Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules.
Hotta T
,
McAlear TS
,
Yue Y
,
Higaki T
,
Haynes SE
,
Nesvizhskii AI
,
Sept D
,
Verhey KJ
,
Bechstedt S
,
Ohi R
.
???displayArticle.abstract???
Tubulin post-translational modifications (PTMs) alter microtubule properties by affecting the binding of microtubule-associated proteins (MAPs). Microtubule detyrosination, which occurs by proteolytic removal of the C-terminal tyrosine from ɑ-tubulin, generates the oldest known tubulin PTM, but we lack comprehensive knowledge of MAPs that are regulated by this PTM. We developed a screening pipeline to identify proteins that discriminate between Y- and ΔY-microtubules and found that echinoderm microtubule-associated protein-like 2 (EML2) preferentially interacts with Y-microtubules. This activity depends on a Y-microtubule interaction motif built from WD40 repeats. We show that EML2 tracks the tips of shortening microtubules, a behavior not previously seen among human MAPs in vivo, and influences dynamics to increase microtubule stability. Our screening pipeline is readily adapted to identify proteins that specifically recognize a wide range of microtubule PTMs.
Adib,
Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression.
2019, Pubmed
Adib,
Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression.
2019,
Pubmed
Aillaud,
Vasohibins/SVBP are tubulin carboxypeptidases (TCPs) that regulate neuron differentiation.
2017,
Pubmed
Akhmanova,
Tracking the ends: a dynamic protein network controls the fate of microtubule tips.
2008,
Pubmed
Arnal,
CLIP-170/tubulin-curved oligomers coassemble at microtubule ends and promote rescues.
2004,
Pubmed
Backer,
CSAP localizes to polyglutamylated microtubules and promotes proper cilia function and zebrafish development.
2012,
Pubmed
Baker,
Electrostatics of nanosystems: application to microtubules and the ribosome.
2001,
Pubmed
Barisic,
Mitosis. Microtubule detyrosination guides chromosomes during mitosis.
2015,
Pubmed
Best,
Optimization of the additive CHARMM all-atom protein force field targeting improved sampling of the backbone φ, ψ and side-chain χ(1) and χ(2) dihedral angles.
2012,
Pubmed
Cai,
Single molecule imaging reveals differences in microtubule track selection between Kinesin motors.
2009,
Pubmed
Chen,
α-tubulin tail modifications regulate microtubule stability through selective effector recruitment, not changes in intrinsic polymer dynamics.
2021,
Pubmed
da Veiga Leprevost,
Philosopher: a versatile toolkit for shotgun proteomics data analysis.
2020,
Pubmed
Drechsler,
Multivalent electrostatic microtubule interactions of synthetic peptides are sufficient to mimic advanced MAP-like behavior.
2019,
Pubmed
Du,
The kinesin-8 Kif18A dampens microtubule plus-end dynamics.
2010,
Pubmed
Dunn,
Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells.
2008,
Pubmed
Eichenmuller,
The human EMAP-like protein-70 (ELP70) is a microtubule destabilizer that localizes to the mitotic apparatus.
2002,
Pubmed
,
Echinobase
Fry,
EML proteins in microtubule regulation and human disease.
2016,
Pubmed
,
Echinobase
Fujii,
PA tag: a versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin.
2014,
Pubmed
Gayek,
Kinetochore-microtubule stability governs the metaphase requirement for Eg5.
2014,
Pubmed
Gell,
Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy.
2010,
Pubmed
Hotta,
Affinity Purification and Characterization of Functional Tubulin from Cell Suspension Cultures of Arabidopsis and Tobacco.
2016,
Pubmed
Hotta,
Parthenolide Destabilizes Microtubules by Covalently Modifying Tubulin.
2021,
Pubmed
Huang,
CHARMM36 all-atom additive protein force field: validation based on comparison to NMR data.
2013,
Pubmed
Humphrey,
VMD: visual molecular dynamics.
1996,
Pubmed
Hyman,
Preparation of modified tubulins.
1991,
Pubmed
Jijumon,
Lysate-based pipeline to characterize microtubule-associated proteins uncovers unique microtubule behaviours.
2022,
Pubmed
Kannan,
WD40-repeat 47, a microtubule-associated protein, is essential for brain development and autophagy.
2017,
Pubmed
Kett,
LRRK2 Parkinson disease mutations enhance its microtubule association.
2012,
Pubmed
Khandelia,
Streamlined platform for short hairpin RNA interference and transgenesis in cultured mammalian cells.
2011,
Pubmed
Kim,
WD40-Repeat Proteins in Ciliopathies and Congenital Disorders of Endocrine System.
2020,
Pubmed
Kong,
MSFragger: ultrafast and comprehensive peptide identification in mass spectrometry-based proteomics.
2017,
Pubmed
Konishi,
Tubulin tyrosination navigates the kinesin-1 motor domain to axons.
2009,
Pubmed
Kreitzer,
Detyrosination of tubulin regulates the interaction of intermediate filaments with microtubules in vivo via a kinesin-dependent mechanism.
1999,
Pubmed
Kuo,
Spastin is a dual-function enzyme that severs microtubules and promotes their regrowth to increase the number and mass of microtubules.
2019,
Pubmed
Lacroix,
Tubulin polyglutamylation stimulates spastin-mediated microtubule severing.
2010,
Pubmed
Landskron,
Posttranslational modification of microtubules by the MATCAP detyrosinase.
2022,
Pubmed
Lepley,
Sequence and expression patterns of a human EMAP-related protein-2 (HuEMAP-2).
1999,
Pubmed
,
Echinobase
Miller,
Golgi-derived CLASP-dependent microtubules control Golgi organization and polarized trafficking in motile cells.
2009,
Pubmed
Miller,
Methods in tubulin proteomics.
2010,
Pubmed
Mishima,
Structural basis for tubulin recognition by cytoplasmic linker protein 170 and its autoinhibition.
2007,
Pubmed
Mohan,
Detyrosinated microtubules spatially constrain lysosomes facilitating lysosome-autophagosome fusion.
2019,
Pubmed
Nieuwenhuis,
Vasohibins encode tubulin detyrosinating activity.
2017,
Pubmed
Peris,
Motor-dependent microtubule disassembly driven by tubulin tyrosination.
2009,
Pubmed
Phillips,
Scalable molecular dynamics on CPU and GPU architectures with NAMD.
2020,
Pubmed
Richards,
Crystal structure of EML1 reveals the basis for Hsp90 dependence of oncogenic EML4-ALK by disruption of an atypical β-propeller domain.
2014,
Pubmed
,
Echinobase
Richards,
Microtubule association of EML proteins and the EML4-ALK variant 3 oncoprotein require an N-terminal trimerization domain.
2015,
Pubmed
,
Echinobase
Robison,
Detyrosinated microtubules buckle and bear load in contracting cardiomyocytes.
2016,
Pubmed
Roll-Mecak,
The Tubulin Code in Microtubule Dynamics and Information Encoding.
2020,
Pubmed
Schmidt,
The kinetochore-bound Ska1 complex tracks depolymerizing microtubules and binds to curved protofilaments.
2012,
Pubmed
Schröder,
Purification of brain tubulin-tyrosine ligase by biochemical and immunological methods.
1985,
Pubmed
Stauffer,
EzColocalization: An ImageJ plugin for visualizing and measuring colocalization in cells and organisms.
2018,
Pubmed
Steinmetz,
Capturing protein tails by CAP-Gly domains.
2008,
Pubmed
Sturgill,
Kinesin-12 differentially affects spindle assembly depending on its microtubule substrate.
2013,
Pubmed
Suprenant,
EMAP, an echinoderm microtubule-associated protein found in microtubule-ribosome complexes.
1993,
Pubmed
,
Echinobase
Takatani,
Directional cell expansion requires NIMA-related kinase 6 (NEK6)-mediated cortical microtubule destabilization.
2017,
Pubmed
Thompson,
Tandem mass tags: a novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS.
2003,
Pubmed
Trott,
AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading.
2010,
Pubmed
van Riel,
Kinesin-4 KIF21B is a potent microtubule pausing factor.
2017,
Pubmed
Walker,
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
1988,
Pubmed
Weingarten,
A protein factor essential for microtubule assembly.
1975,
Pubmed
Weisbrich,
Structure-function relationship of CAP-Gly domains.
2007,
Pubmed
Westermann,
The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends.
2006,
Pubmed
Widlund,
One-step purification of assembly-competent tubulin from diverse eukaryotic sources.
2012,
Pubmed
Yao,
Arabidopsis SPIRAL2 promotes uninterrupted microtubule growth by suppressing the pause state of microtubule dynamics.
2008,
Pubmed
