Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Species-Specific Gamete Interaction during Sea Urchin Fertilization: Roles of the Egg Jelly and Vitelline Layer.
Limatola N
,
Chun JT
,
Santella L
.
???displayArticle.abstract???
In sea urchins, the sequence of the cellular and molecular events characterizing the fertilization process has been intensively studied. We have learned that to activate the egg, the fertilizing sperm must undergo morphological modifications (the acrosome reaction, AR) upon reaching the outer gelatinous layer enveloping the egg (egg jelly), which triggers the polymerization of F-actin on the sperm head to form the acrosomal process. The AR exposes bindin, an adhesive sperm protein essential for the species-specific interaction with the cognate receptor on the egg vitelline layer. To investigate the specific roles of the egg jelly and vitelline layer at fertilization of sea urchin eggs, Paracentrotus lividus eggs were incubated in acidic seawater, which removes the egg jelly, i.e., experimental conditions that should prevent the occurrence of the AR, and inseminated in the same medium. At variance with the prevailing view, our results have shown that these dejellied P. lividus eggs can still interact with sperm in acidic seawater, albeit with altered fertilization responses. In particular, the eggs deprived of the vitelline layer reacted with multiple sperm but with altered Ca2+ signals. The results have provided experimental evidence that the plasma membrane, and not the vitelline layer, is where the specific recognition between gametes occurs. The vitelline layer works in unfertilized eggs to prevent polyspermy.
Aketa,
Tryptic disruption of sperm-binding site of sea urchin egg surface.
1972, Pubmed,
Echinobase
Aketa,
Tryptic disruption of sperm-binding site of sea urchin egg surface.
1972,
Pubmed
,
Echinobase
Aketa,
When do sperm of the sea urchin, Pseudocentrotus depressus, undergo the acrosome reaction at fertilization?
1977,
Pubmed
,
Echinobase
Bleil,
Sperm-egg interactions in the mouse: sequence of events and induction of the acrosome reaction by a zona pellucida glycoprotein.
1983,
Pubmed
Chun,
Early events of fertilization in sea urchin eggs are sensitive to actin-binding organic molecules.
2014,
Pubmed
,
Echinobase
Chun,
Fertilization in Starfish and Sea Urchin: Roles of Actin.
2018,
Pubmed
,
Echinobase
Collins,
The role of calcium ions in the acrosome reaction of sea urchin sperm: regulation of exocytosis.
1977,
Pubmed
,
Echinobase
Dale,
New experimental data refuting the idea of a fast electrical block to polyspermy in sea urchin eggs.
2019,
Pubmed
,
Echinobase
Dale,
Fertilization of the starfish Astropecten aurantiacus.
1981,
Pubmed
,
Echinobase
Darszon,
Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility.
2008,
Pubmed
,
Echinobase
Epel,
Methods for revoval of the vitelline membrane of sea urchin eggs. I. Use of dithiothreitol (Cleland Reagent).
1970,
Pubmed
,
Echinobase
Epel,
The program of fertilization.
1977,
Pubmed
,
Echinobase
García-Soto,
High pH-induced acrosome reaction and Ca2+ uptake in sea urchin sperm suspended in Na+-free seawater.
1985,
Pubmed
,
Echinobase
Glabe,
Interaction of the sperm adhesive protein, bindin, with phospholipid vesicles. II. Bindin induces the fusion of mixed-phase vesicles that contain phosphatidylcholine and phosphatidylserine in vitro.
1985,
Pubmed
,
Echinobase
Glabe,
Studies on the interactions of sperm with the surface of the sea urchin egg.
1981,
Pubmed
,
Echinobase
HAGSTROM,
A method of determining the fertilization rate in sea urchins.
1954,
Pubmed
,
Echinobase
HAGSTROM,
Studies on the fertilization of jelly-free sea urchin eggs.
1956,
Pubmed
,
Echinobase
Hirohashi,
Structural requirements for species-specific induction of the sperm acrosome reaction by sea urchin egg sulfated fucan.
2002,
Pubmed
,
Echinobase
Hirohashi,
Sperm acrosome reaction: its site and role in fertilization.
2018,
Pubmed
Hoshi,
Structure of acrosome reaction-inducing substance in the jelly coat of starfish eggs: a mini review.
2012,
Pubmed
,
Echinobase
Jaffe,
Fast block to polyspermy in sea urchin eggs is electrically mediated.
1976,
Pubmed
,
Echinobase
Jin,
Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization.
2011,
Pubmed
Lallier,
Effects of concanavalin A on the development of sea urchin egg.
1972,
Pubmed
,
Echinobase
Lillie,
THE PRODUCTION OF SPERM ISO-AGGLUTININS BY OVA.
1912,
Pubmed
Limatola,
Effects of Dithiothreitol on Fertilization and Early Development in Sea Urchin.
2021,
Pubmed
,
Echinobase
Limatola,
Sodium-mediated fast electrical depolarization does not prevent polyspermic fertilization in Paracentrotus lividus eggs.
2019,
Pubmed
,
Echinobase
Limatola,
Nicotine Induces Polyspermy in Sea Urchin Eggs through a Non-Cholinergic Pathway Modulating Actin Dynamics.
2019,
Pubmed
,
Echinobase
Limatola,
Effects of Salinity and pH of Seawater on the Reproduction of the Sea Urchin Paracentrotus lividus.
2020,
Pubmed
,
Echinobase
Limatola,
Regulation of the Actin Cytoskeleton-Linked Ca2+ Signaling by Intracellular pH in Fertilized Eggs of Sea Urchin.
2022,
Pubmed
,
Echinobase
Limatola,
Fertilization and development of Arbacia lixula eggs are affected by osmolality conditions.
2021,
Pubmed
,
Echinobase
Longo,
Effects of concanavalin A on the fertilization of sea urchin eggs.
1981,
Pubmed
,
Echinobase
Metz,
Fertilization Between Closely Related Sea Urchins Is Blocked by Incompatibilities During Sperm-Egg Attachment and Early Stages of Fusion.
1994,
Pubmed
,
Echinobase
Parrington,
Flipping the switch: how a sperm activates the egg at fertilization.
2007,
Pubmed
,
Echinobase
Puppo,
Alteration of the cortical actin cytoskeleton deregulates Ca2+ signaling, monospermic fertilization, and sperm entry.
2008,
Pubmed
,
Echinobase
Runft,
Egg activation at fertilization: where it all begins.
2002,
Pubmed
Santella,
Fertilization in echinoderms.
2012,
Pubmed
,
Echinobase
Santella,
The role of the actin cytoskeleton in calcium signaling in starfish oocytes.
2008,
Pubmed
,
Echinobase
Santella,
Cellular and molecular aspects of oocyte maturation and fertilization: a perspective from the actin cytoskeleton.
2020,
Pubmed
,
Echinobase
Santella,
Calcium and actin in the saga of awakening oocytes.
2015,
Pubmed
,
Echinobase
Sawada,
Mechanisms of Sperm-Egg Interactions: What Ascidian Fertilization Research Has Taught Us.
2022,
Pubmed
Su,
Tandem mass spectrometry identifies proteins phosphorylated by cyclic AMP-dependent protein kinase when sea urchin sperm undergo the acrosome reaction.
2005,
Pubmed
,
Echinobase
Summers,
Species-specificity of acrosome reaction and primary gamete binding in echinoids.
1975,
Pubmed
,
Echinobase
Terasaki,
Actin filament translocations in sea urchin eggs.
1996,
Pubmed
,
Echinobase
Tilney,
The polymerization of actin: its role in the generation of the acrosomal process of certain echinoderm sperm.
1973,
Pubmed
,
Echinobase
Vacquier,
Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs.
1977,
Pubmed
,
Echinobase
Vacquier,
Protease activity establishes the block against polyspermy in sea urchin eggs.
1972,
Pubmed
,
Echinobase
Vacquier,
Methods for quantitating sea urchin sperm-egg binding.
1973,
Pubmed
,
Echinobase
Vacquier,
The quest for the sea urchin egg receptor for sperm.
2012,
Pubmed
,
Echinobase
Vasilev,
Contributions of suboolemmal acidic vesicles and microvilli to the intracellular Ca2+ increase in the sea urchin eggs at fertilization.
2019,
Pubmed
,
Echinobase
Veron,
Binding of concanavalin A to the surface of sea urchin eggs and its alteration upon fertilization.
1977,
Pubmed
,
Echinobase
Wessel,
Bindin is essential for fertilization in the sea urchin.
2021,
Pubmed
,
Echinobase
Wong,
Major components of a sea urchin block to polyspermy are structurally and functionally conserved.
2004,
Pubmed
,
Echinobase
Yanagimachi,
Sperm attractant in the micropyle region of fish and insect eggs.
2013,
Pubmed
Yonemura,
Wave of cortical actin polymerization in the sea urchin egg.
1987,
Pubmed
,
Echinobase
