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Cell Prolif
2023 Feb 01;562:e13351. doi: 10.1111/cpr.13351.
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Mesentery AjFGF4-AjFGFR2-ERK pathway modulates intestinal regeneration via targeting cell cycle in echinoderms.
Zeng C
,
Guo M
,
Xiang Y
,
Song M
,
Xiao K
,
Li C
.
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OBJECTIVES: The purpose of the study aims to understand the regeneration process and its cytology mechanism in economic echinoderms.
MATERIALS AND METHODS: The intestine regeneration process of Apostichopus japonicus was investigated by immunohistochemistry and the cell proliferation was detected by immunofluorescence and flow cytometry. Fibroblast growth factor 4 of A. japonicus (AjFGF4) was screened by RNA-seq analysis and validated to regulate cell proliferation by siAjFGF4 and recombinant-AjFGF4 treatment. The binding and co-localization of AjFGF4 and AjFGFR2 were verified by Co-IP, GST-pull down, and immunofluorescence. Then, the AjFGF4-AjFGFR2-ERK-cell cycle axis was examined by western blot, immunofluorescence, and flow cytometry techniques.
RESULTS: The mesentery was served as the epicenter of intestinal regeneration via activating cell proliferation and other cellular events. Mechanically, AjFGF4-mediated cell proliferation was dependent on the binding to its receptor AjFGFR2, and then triggered the conserved ERK-MAPK pathway but not JNK and p38 pathway. The activated ERK-MAPK subsequently mediated the expression of cell cycle regulatory proteins of CDK2, Cyclin A, and Cyclin B to promote cell proliferation.
CONCLUSIONS: We provide the first functional evidence that AjFGF4-AjFGFR2-ERK-cell cycle axis mediated cell proliferation was the engine for mesentery-derived intestine regeneration in echinoderms.
Ahuja,
Cardiac myocyte cell cycle control in development, disease, and regeneration.
2007, Pubmed
Ahuja,
Cardiac myocyte cell cycle control in development, disease, and regeneration.
2007,
Pubmed
Chang,
FGF9/FGFR2 increase cell proliferation by activating ERK1/2, Rb/E2F1, and cell cycle pathways in mouse Leydig tumor cells.
2018,
Pubmed
Chen,
Regulation of growth, intestinal microbiota, non-specific immune response and disease resistance of sea cucumber Apostichopus japonicus (Selenka) in biofloc systems.
2018,
Pubmed
,
Echinobase
Chen,
A unique NLRC4 receptor from echinoderms mediates Vibrio phagocytosis via rearrangement of the cytoskeleton and polymerization of F-actin.
2021,
Pubmed
,
Echinobase
Cramer,
AlphaFold2 and the future of structural biology.
2021,
Pubmed
Czarkwiani,
FGF signalling plays similar roles in development and regeneration of the skeleton in the brittle star Amphiura filiformis.
2021,
Pubmed
,
Echinobase
Forsthoefel,
An RNAi screen reveals intestinal regulators of branching morphogenesis, differentiation, and stem cell proliferation in planarians.
2012,
Pubmed
Fraguas,
EGFR signaling regulates cell proliferation, differentiation and morphogenesis during planarian regeneration and homeostasis.
2011,
Pubmed
García-Arrarás,
Visceral regeneration in holothurians.
2001,
Pubmed
,
Echinobase
García-Arrarás,
The mesentery as the epicenter for intestinal regeneration.
2019,
Pubmed
,
Echinobase
Golias,
Cell proliferation and cell cycle control: a mini review.
2004,
Pubmed
González-Rosa,
Zebrafish heart regeneration: 15 years of discoveries.
2017,
Pubmed
Haque,
MEKK4 Signaling Regulates Sensory Cell Development and Function in the Mouse Inner Ear.
2016,
Pubmed
Heber-Katz,
Cell cycle regulation and regeneration.
2013,
Pubmed
Ivankovic,
Model systems for regeneration: planarians.
2019,
Pubmed
Jimenez-Pascual,
Fibroblast Growth Factor Receptor Functions in Glioblastoma.
2019,
Pubmed
Jopling,
Zebrafish heart regeneration occurs by cardiomyocyte dedifferentiation and proliferation.
2010,
Pubmed
Kamenev,
Posterior regeneration following fission in the holothurian Cladolabes schmeltzii (Dendrochirotida: Holothuroidea).
2015,
Pubmed
,
Echinobase
Kong,
CPC: assess the protein-coding potential of transcripts using sequence features and support vector machine.
2007,
Pubmed
Langmead,
Fast gapped-read alignment with Bowtie 2.
2012,
Pubmed
Li,
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome.
2011,
Pubmed
Liu,
MAPK/ERK signalling is required for zebrafish cardiac regeneration.
2017,
Pubmed
Love,
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.
2014,
Pubmed
Lv,
Role of FGF10/FGFR2b Signaling in Mouse Digestive Tract Development, Repair and Regeneration Following Injury.
2019,
Pubmed
Maddaluno,
Fibroblast growth factors: key players in regeneration and tissue repair.
2017,
Pubmed
Mashanov,
Expression of pluripotency factors in echinoderm regeneration.
2015,
Pubmed
,
Echinobase
Nowoshilow,
The axolotl genome and the evolution of key tissue formation regulators.
2018,
Pubmed
Ohori,
Identification of a selective ERK inhibitor and structural determination of the inhibitor-ERK2 complex.
2005,
Pubmed
Okada,
Regeneration of the digestive tract of an anterior-eviscerating sea cucumber, Eupentacta quinquesemita, and the involvement of mesenchymal-epithelial transition in digestive tube formation.
2019,
Pubmed
,
Echinobase
Oulion,
Evolution of the FGF Gene Family.
2012,
Pubmed
Owlarn,
Generic wound signals initiate regeneration in missing-tissue contexts.
2017,
Pubmed
Pierce,
ZDOCK server: interactive docking prediction of protein-protein complexes and symmetric multimers.
2014,
Pubmed
Porrello,
Transient regenerative potential of the neonatal mouse heart.
2011,
Pubmed
Quispe-Parra,
A roadmap for intestinal regeneration.
2021,
Pubmed
,
Echinobase
Ricci,
Wound-induced cell proliferation during animal regeneration.
2018,
Pubmed
Saera-Vila,
Fgf regulates dedifferentiation during skeletal muscle regeneration in adult zebrafish.
2016,
Pubmed
Stoick-Cooper,
Advances in signaling in vertebrate regeneration as a prelude to regenerative medicine.
2007,
Pubmed
Sun,
ROS-mediated BNIP3-dependent mitophagy promotes coelomocyte survival in Apostichopus japonicus in response to Vibrio splendidus infection.
2022,
Pubmed
,
Echinobase
Tanaka,
The Molecular and Cellular Choreography of Appendage Regeneration.
2016,
Pubmed
Tanaka,
Regenerating tissues.
2018,
Pubmed
Wen,
MAPK/ERK Pathway as a Central Regulator in Vertebrate Organ Regeneration.
2022,
Pubmed
Zhang,
The sea cucumber genome provides insights into morphological evolution and visceral regeneration.
2017,
Pubmed
,
Echinobase