Saiwong S et al. (2023), Enzymatic Hydrolysis Optimization for Preparati...

ECB-ART-51371

Int J Mol Sci 2023 May 30;2411:. doi: 10.3390/ijms24119491.

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Enzymatic Hydrolysis Optimization for Preparation of Sea Cucumber (Holothuria scabra) Hydrolysate with an Antiproliferative Effect on the HepG2 Liver Cancer Cell Line and Antioxidant Properties.

Saiwong S , Autsavapromporn N , Siriwoharn T , Techapun C , Wangtueai S .

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The sea cucumber body wall was subjected to enzymatic hydrolysis using papain. The relationship between the enzyme concentration (1-5% w/w protein weight) and hydrolysis time (60-360 min) and the degree of hydrolysis (DH), yield, antioxidant activities, and antiproliferative activity in a HepG2 liver cancer cell line was determined. The surface response methodology showed that the optimum conditions for the enzymatic hydrolysis of sea cucumber were a hydrolysis time of 360 min and 4.3% papain. Under these conditions, a 12.1% yield, 74.52% DH, 89.74% DPPH scavenging activity, 74.92% ABTS scavenging activity, 39.42% H2O2 scavenging activity, 88.71% hydroxyl radical scavenging activity, and 9.89% HepG2 liver cancer cell viability were obtained. The hydrolysate was produced under optimum conditions and characterized in terms of its antiproliferative effect on the HepG2 liver cancer cell line.

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References [+] :

Al-Dabbagh, Antioxidant and anticancer activities of Trigonella foenum-graecum, Cassia acutifolia and Rhazya stricta. 2018, Pubmed

	Figure 1. The 3D and contour plots of the hydrolysis time (X1: min) against the concentration of papain (X2: %) for the yield ((A): %) and DH ((B): %).
	Figure 2. The 3D and contour plots of the hydrolysis time (X1: min) against the concentration of papain (X2: %) for the antioxidant activities, such as DPPH radical scavenging activity (A), ABTS radical scavenging activity (B), H2O2 scavenging activity (C), and OH· radical scavenging activity (D).
	Figure 3. The 3D and contour plots of the hydrolysis time (X1: min) against the concentration of papain (X2: %) for antiproliferative activity in the HepG2 liver cancer cell line, reported as HepG2 liver cancer cell viability (%).
	Figure 4. Plots for the significant main effects of the hydrolysis time (X1) and papain concentration (X2) on DH, yield, anticancer activity (%HepG2 cell viability), DPPH, ABTS, H2O2, and OH· radical scavenging activity. The red crosses show the center point of the factors.
	Figure 5. Plots for the interaction effects of the hydrolysis time (X1) and papain concentration (X2) on DH, yield, anticancer activity (%HepG2 cell viability), DPPH, ABTS, H2O2, and OH· radical scavenging activity.
	Figure 6. Cell viability of the HepG2 liver cancer cell line treated with the sea cucumber protein hydrolysate for 24 h at various concentrations. Different letters in the same column indicate statistical differences (p ≤ 0.05).