Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Int J Mol Sci
2022 Nov 17;2322:. doi: 10.3390/ijms232214238.
Show Gene links
Show Anatomy links
Vanadium Modulates Proteolytic Activities and MMP-14-Like Levels during Paracentrotus lividus Embryogenesis.
Chiarelli R
,
Martino C
,
Scudiero R
,
Geraci F
.
???displayArticle.abstract???
The increasing industrial use of vanadium (V), as well as its recent medical use in various pathologies has intensified its environmental release, making it an emerging pollutant. The sea urchin embryo has long been used to study the effects induced by metals, including V. In this study we used an integrated approach that correlates the biological effects on embryo development with proteolytic activities of gelatinases that could better reflect any metal-induced imbalances. V-exposure caused morphological/morphometric aberrations, mainly concerning the correct distribution of embryonic cells, the development of the skeleton, and the embryo volume. Moreover, V induced a concentration change in all the gelatinases expressed during embryo development and a reduction in their total proteolytic activity. The presence of three MMP-like gelatinases (MMP-2, -9, and -14) was also demonstrated and their levels depended on V-concentration. In particular, the MMP-14-like protein modified its expression level during embryo development in a time- and dose-dependent manner. This enzyme also showed a specific localization on filopodia, suggesting that primary mesenchyme cells (PMCs) could be responsible for its synthesis. In conclusion, these results indicate that an integrated study among morphology/morphometry, proteolytic activity, and MMP-14 expression constitutes an important response profile to V-action.
Aiken,
The direct measurement of embryogenic volume and nucleo-cytoplasmic ratio during mouse pre-implantation development.
2004, Pubmed
Aiken,
The direct measurement of embryogenic volume and nucleo-cytoplasmic ratio during mouse pre-implantation development.
2004,
Pubmed
Angerer,
Sea urchin metalloproteases: a genomic survey of the BMP-1/tolloid-like, MMP and ADAM families.
2006,
Pubmed
,
Echinobase
Bonaventura,
Nickel toxicity in P. lividus embryos: Dose dependent effects and gene expression analysis.
2018,
Pubmed
,
Echinobase
Cappello,
Alteration of neurotransmission and skeletogenesis in sea urchin Arbacia lixula embryos exposed to copper oxide nanoparticles.
2017,
Pubmed
,
Echinobase
Chiarelli,
Toxic effects induced by vanadium on sea urchin embryos.
2021,
Pubmed
,
Echinobase
Chiarelli,
Sea urchin embryos as a model system for studying autophagy induced by cadmium stress.
2011,
Pubmed
,
Echinobase
Chiarelli,
Heavy metals and metalloids as autophagy inducing agents: focus on cadmium and arsenic.
2012,
Pubmed
,
Echinobase
Chiarelli,
Autophagy as a defense strategy against stress: focus on Paracentrotus lividus sea urchin embryos exposed to cadmium.
2016,
Pubmed
,
Echinobase
Chiarelli,
Vanadium Toxicity Monitored by Fertilization Outcomes and Metal Related Proteolytic Activities in Paracentrotus lividus Embryos.
2022,
Pubmed
,
Echinobase
Chiarelli,
Toxicity of Vanadium during Development of Sea Urchin Embryos: Bioaccumulation, Calcium Depletion, ERK Modulation and Cell-Selective Apoptosis.
2022,
Pubmed
,
Echinobase
Chiarelli,
Cadmium stress effects indicating marine pollution in different species of sea urchin employed as environmental bioindicators.
2019,
Pubmed
,
Echinobase
Colín-Barenque,
Matrix metalloproteinases 2 and 9 in central nervous system and their modification after vanadium inhalation.
2008,
Pubmed
Cunha,
Mussels as bioindicators of diclofenac contamination in coastal environments.
2017,
Pubmed
Davidson,
The sea urchin genome: where will it lead us?
2006,
Pubmed
,
Echinobase
Gambardella,
Review: Morphofunctional and biochemical markers of stress in sea urchin life stages exposed to engineered nanoparticles.
2016,
Pubmed
,
Echinobase
Garmon,
MMP14 Regulates Cranial Neural Crest Epithelial-to-Mesenchymal Transition and Migration.
2018,
Pubmed
Geraci,
Nickel, lead, and cadmium induce differential cellular responses in sea urchin embryos by activating the synthesis of different HSP70s.
2004,
Pubmed
,
Echinobase
Giannetto,
Copper oxide nanoparticles induce the transcriptional modulation of oxidative stress-related genes in Arbacia lixula embryos.
2018,
Pubmed
,
Echinobase
Gifford,
MT1-MMP-dependent cell migration: proteolytic and non-proteolytic mechanisms.
2019,
Pubmed
Hamdoun,
Embryo stability and vulnerability in an always changing world.
2007,
Pubmed
Hohensinner,
Statin treatment reduces matrix degradation capacity of proinflammatory polarized macrophages.
2018,
Pubmed
Holmbeck,
MT1-MMP-deficient mice develop dwarfism, osteopenia, arthritis, and connective tissue disease due to inadequate collagen turnover.
1999,
Pubmed
Ingersoll,
Matrix metalloproteinase inhibitors disrupt spicule formation by primary mesenchyme cells in the sea urchin embryo.
1998,
Pubmed
,
Echinobase
Ingersoll,
Ultrastructural localization of spicule matrix proteins in normal and metalloproteinase inhibitor-treated sea urchin primary mesenchyme cells.
2003,
Pubmed
,
Echinobase
Itoh,
Membrane-type matrix metalloproteinases: Their functions and regulations.
2015,
Pubmed
Jia,
Smad signaling in the neural crest regulates cardiac outflow tract remodeling through cell autonomous and non-cell autonomous effects.
2007,
Pubmed
Li,
Bis(α-furancarboxylato)oxovanadium(IV) exerts durable antidiabetic effects and suppresses matrix metalloproteinase-2 activity in spontaneous type 2 diabetic KKAy mice.
2013,
Pubmed
Maisano,
Developmental abnormalities and neurotoxicological effects of CuO NPs on the black sea urchin Arbacia lixula by embryotoxicity assay.
2015,
Pubmed
,
Echinobase
Malemud,
Matrix metalloproteinases: role in skeletal development and growth plate disorders.
2006,
Pubmed
Martino,
Induction of skeletal abnormalities and autophagy in Paracentrotus lividus sea urchin embryos exposed to gadolinium.
2017,
Pubmed
,
Echinobase
Martino,
Gadolinium perturbs expression of skeletogenic genes, calcium uptake and larval development in phylogenetically distant sea urchin species.
2018,
Pubmed
,
Echinobase
Martino,
Interactive effects of increased temperature and gadolinium pollution in Paracentrotus lividus sea urchin embryos: a climate change perspective.
2021,
Pubmed
,
Echinobase
Mauro,
Human Drug Pollution in the Aquatic System: The Biochemical Responses of Danio rerio Adults.
2021,
Pubmed
Mori,
CD44 directs membrane-type 1 matrix metalloproteinase to lamellipodia by associating with its hemopexin-like domain.
2002,
Pubmed
Muñoz-Sáez,
Dynamic Expression of Membrane Type 1-Matrix Metalloproteinase (Mt1-mmp/Mmp14) in the Mouse Embryo.
2021,
Pubmed
Müller,
Changes of cell and embryo volume during development of vitelline membrane-free sea urchin embryos.
1972,
Pubmed
,
Echinobase
Nomura,
Sea urchin hatching enzyme (envelysin): cDNA cloning and deprivation of protein substrate specificity by autolytic degradation.
1997,
Pubmed
,
Echinobase
Oblander,
Distinctive functions of membrane type 1 matrix-metalloprotease (MT1-MMP or MMP-14) in lung and submandibular gland development are independent of its role in pro-MMP-2 activation.
2005,
Pubmed
Oliver,
Vanadate, an inhibitor of stromelysin and collagenase expression, suppresses collagen induced arthritis.
2007,
Pubmed
Petanidis,
Novel ternary vanadium-betaine-peroxido species suppresses H-ras and matrix metalloproteinase-2 expression by increasing reactive oxygen species-mediated apoptosis in cancer cells.
2013,
Pubmed
Pinsino,
Manganese interferes with calcium, perturbs ERK signaling, and produces embryos with no skeleton.
2011,
Pubmed
,
Echinobase
Rana,
Is there a Role for Sodium Orthovanadate in the Treatment of Diabetes?
2019,
Pubmed
Rehder,
The role of vanadium in biology.
2015,
Pubmed
Roe,
Inhibitors of metalloendoproteases block spiculogenesis in sea urchin primary mesenchyme cells.
1989,
Pubmed
,
Echinobase
Rolo,
Measurement of embryo volume at 7-10 weeks' gestation by 3D-sonography.
2009,
Pubmed
Russo,
Stress to cadmium monitored by metallothionein gene induction in Paracentrotus lividus embryos.
2003,
Pubmed
,
Echinobase
Sang,
Computational sequence analysis of matrix metalloproteinases.
1996,
Pubmed
Shi,
Membrane-type MMPs enable extracellular matrix permissiveness and mesenchymal cell proliferation during embryogenesis.
2008,
Pubmed
Sodergren,
The genome of the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Vethaak,
Toxicity profiling of marine surface sediments: A case study using rapid screening bioassays of exhaustive total extracts, elutriates and passive sampler extracts.
2017,
Pubmed
,
Echinobase
Williams,
VANGL2 regulates membrane trafficking of MMP14 to control cell polarity and migration.
2012,
Pubmed
Xu,
Multiple essential MT1-MMP functions in tooth root formation, dentinogenesis, and tooth eruption.
2016,
Pubmed
Zhou,
Impaired endochondral ossification and angiogenesis in mice deficient in membrane-type matrix metalloproteinase I.
2000,
Pubmed
Zotin,
[The rate of oxygen consumption during embryogenesis of Lymnaea stagnalis (Gastropoda)].
2006,
Pubmed
Ågren,
Matrix Metalloproteinases: How Much Can They Do?
2020,
Pubmed
Ścibior,
Vanadium: Risks and possible benefits in the light of a comprehensive overview of its pharmacotoxicological mechanisms and multi-applications with a summary of further research trends.
2020,
Pubmed
