Metwally RA , El-Sersy NA , El Sikaily A , Sabry SA , Ghozlan HA .

	Figure 1. Kocuria sp. RAM1 with orange pigmentation isolated from Bohadschia graeffei, Marsa Alam, Red Sea, Egypt.
	Figure 2. Phylogenetic tree of Kocuria sp. RAM1 estimated using maximum likelihood (ML). Kocuria sp. RAM1 was indicated by a bold style font. GenBank sequence accession numbers are indicated in parentheses after the strain names. The evolutionary analysis was conducted in the software package MEGA 11.
	Figure 3. Kocuria sp. RAM1 carotenoids. (A) Carotenoids dissolved in petroleum ether. (B) Thin-Layer Chromatography (TLC) [solvent system = chloroform: methanol (6:1; v/v)]. (C) UV–Vis spectra of the 3 purified pigment spots obtained from TLC.
	Figure 4. 1H NMR spectra (A) and corresponding HPLC-QTOF-HRMS analysis (B) of the 3 Kocuria sp. RAM1 purified pigments (spot 1 = bisanhydrobacterioruberin, spot 2 = trisanhydrobacterioruberin and spot 3 = 3,4,3ʹ,4ʹ-tetrahydrospirilloxanthin).
	Figure 5. Plackett–Burman design for Kocuria sp. RAM1 carotenoids optimization. (A) Carotenoids yield predicted value versus experimental value. (B) PBD factors that influence carotenoids yield. The orange and blue bars are corresponding to the positive and negative effect, respectively. A: Peptone (g/l), B: Yeast extract (g/l), C: Beef extract (g/l), D: NaCl (g/l), E: Glucose (g/l), F: MgSO4 (g/l), G: pH, H: Temperature, J: Agitation (rpm), K: Inoculum size (%), L: Incubation period (h).
	Figure 6. RSM plots of Kocuria sp. RAM1 carotenoids. (A) Temperature and peptone interaction, (B) Agitation and peptone interaction, (C) Inoculum size and peptone interaction, (D) Agitation and temperature interaction, (E) Inoculum size and temperature interaction, (F) Inoculum size and agitation interaction.
	Figure 7. Anti-inflammatory activity of Kocuria sp. RAM1 carotenoids. The letters a, b, c, d, e and f represent significant differences among different homogeneous subsets identified by the post hoc test for p < 0.05. The data represent the mean ± SD (n = 3).
	Figure 8. (A) Antioxidant activity of Kocuria sp. RAM1 carotenoids. The letters from a to j represent significant differences among different homogeneous subsets identified by the post hoc test for p < 0.05. (B) IC50 estimation. The data represent the mean ± SD (n = 3).
	Figure 9. MNTD estimation of Kocuria sp. RAM1 carotenoids. (A) Cytotoxicity on normal Vero cells. The letters a, b and c represent significant differences among different homogeneous subsets identified by the post hoc test for p < 0.05. (**)Significant at p < 0.01; n.s non-significant. (B) IC50 estimation. The data represent the mean ± SD (n = 3).
	Figure 10. Wound closure percentage using Kocuria sp. RAM1 carotenoids of (A) human skin fibroblasts (HSF) and (B) oral epithelial cells (OEC) at different time intervals (0, 24, 48 and 72 h). Cells were injured by a straight-line scratch across the monolayer, and photographs were captured at different time intervals (0, 24, 48 and 72 h). The data represent the mean ± SD (n = 3).
	Figure 11. Effect of Kocuria sp. RAM1 carotenoids on HSV-1 infectivity. The data represent the mean ± SD (n = 3).
	Figure 12. Cytotoxicity of Kocuria sp. RAM1 carotenoids against MCF-7, Caco-2 and Hela cell lines. The letters a, b, c and d represent significant differences among different homogeneous subsets identified by the post hoc test for p < 0.05. (**)Significant at p < 0.01; n.s non-significant. The data represent the mean ± SD (n = 3).
	Figure 13. Imaging and IC10 and IC50 estimations of (A) MCF-7, (B) Caco-2 and (C) Hela cancer cell lines treated with different concentrations of Kocuria sp. RAM1 carotenoids by the in vitro cytotoxicity MTT test. GraphPad Prism 9 was used for plots.
	Figure 13. (A) In vitro α-glucosidase inhibitory effect of Kocuria sp. RAM1 carotenoids vs. acarbose (positive control) at different concentrations. (B) IC50 estimations. The data represent the mean ± SD (n = 3).

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