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Antioxidants (Basel)
2023 Jun 07;126:. doi: 10.3390/antiox12061227.
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Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus).
Li Y
,
Chen Z
,
Zhang P
,
Gao F
,
Wang J
,
Lin L
,
Zhang H
.
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At present, deep-sea enzymes are a research hotspot. In this study, a novel copper-zinc superoxide dismutase (CuZnSOD) was successfully cloned and characterized from a new species of sea cucumber Psychropotes verruciaudatus (PVCuZnSOD). The relative molecular weight of the PVCuZnSOD monomer is 15 kDa. The optimum temperature of PVCuZnSOD is 20 °C, and it maintains high activity in the range of 0-60 °C. It also has high thermal stability when incubated at 37 °C. PVCuZnSOD has a maximum activity of more than 50% in the pH range of 4-11 and a high activity at pH 11. In addition, PVCuZnSOD has strong tolerance to Ni2+, Mg2+, Ba2+, and Ca2+, and it can withstand chemical reagents, such as Tween20, TritonX-100, ethanol, glycerol, isopropanol, DMSO, urea, and GuHCl. PVCuZnSOD also shows great stability to gastrointestinal fluid compared with bovine SOD. These characteristics show that PVCuZnSOD has great application potential in medicine, food, and other products.
42006115 National Natural Science Foundation of China, 2020A1515110826 Guangdong Province Basic and Applied Basic Research Fund Project, LMM2021-5 Guangdong Key Laboratory of Marine Materia Medica, FEEL-2021-10 Fund of Guangdong Provincial Key Laboratory of Fishery Ecology and Environment
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Carillon,
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Corvo,
Superoxide dismutase enzymosomes: carrier capacity optimization, in vivo behaviour and therapeutic activity.
2015,
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Goldberg,
A single center, pilot, double-blinded, randomized, comparative, prospective clinical study to evaluate improvements in the structure and function of facial skin with tazarotene 0.1% cream alone and in combination with GliSODin(®) Skin Nutrients Advanced Anti-Aging Formula.
2014,
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Hunter,
Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue.
2002,
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Kan,
Cloning and characterization of iron-superoxide dismutase in Antarctic yeast strain Rhodotorula mucilaginosa AN5.
2017,
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Ken,
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2003,
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Kim,
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Li,
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,
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Li,
High-level expression of a sika deer (Cervus nippon) Cu/Zn superoxide dismutase in Pichia pastoris and its characterization.
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Li,
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,
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Li,
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,
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Li,
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2018,
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,
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Lin,
Cloning, purification and enzymatic characterization of recombinant human superoxide dismutase 1 (hSOD1) expressed in Escherichia coli.
2018,
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Lin,
Copper/zinc-superoxide dismutase from lemon cDNA and enzyme stability.
2002,
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Lushchak,
Free radicals, reactive oxygen species, oxidative stress and its classification.
2014,
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Mandelli,
The characterization of a thermostable and cambialistic superoxide dismutase from Thermus filiformis.
2013,
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Paravani,
Molecular analysis and bioinformatic characterization of cooper, zinc-superoxide dismutase (Cu/Zn-sod) gene of Caiman latirostris.
2020,
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Ruan,
Characterization of a novel extracellular CuZn superoxide dismutase from Rimicaris exoculata living around deep-sea hydrothermal vent.
2020,
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Sea,
Insights into the role of the unusual disulfide bond in copper-zinc superoxide dismutase.
2015,
Pubmed
Sies,
Oxidative stress: oxidants and antioxidants.
1997,
Pubmed
Sirisena,
A copper-zinc-superoxide dismutase (CuZnSOD) from redlip mullet, Liza haematocheila: Insights to its structural characteristics, immune responses, antioxidant activity, and potent antibacterial properties.
2021,
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Tamura,
MEGA11: Molecular Evolutionary Genetics Analysis Version 11.
2021,
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Teh,
Structure of Cu/Zn superoxide dismutase from the heavy-metal-tolerant yeast Cryptococcus liquefaciens strain N6.
2008,
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Wang,
Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis.
2013,
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Wang,
Purification, biochemical characterization and DNA protection against oxidative damage of a novel recombinant superoxide dismutase from psychrophilic bacterium Halomonas sp. ANT108.
2020,
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Wiseman,
Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer.
1996,
Pubmed
Zang,
Genome-wide analysis of the superoxide dismutase (SOD) gene family in Zostera marina and expression profile analysis under temperature stress.
2020,
Pubmed
Zelko,
Superoxide dismutase multigene family: a comparison of the CuZn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression.
2002,
Pubmed
Zhang,
A composite hydrogel of chitosan/heparin/poly (γ-glutamic acid) loaded with superoxide dismutase for wound healing.
2018,
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Zhu,
Cloning and characterization of a new manganese superoxide dismutase from deep-sea thermophile Geobacillus sp. EPT3.
2014,
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