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Biology (Basel)
2022 Mar 24;114:. doi: 10.3390/biology11040503.
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Digestive Enzyme Activities and Gut Emptying Are Correlated with the Reciprocal Regulation of TRPA1 Ion Channel and Serotonin in the Gut of the Sea Urchin Strongylocentrotus intermedius.
Ding J
,
Wang H
,
Li Z
,
Sun J
,
Ding P
,
Chi X
,
Yang M
,
Chang Y
,
Zhao C
.
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The energetic link in the benthic community is based on physiological characteristics of the low food absorption efficiency of sea urchins. Low food absorption efficiency of sea urchins is correlated with the activity of digestive enzymes and the duration of food in their gut. Thus, the digestive enzymes activities (pepsin and amylase enzyme activities) and gut emptying are important indicators in assessing nutrient digestion and absorption in sea urchins. In the present study, the relationship between these indicators and molecules related to digestive physiology were quantified in sea urchins. We found (1) an inter-regulatory relationship existed between Transient receptor potential cation channel, subfamily A, member 1 (TRPA1), and serotonin (5-hydroxytryptamine; 5-HT) in the gut of Strongylocentrotus intermedius; (2) digestive enzyme activities were negatively correlated with the TRPA1 and concentration of 5-HT in the gut of S. intermedius; (3) gut emptying rate was positively correlated with TRPA1 and concentration of 5-HT in the gut of S. intermedius. The present study revealed that the digestion and absorption of food are correlated with the TRPA1 and 5-HT in the gut of S. intermedius, which provides valuable information about the digestive physiology of sea urchins. This novel finding is relevant to understanding the low food digestibility of sea urchins. It also provides valuable information to the digestive physiology of sea urchins, which are key to maintaining the stability of food webs in the marine ecosystem.
41506177 National Natural Science Foundation of China, for Chong Zhao a grant for innovative talents in universities in Liaoning Province, for Yaqing Chang Chinese Outstanding Talents in Agricultural Sciences, FREU2020-02 Fund of Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, P. R. China
Figure 1. Diagram of the feces collection in each temperature-controlled tank.
Figure 2. (A) TRPA1 expression in the gut of Strongylocentrotus intermedius after the HC-030031 injection (n = 5). (B) The 5-HT level in the gut of S. intermedius after the 5-HT injection. The symbol *** means p < 0.001 (n = 5, mean ± s.e.m.).
Figure 3. TRPA1 is involved in food digestion of Strongylocentrotus intermedius. (A) The expression of TRPA1 in the gut of S. intermedius (n = 30). (B) TRPA1 expression of groups L and H (n = 5). (C,D) The activities of pepsin and amylase in the gut of the groups L and H (n = 5). The symbols ** and *** mean p < 0.01 and p < 0.001, respectively (mean ± s.e.m.).
Figure 4. An inter-regulatory relationship between TRPA1 and 5-HT in the gut of Strongylocentrotus intermedius. (A) The 5-HT level in the gut of groups L and H (n = 5). (B) TRPA1 expression after the 5-HT injection (n = 5). (C) The 5-HT level after the HC-030031 injection (n = 5). The symbol *** means p < 0.001, (mean ± s.e.m.).
Figure 5. Digestive enzyme activities are negatively correlated with the reciprocal regulation of TRPA1 and 5-HT in the gut of Strongylocentrotus intermedius. (A) The activities of pepsin and amylase of the control, DMSO, and HC-030031 groups (n = 5). (B) The activities of pepsin and amylase of the control, marine saline, and 5-HT groups (n = 5). (C) The activities of pepsin and amylase of control, DMSO + marine saline, and HC-030031 + 5-HT groups (n = 5). The symbol *** means p < 0.001 (mean ± s.e.m.).
Figure 6. Gut emptying is positively correlated with the reciprocal regulation of TRPA1 and 5-HT in the gut of Strongylocentrotus intermedius. (A) Dry weight of the 48-h accumulated feces of the control, DMSO, and HC-030031 groups (n = 5). (B) Dry weight of the 48-h accumulated feces of the control, marine saline, and 5-HT groups (n = 5). (C) The dry weight of the 48-h accumulated feces of the control, DMSO + marine saline, and HC-030031 + 5-HT groups (n = 5). The symbols ** and *** mean p < 0.01 and p < 0.001, respectively (mean ± s.e.m.).
Brothers,
Ocean warming alters predicted microbiome functionality in a common sea urchin.
2018, Pubmed,
Echinobase
Brothers,
Ocean warming alters predicted microbiome functionality in a common sea urchin.
2018,
Pubmed
,
Echinobase
Camilleri,
Effect of renzapride on transit in constipation-predominant irritable bowel syndrome.
2004,
Pubmed
Coleman,
Abnormalities of serotonin metabolism and their relation to symptoms in untreated celiac disease.
2006,
Pubmed
Damann,
TRPs in our senses.
2008,
Pubmed
Degen,
Effect of tegaserod on gut transit in male and female subjects.
2005,
Pubmed
Doihara,
TRPA1 agonists delay gastric emptying in rats through serotonergic pathways.
2009,
Pubmed
Eid,
HC-030031, a TRPA1 selective antagonist, attenuates inflammatory- and neuropathy-induced mechanical hypersensitivity.
2008,
Pubmed
Fothergill,
Effects of Food Components That Activate TRPA1 Receptors on Mucosal Ion Transport in the Mouse Intestine.
2016,
Pubmed
Francis,
Comparisons between the effects of 5-HT and DL-fenfluramine on food intake and gastric emptying in the rat.
1995,
Pubmed
Grundy,
5-HT system in the gut: roles in the regulation of visceral sensitivity and motor functions.
2008,
Pubmed
Han,
Molecular characterization and expression of SiFad1 in the sea urchin (Strongylocentrotus intermedius).
2019,
Pubmed
,
Echinobase
Holzer,
Transient receptor potential (TRP) channels as drug targets for diseases of the digestive system.
2011,
Pubmed
Horowitz,
Gastric emptying in diabetes: clinical significance and treatment.
2002,
Pubmed
Kim,
The TRPA1 agonist, methyl syringate suppresses food intake and gastric emptying.
2013,
Pubmed
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Macpherson,
Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines.
2007,
Pubmed
Mahé,
Gastrojejunal kinetics and the digestion of [15N]beta-lactoglobulin and casein in humans: the influence of the nature and quantity of the protein.
1996,
Pubmed
Nozawa,
TRPA1 regulates gastrointestinal motility through serotonin release from enterochromaffin cells.
2009,
Pubmed
Ohara,
β-Eudesmol, an oxygenized sesquiterpene, stimulates appetite via TRPA1 and the autonomic nervous system.
2017,
Pubmed
Pearse,
Ecological role of purple sea urchins.
2006,
Pubmed
,
Echinobase
Pedersen,
TRP channels: an overview.
2005,
Pubmed
Rehner,
Effect of proteins on availability of zinc. I. Gastrointestinal transit time of casein and whey protein and zinc absorption in weaned rats.
1985,
Pubmed
Shen,
Olfactory stimulation with scent of lavender oil affects autonomic nerves, lipolysis and appetite in rats.
NULL,
Pubmed
Yorke,
Sea urchins mediate the availability of kelp detritus to benthic consumers.
2019,
Pubmed
,
Echinobase
Yu,
TRP channel functions in the gastrointestinal tract.
2016,
Pubmed
Zhou,
[MYP gene expressions at transcription level in different stages of gonad of sea urchin Strongylocentrotus intermedius and hybrids].
2008,
Pubmed
,
Echinobase
