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Inhibition of Cytomegalovirus by Pentacta pygmaea Fucosylated Chondroitin Sulfate Depends on Its Molecular Weight.
Sharma P
,
Dwivedi R
,
Ray P
,
Shukla J
,
Pomin VH
,
Tandon R
.
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Many viruses attach to host cells by first interacting with cell surface proteoglycans containing heparan sulfate (HS) glycosaminoglycan chains and then by engaging with specific receptor, resulting in virus entry. In this project, HS-virus interactions were targeted by a new fucosylated chondroitin sulfate from the sea cucumber Pentacta pygmaea (PpFucCS) in order to block human cytomegalovirus (HCMV) entry into cells. Human foreskin fibroblasts were infected with HCMV in the presence of PpFucCS and its low molecular weight (LMW) fractions and the virus yield at five days post-infection was assessed. The virus attachment and entry into the cells were visualized by labeling the purified virus particles with a self-quenching fluorophore octadecyl rhodamine B (R18). The native PpFucCS exhibited potent inhibitory activity against HCMV specifically blocking virus entry into the cell and the inhibitory activities of the LMW PpFucCS derivatives were proportional to their chain lengths. PpFucCS and the derived oligosaccharides did not exhibit any significant cytotoxicity; moreover, they protected the infected cells from virus-induced lytic cell death. In conclusion, PpFucCS inhibits the entry of HCMV into cells and the high MW of this carbohydrate is a key structural element to achieve the maximal anti-viral effect. This new marine sulfated glycan can be developed into a potential prophylactic and therapeutic antiviral agent against HCMV infection.
Aquino,
Glycosaminoglycans and infection.
2016, Pubmed
Aquino,
Glycosaminoglycans and infection.
2016,
Pubmed
Badani,
Peptide entry inhibitors of enveloped viruses: the importance of interfacial hydrophobicity.
2014,
Pubmed
Baldwin,
A role for 3-O-sulfated heparan sulfate in promoting human cytomegalovirus infection in human iris cells.
2015,
Pubmed
Borst,
A peptide inhibitor of cytomegalovirus infection from human hemofiltrate.
2013,
Pubmed
Britt,
Congenital Human Cytomegalovirus Infection and the Enigma of Maternal Immunity.
2017,
Pubmed
Cagno,
Heparan Sulfate Proteoglycans and Viral Attachment: True Receptors or Adaptation Bias?
2019,
Pubmed
Chou,
Approach to drug-resistant cytomegalovirus in transplant recipients.
2015,
Pubmed
Compton,
Initiation of human cytomegalovirus infection requires initial interaction with cell surface heparan sulfate.
1993,
Pubmed
Dunn,
Functional profiling of a human cytomegalovirus genome.
2003,
Pubmed
Dwivedi,
Structural and kinetic analyses of holothurian sulfated glycans suggest potential treatment for SARS-CoV-2 infection.
2021,
Pubmed
,
Echinobase
Dwivedi,
Anti-SARS-CoV-2 and anticoagulant properties of Pentacta pygmaea fucosylated chondroitin sulfate depend on high molecular weight structures.
2023,
Pubmed
,
Echinobase
E,
OR14I1 is a receptor for the human cytomegalovirus pentameric complex and defines viral epithelial cell tropism.
2019,
Pubmed
El Chaer,
How I treat resistant cytomegalovirus infection in hematopoietic cell transplantation recipients.
2016,
Pubmed
Farrell,
Cytomegalovirus host entry and spread.
2019,
Pubmed
Gandhi,
The structure of glycosaminoglycans and their interactions with proteins.
2008,
Pubmed
Gerna,
Human Cytomegalovirus Cell Tropism and Host Cell Receptors.
2019,
Pubmed
Griffiths,
Pathogenesis of human cytomegalovirus in the immunocompromised host.
2021,
Pubmed
Hao,
Marine glycan-based antiviral agents in clinical or preclinical trials.
2019,
Pubmed
Hirsh,
Guide to anticoagulant therapy: Heparin : a statement for healthcare professionals from the American Heart Association.
2001,
Pubmed
Hodowanec,
The Development of Therapeutics for the Treatment and Prevention of CMV Disease in the Transplant Population: A Regulatory Perspective.
2020,
Pubmed
Jin,
The structure-activity relationship of the interactions of SARS-CoV-2 spike glycoproteins with glucuronomannan and sulfated galactofucan from Saccharina japonica.
2020,
Pubmed
Kim,
Human Cytomegalovirus Requires Epidermal Growth Factor Receptor Signaling To Enter and Initiate the Early Steps in the Establishment of Latency in CD34+ Human Progenitor Cells.
2017,
Pubmed
Kim,
Fractionation of sulfated galactan from the red alga Botryocladia occidentalis separates its anticoagulant and anti-SARS-CoV-2 properties.
2022,
Pubmed
Lurain,
Antiviral drug resistance of human cytomegalovirus.
2010,
Pubmed
Malenovská,
The influence of stabilizers and rates of freezing on preserving of structurally different animal viruses during lyophilization and subsequent storage.
2014,
Pubmed
Maschke,
CNS manifestations of cytomegalovirus infections: diagnosis and treatment.
2002,
Pubmed
Mitra,
The degree of polymerization and sulfation patterns in heparan sulfate are critical determinants of cytomegalovirus entry into host cells.
2021,
Pubmed
Mitra,
Tegument Protein pp150 Sequence-Specific Peptide Blocks Cytomegalovirus Infection.
2021,
Pubmed
Nguyen,
Pathogen at the Gates: Human Cytomegalovirus Entry and Cell Tropism.
2018,
Pubmed
Oh,
Radio Frequency Heating of Washable Conductive Textiles for Bacteria and Virus Inactivation.
2022,
Pubmed
Pomin,
Current structural biology of the heparin interactome.
2015,
Pubmed
Sarrazin,
Heparan sulfate proteoglycans.
2011,
Pubmed
Shukla,
Herpesviruses and heparan sulfate: an intimate relationship in aid of viral entry.
2001,
Pubmed
Stein,
CD46 facilitates entry and dissemination of human cytomegalovirus.
2019,
Pubmed
Tandon,
Effective Inhibition of SARS-CoV-2 Entry by Heparin and Enoxaparin Derivatives.
2021,
Pubmed
Torres-Madriz,
Immunocompromised hosts: perspectives in the treatment and prophylaxis of cytomegalovirus disease in solid-organ transplant recipients.
2008,
Pubmed
Vanarsdall,
Human cytomegalovirus entry into cells.
2012,
Pubmed
Vanarsdall,
CD147 Promotes Entry of Pentamer-Expressing Human Cytomegalovirus into Epithelial and Endothelial Cells.
2018,
Pubmed
Voelker,
A Treatment Advance for Patients With Posttransplant CMV.
2022,
Pubmed
Xiao,
Neutralizing antibodies mechanism of neutralization and protective activity against HIV-1.
2002,
Pubmed
Yan,
Heparan sulfates from bat and human lung and their binding to the spike protein of SARS-CoV-2 virus.
2021,
Pubmed
Zoepfl,
Antiviral activities of four marine sulfated glycans against adenovirus and human cytomegalovirus.
2021,
Pubmed
,
Echinobase
de Paiva,
What is holding back the development of antiviral metallodrugs? A literature overview and implications for SARS-CoV-2 therapeutics and future viral outbreaks.
2020,
Pubmed