Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
eIF4B mRNA Translation Contributes to Cleavage Dynamics in Early Sea Urchin Embryos.
Pontheaux F
,
Boulben S
,
Chassé H
,
Boutet A
,
Roch F
,
Morales J
,
Cormier P
.
???displayArticle.abstract???
During the first steps of sea urchin development, fertilization elicits a marked increase in protein synthesis essential for subsequent cell divisions. While the translation of mitotic cyclin mRNAs is crucial, we hypothesized that additional mRNAs must be translated to finely regulate the onset into mitosis. One of the maternal mRNAs recruited onto active polysomes at this stage codes for the initiation factor eIF4B. Here, we show that the sea urchin eIF4B orthologs present the four specific domains essential for eIF4B function and that Paracentrotus lividus eIF4B copurifies with eIF4E in a heterologous system. In addition, we investigated the role of eIF4B mRNA de novo translation during the two first embryonic divisions of two species, P. lividus and Sphaerechinus granularis. Our results show that injection of a morpholino directed against eIF4B mRNA results in a downregulation of translational activity and delays cell division in these two echinoids. Conversely, injection of an mRNA encoding for P. lividus eIF4B stimulates translation and significantly accelerates cleavage rates. Taken together, our findings suggest that eIF4B mRNA de novo translation participates in a conserved regulatory loop that contributes to orchestrating protein synthesis and modulates cell division rhythm during early sea urchin development.
Altmann,
A Saccharomyces cerevisiae homologue of mammalian translation initiation factor 4B contributes to RNA helicase activity.
1993, Pubmed
Altmann,
A Saccharomyces cerevisiae homologue of mammalian translation initiation factor 4B contributes to RNA helicase activity.
1993,
Pubmed
Andreou,
eIF4B stimulates eIF4A ATPase and unwinding activities by direct interaction through its 7-repeats region.
2017,
Pubmed
Bellé,
Model of cap-dependent translation initiation in sea urchin: a step towards the eukaryotic translation regulation network.
2010,
Pubmed
,
Echinobase
Benne,
The mechanism of action of protein synthesis initiation factors from rabbit reticulocytes.
1978,
Pubmed
Chassé,
In vivo analysis of protein translation activity in sea urchin eggs and embryos.
2019,
Pubmed
,
Echinobase
Chassé,
Translatome analysis at the egg-to-embryo transition in sea urchin.
2018,
Pubmed
,
Echinobase
Chassé,
Analysis of translation using polysome profiling.
2017,
Pubmed
,
Echinobase
Chassé,
Translational Control of Canonical and Non-Canonical Translation Initiation Factors at the Sea Urchin Egg to Embryo Transition.
2019,
Pubmed
,
Echinobase
Chassé,
Cyclin B Translation Depends on mTOR Activity after Fertilization in Sea Urchin Embryos.
2016,
Pubmed
,
Echinobase
Coppolecchia,
A new yeast translation initiation factor suppresses a mutation in the eIF-4A RNA helicase.
1993,
Pubmed
Cormier,
eIF4E association with 4E-BP decreases rapidly following fertilization in sea urchin.
2001,
Pubmed
,
Echinobase
Costache,
Dephosphorylation of eIF2α is essential for protein synthesis increase and cell cycle progression after sea urchin fertilization.
2012,
Pubmed
,
Echinobase
Daga,
Translational control of the cdc25 cell cycle phosphatase: a molecular mechanism coupling mitosis to cell growth.
1999,
Pubmed
De Nadai,
Effect of wortmannin, an inhibitor of phosphatidylinositol 3-kinase, on the first mitotic divisions of the fertilized sea urchin egg.
1998,
Pubmed
,
Echinobase
Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed
,
Echinobase
Gray,
Poly(A)-binding proteins and mRNA localization: who rules the roost?
2015,
Pubmed
Groisman,
Translational control of the embryonic cell cycle.
2002,
Pubmed
GROSS,
Effects of actinomycin D on macromolecule synthesis and early development in sea urchin eggs.
1963,
Pubmed
,
Echinobase
Hernández,
Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation.
2004,
Pubmed
Holz,
mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events.
2005,
Pubmed
Jacobs,
Drosophila Cyclin B3 is required for female fertility and is dispensable for mitosis like Cyclin B.
1998,
Pubmed
Jones,
The rapid generation of mutation data matrices from protein sequences.
1992,
Pubmed
Kang,
Punctuated cyclin synthesis drives early embryonic cell cycle oscillations.
2012,
Pubmed
Keiper,
Protein synthesis initiation factor 4G.
1999,
Pubmed
Kronja,
Translational regulation of the cell cycle: when, where, how and why?
2011,
Pubmed
Láruson,
Rates and relations of mitochondrial genome evolution across the Echinoidea, with special focus on the superfamily Odontophora.
2017,
Pubmed
,
Echinobase
Laurent,
Modelization of the regulation of protein synthesis following fertilization in sea urchin shows requirement of two processes: a destabilization of eIF4E:4E-BP complex and a great stimulation of the 4E-BP-degradation mechanism, both rapamycin-sensitive.
2014,
Pubmed
,
Echinobase
Legagneux,
Substrate-specific regulation of RNA deadenylation in Xenopus embryo and activated egg extracts.
1995,
Pubmed
Lehner,
The roles of Drosophila cyclins A and B in mitotic control.
1990,
Pubmed
Letunic,
SMART: recent updates, new developments and status in 2020.
2021,
Pubmed
Malumbres,
Cyclin-dependent kinases.
2014,
Pubmed
Metz,
Eukaryotic initiation factor 4B from wheat and Arabidopsis thaliana is a member of a multigene family.
1999,
Pubmed
Milburn,
Cloning and expression of eukaryotic initiation factor 4B cDNA: sequence determination identifies a common RNA recognition motif.
1990,
Pubmed
Morales,
Translational control genes in the sea urchin genome.
2006,
Pubmed
,
Echinobase
Moreau,
Cdk2 activity is dispensable for the onset of DNA replication during the first mitotic cycles of the sea urchin early embryo.
1998,
Pubmed
,
Echinobase
Moundoyi,
Toward Multiscale Modeling of Molecular and Biochemical Events Occurring at Fertilization Time in Sea Urchins.
2018,
Pubmed
,
Echinobase
Naranda,
Two structural domains of initiation factor eIF-4B are involved in binding to RNA.
1994,
Pubmed
Nigg,
Mitotic kinases as regulators of cell division and its checkpoints.
2001,
Pubmed
Oulhen,
After fertilization of sea urchin eggs, eIF4G is post-translationally modified and associated with the cap-binding protein eIF4E.
2007,
Pubmed
,
Echinobase
Panda,
Polysome Fractionation to Analyze mRNA Distribution Profiles.
2017,
Pubmed
Parsyan,
mRNA helicases: the tacticians of translational control.
2011,
Pubmed
Pelletier,
The Organizing Principles of Eukaryotic Ribosome Recruitment.
2019,
Pubmed
Philippe,
Global analysis of LARP1 translation targets reveals tunable and dynamic features of 5' TOP motifs.
2020,
Pubmed
Picard,
Model of the delayed translation of cyclin B maternal mRNA after sea urchin fertilization.
2016,
Pubmed
,
Echinobase
Richter-Cook,
Purification and characterization of a new eukaryotic protein translation factor. Eukaryotic initiation factor 4H.
1998,
Pubmed
Rogers,
Modulation of the helicase activity of eIF4A by eIF4B, eIF4H, and eIF4F.
2001,
Pubmed
Rozovsky,
Interactions between eIF4AI and its accessory factors eIF4B and eIF4H.
2008,
Pubmed
Salaün,
eIF4E/4E-BP dissociation and 4E-BP degradation in the first mitotic division of the sea urchin embryo.
2003,
Pubmed
,
Echinobase
Schindelin,
Fiji: an open-source platform for biological-image analysis.
2012,
Pubmed
Shahbazian,
The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity.
2006,
Pubmed
Shahbazian,
Control of cell survival and proliferation by mammalian eukaryotic initiation factor 4B.
2010,
Pubmed
Spieth,
Effect of 3'-deoxyadenosine (cordycepin) on the early development of the sand dollar, Dendraster excentricus.
1980,
Pubmed
,
Echinobase
Stepicheva,
High throughput microinjections of sea urchin zygotes.
2014,
Pubmed
,
Echinobase
Thoreen,
A unifying model for mTORC1-mediated regulation of mRNA translation.
2012,
Pubmed
van Gorp,
AGC kinases regulate phosphorylation and activation of eukaryotic translation initiation factor 4B.
2009,
Pubmed
Voronina,
Cyclin B synthesis is required for sea urchin oocyte maturation.
2003,
Pubmed
,
Echinobase
Wagenaar,
The timing of synthesis of proteins required for mitosis in the cell cycle of the sea urchin embryo.
1983,
Pubmed
,
Echinobase
Wilt,
The dynamics of maternal poly(A)-containing mRNA in fertilized sea urchin eggs.
1977,
Pubmed
,
Echinobase
