Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Front Pharmacol
2023 Jan 01;14:1109084. doi: 10.3389/fphar.2023.1109084.
Show Gene links
Show Anatomy links
Pharmacokinetic study of Strongylocentrotus nudus egg polysaccharide in rats and beagles using a 3H-labeling method.
Xing H
,
Zhu X
,
Liao J
,
Kong Y
,
Lu Y
,
Zhao D
,
Li N
,
Chen X
,
Qin Z
.
???displayArticle.abstract???
Strongylocentrotus nudus egg polysaccharide (SEP) extracted from sea urchins has potential anticancer activity. However, little is known about its pharmacokinetic properties. To investigate the pharmacokinetics of SEP, it was radiolabeled with tritium. Furthermore, a sensitive, selective, and rapid liquid scintillation counter (LSC) method for quantifying 3H-SEP in biological matrix was validated. The lower quantification limit of the method was 4 Bq. The relative standard deviations (RSDs) of the intra- and inter-day precision were <3.0% and <3.9%, respectively. 3H-SEP was successfully applied to investigate the pharmacokinetics of SEP after intravenous administration of 20, 40, and 80 mg/kg (40 μCi/kg) in rats and 5, 10, and 20 mg/kg (6 μCi/kg) in beagles. The AUC(0-t) of SEP at three different doses was 487.81 ± 39.99 mg/L*h, 1,003.10 ± 95.94 mg/L*h, and 2,188.84 ± 137.73 mg/L*h in rats and 144.12 ± 3.78 mg/L*h, 322.62 ± 28.03 mg/L*h, and 754.17 ± 37.79 mg/L*h in beagles. The terminal elimination half-life (t1/2) of SEP was longer in beagles (204.29 ± 139.34 h) than in rats (35.48 ± 6.04 h). The concentration of SEP in plasma declined rapidly in both rats and beagles. All the study results provide detailed pharmacokinetic profiles of SEP in two kinds of animals, which will be helpful for further development.
FIGURE 2. Logarithmic graph of mean plasma concentration–time profiles in rats after intravenous injection of SEP (single doses of 20, 40, or 80 mg/kg or multiple doses of 40 mg/kg on the seventh day).
FIGURE 3. Logarithmic graph of mean plasma concentration–time profiles in beagles after intravenous injection of SEP (single doses of 5, 10, or 20 mg/kg or multiple doses of 10 mg/kg on the seventh day).
Behranvand,
Chemotherapy: a double-edged sword in cancer treatment.
2022, Pubmed
Behranvand,
Chemotherapy: a double-edged sword in cancer treatment.
2022,
Pubmed
Chang,
Bioactive polysaccharides from traditional Chinese medicine herbs as anticancer adjuvants.
2002,
Pubmed
Dalvie,
Recent advances in the applications of radioisotopes in drug metabolism, toxicology and pharmacokinetics.
2000,
Pubmed
de Jesus Raposo,
Marine polysaccharides from algae with potential biomedical applications.
2015,
Pubmed
Edelmann,
Radiolabelling small and biomolecules for tracking and monitoring.
2022,
Pubmed
Fu,
Molecular imaging of MDM2 messenger RNA with 99mTc-labeled antisense oligonucleotides in experimental human breast cancer xenografts.
2010,
Pubmed
Jiang,
Preclinical Pharmacokinetic Studies of the Tritium Labelled D-Enantiomeric Peptide D3 Developed for the Treatment of Alzheimer´s Disease.
2015,
Pubmed
Khalifa,
Marine Natural Products: A Source of Novel Anticancer Drugs.
2019,
Pubmed
Li,
Quercetin enhances chemotherapeutic effect of doxorubicin against human breast cancer cells while reducing toxic side effects of it.
2018,
Pubmed
Liu,
Immunomodulatory activity of polysaccharides isolated from Strongylocentrotus nudus eggs.
2008,
Pubmed
,
Echinobase
Ooi,
Immunomodulation and anti-cancer activity of polysaccharide-protein complexes.
2000,
Pubmed
Ren,
Marine Natural Products: A Potential Source of Anti-hepatocellular Carcinoma Drugs.
2021,
Pubmed
Wang,
Strongylocentrotus nudos Egg Polysaccharide induces autophagy and apoptosis in leukaemia cells by regulating mitochondrial function.
2021,
Pubmed
,
Echinobase
Wang,
A polysaccharide from Strongylocentrotus nudus eggs protects against myelosuppression and immunosuppression in cyclophosphamide-treated mice.
2011,
Pubmed
,
Echinobase
Wang,
Effective inhibition of a Strongylocentrotus nudus eggs polysaccharide against hepatocellular carcinoma is mediated via immunoregulation in vivo.
2011,
Pubmed
,
Echinobase
Xie,
Polysaccharide enhanced NK cell cytotoxicity against pancreatic cancer via TLR4/MAPKs/NF-κB pathway in vitro/vivo.
2019,
Pubmed
,
Echinobase
Yang,
Pharmacokinetics study of calf thymus DNA in rats and beagle dogs with (3)H-labeling and tracing method.
2014,
Pubmed
Yang,
Pharmacokinetics and disposition study of calf thymus DNA in rats by applying 3H-labeling method.
2012,
Pubmed
Zhang,
A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes.
2017,
Pubmed
,
Echinobase
Zhu,
The Synthesis of Nano-Doxorubicin and its Anticancer Effect.
2021,
Pubmed
Zong,
Anticancer polysaccharides from natural resources: a review of recent research.
2012,
Pubmed
Zraik,
[Management of chemotherapy side effects and their long-term sequelae].
2021,
Pubmed
