Karetin YA (2021), Morphometry of cellular behavior of coelomocyte...

ECB-ART-50635

PeerJ 2021 Apr 06;9:e12514. doi: 10.7717/peerj.12514.

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Morphometry of cellular behavior of coelomocytes from starfish Asterias amurensis.

Karetin YA .

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A comprehensive statistical analysis using a wide range of linear and non-linear morphological parameters enabled identification of the main stages in the in vitro dynamics of cell behavior of immune cells of the marine invertebrate Asterias amurensis (Echinodermata, Asteroidea). Three stages may be distinguished in the cell behavior, which are characterized by the differences in complexity of the cell boundary microsculpture as well as by the size and asymmetry of the cell and convex hull of the cell. The first stage (5 min after placing cells onto a substrate) is characterized by more complex cell morphology and an increase in the process number and spreading area. The second stage (15 min) is characterized by simplification of cell morphology, retraction of some processes, and rounding of cells upon continued cell spreading. At the third stage (60 min), new large processes with rounded contours emerge due to partial retraction of the flattened cell surface. Each stage is characterized by statistically significant differences in several linear and nonlinear parameters of the external morphology for all cell types.

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References [+] :

Bossche, Epithelial origin of starfish coelomocytes. 1976, Pubmed, Echinobase

	Figure 1. Silhouette images of cells at different stages of cultivation: (A) 2 min (group 1); (B) 5 min (group 2); (C) 15 min (group 3); (D) 60 min (group 4).
	Figure 2. Different trends in changes in parameter values during in vitro culturing of coelomocytes from A. amurensis.(A) Area; (B) circularity; (C) Feret’s diameter; (D) mean local connected fractal dimension of contour images of cells; (E) in50/out50.
	Figure 3. Convex hull of cell.
	Figure 4. 1/2half and in50/out50.calculation. 1/2half = A/B , where A, B –two segments of the cell lying in different halves of the bounding circle . in50/out50 = C/D, where C and D are the parts of the cell located in the inner and outer part of the.
	Figure 5. Box-counting fractal dimension calculation.See the description of the method in the text.
	Figure 6. Cluster diagrams for 4 time groups, plotted using Area, Circ, Feret, in50/out50, and LE parameters (A) 2 min; (B) 5 min; (C) 15 min; (D) 60 min. Ward‘s method, Euclidean distance measure.
	Figure 7. Silhouette images of type I–III cells from all time groups.
	Figure 8. Plots of means and 95% confidence intervals for parameters of identified cell types: (A) area, (B) Feret’s diameter, (C) perimeter, (D) Hull’s perimeter, (E) roundness.
	Figure 9. Cell morphology corresponding to the different values of outMeanD and outMeanLFD parameters.