT1 prevents female reprogramming in the postnatal mammalian testis.
2011,
Pubmed
McCauley,
SoxE, Type II collagen, and evolution of the chondrogenic neural crest.
2008,
Pubmed
Ottolenghi,
Novel paralogy relations among human chromosomes support a link between the phylogeny of doublesex-related genes and the evolution of sex determination.
2002,
Pubmed
Ottolenghi,
Foxl2 is required for commitment to ovary differentiation.
2005,
Pubmed
Paul-Prasanth,
Knock-down of DMY initiates female pathway in the genetic male medaka, Oryzias latipes.
2006,
Pubmed
Santerre,
Cg-SoxE and Cg-β-catenin, two new potential actors of the sex-determining pathway in a hermaphrodite lophotrochozoan, the Pacific oyster Crassostrea gigas.
2014,
Pubmed
Sheng,
Identification of Dmrt genes and their up-regulation during gonad transformation in the swamp eel (Monopterus albus).
2014,
Pubmed
Su,
Transcriptional variants of Dmrt1 and expression of four Dmrt genes in the blunt snout bream, Megalobrama amblycephala.
2015,
Pubmed
Sun,
Identification and expression analysis of two Toll-like receptor genes from sea cucumber (Apostichopus japonicus).
2013,
Pubmed
,
Echinobase
Sun,
Identification and functional characterization of piwi1 gene in sea cucumber, Apostichopus japonicas.
2021,
Pubmed
,
Echinobase
Sun,
Identification and functional analysis of foxl2 and nodal in sea cucumber, Apostichopus japonicus.
2022,
Pubmed
,
Echinobase
Tian,
Establishment of lysozyme gene RNA interference systemand its involvement in salinity tolerance in sea cucumber (Apostichopus japonicus).
2017,
Pubmed
,
Echinobase
Tian,
Genome-wide analysis of the NRAMP gene family in potato (Solanum tuberosum): Identification, expression analysis and response to five heavy metals stress.
2021,
Pubmed
Vining,
Diverse Regulation but Conserved Function: SOX9 in Vertebrate Sex Determination.
2021,
Pubmed
Wan,
Discovery of the Dmrt gene family members based on transcriptome analysis in mud crab Scylla paramamosain.
2021,
Pubmed
Wang,
Tuatara (Sphenodon) genomics: BAC library construction, sequence survey, and application to the DMRT gene family.
2006,
Pubmed
Wang,
The DMRT gene family in amphioxus.
2012,
Pubmed
Wang,
Characterization and expression analysis of microRNAs in the tube foot of sea cucumber Apostichopus japonicus.
2014,
Pubmed
,
Echinobase
Watanabe,
Conservatism and variability of gene expression profiles among homeologous transcription factors in Xenopus laevis.
2017,
Pubmed
Wei,
Dmrt1 directly regulates the transcription of the testis-biased Sox9b gene in Nile tilapia (Oreochromis niloticus).
2019,
Pubmed
Wexler,
Pan-metazoan phylogeny of the DMRT gene family: a framework for functional studies.
2014,
Pubmed
Xu,
Genome-wide identification and characterization of DEAD-box helicase family associated with early somatic embryogenesis in Dimocarpus longan Lour.
2021,
Pubmed
Yamaguchi,
Expression of the DMRT gene and its roles in early gonadal development of the Japanese pufferfish Takifugu rubripes.
2006,
Pubmed
Yan,
Expression pattern of vasa in gonads of sea cucumber Apostichopus japonicus during gametogenesis and reproductive cycle.
2013,
Pubmed
,
Echinobase
Yang,
A novel role of Krüppel-like factor 4 in Zhikong scallop Chlamys farreri during spermatogenesis.
2017,
Pubmed
Yang,
Genome-wide analysis of the Chinese sturgeon sox gene family: identification, characterisation and expression profiles of different tissues.
2020,
Pubmed
Yu,
Genome-wide identification and expression profiling of the SOX gene family in a bivalve mollusc Patinopecten yessoensis.
2017,
Pubmed
Zafar,
Genome-Wide identification of doublesex and Mab-3-Related transcription factor (DMRT) genes in nile tilapia (oreochromis niloticus).
2019,
Pubmed
Zhang,
The sea cucumber genome provides insights into morphological evolution and visceral regeneration.
2017,
Pubmed
,
Echinobase
Zhang,
Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus.
2019,
Pubmed
,
Echinobase
