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Sci Rep
2022 May 17;121:8209. doi: 10.1038/s41598-022-12192-7.
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Cytotoxic and antimicrobial activities of two new sesquiterpenoids from red sea brittle star Ophiocoma dentata.
El Feky SE
,
Abd El Hafez MSM
,
Abd El Moneim NA
,
Ibrahim HAH
,
Okbah MA
,
Ata A
,
El Sedfy AS
,
Hussein A
.
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Bioactive compounds were extracted from a locally available brittle star; Ophiocoma dentata, collected from the Red Sea, Egypt. Two new sesquiterpenoids; 8, 11-epoxy-9(15)-himachaladiene-4-ol (O8-ophiocomane) and, 11-epoxy-9(15)-himachaladiene-4-ol (O7-ophiocomane) were isolated and characterized using appropriate techniques. Structure elucidation was estimated via 1D NMR, 2D NMR, FT-IR and mass spectroscopy analyses. The isolated compounds were tested for cytotoxic, antibacterial and antifungal activities. Pure compounds showed a dose dependent reduction in MCF-7 cells viability with LC50 of 103.5 and 59.5 μg/ml for compounds 1 and 2 respectively compared to the chemotherapeutic drug cisplatin (47.4 µg/ml). In vivo experiments showed that O. dentate extract significantly reduced tumor progression and improved hematological parameters and liver functions of tumor-bearing mice when administered either before or after tumor cells' injection. The most remarkable antimicrobial effects of O. dentate crude extract were against Staphylococcus aureus, Vibrio damsela and Pseudomonas aeruginosa while the pure compounds showed activity against P. aeruginosa alone. Neither the crude extract nor the pure compounds have shown activity against Aeromonas hydrophila. These results indicates that O. dentata extract and newly isolated compounds have shown a promising cytotoxic, antiproliferative and antimicrobial activities that might be useful for pharmaceutical applications.
Figure 1. Flow chart pure compounds’ extraction from O. dentate.
Figure 2. The 250 fractions combined (with the same RF value) fractions.
Figure 3. The (a) GC/MS and (b) FT-IR spectra of compound 1 (O8-Ophiocomane).
Figure 4. The (a) GC/MS and (b) FT-IR spectra of compound 2 (O7-Ophiocomane).
Figure 5. Cytotoxicity of O. dentata against MCF-7 cells: the graphs shows the cytotoxicity of (a) compound 1 and (b) compound 2 purified from O. dentata compared to (c) cisplatin.
Figure 6. In vivo biological effects of O. dentata in Ehrlich tumor-bearing mice including (a) tumor size measurements (b) hematological parameters and liver function tests in study groups.
Figure 7. Histopathological analysis of liver in vivo study using laboratory animal for cytotoxicity and anticancer activity, where picture (a) liver of a healthy control mouse, (b) liver of tumor-bearing mouse treated with O. dentata extract with no abnormal changes. (c) Ehrlich tumors of untreated mouse. (d) Ehrlich tumor of O. dentata treated mouse showing deeply chromatic pleomorphic nuclei with wide areas of necrosis.
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Abd El Hafez,
First report of steroid derivatives isolated from starfish Acanthaster planci with anti-bacterial, anti-cancer and anti-diabetic activities.
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,
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Afzali,
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2017,
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,
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Ahmed,
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2021,
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,
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Amini,
Metastatic Inhibitory and Radical Scavenging Efficacies of Saponins Extracted from the Brittle Star (Ophiocoma erinaceus).
2015,
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,
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Amini,
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2017,
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,
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Aung,
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2017,
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Baharara,
The anti-proliferative and anti-angiogenic effect of the methanol extract from brittle star.
2015,
Pubmed
,
Echinobase
Baharara,
The Potential of Brittle Star Extracted Polysaccharide in Promoting Apoptosis via Intrinsic Signaling Pathway.
2015,
Pubmed
,
Echinobase
Bauer,
Antibiotic susceptibility testing by a standardized single disk method.
1966,
Pubmed
Calixto-Campos,
The Ehrlich tumor induces pain-like behavior in mice: a novel model of cancer pain for pathophysiological studies and pharmacological screening.
2013,
Pubmed
Elshamy,
Paralemnolins X and Y, New Antimicrobial Sesquiterpenoids from the Soft Coral Paralemnalia thyrsoide.
2021,
Pubmed
Fang,
Two new sesquiterpenoids from the marine-sediment-derived fungus Trichoderma harzianum P1-4.
2019,
Pubmed
Gondim,
A taxonomic guide to the brittle-stars (Echinodermata, Ophiuroidea) from the State of Paraíba continental shelf, Northeastern Brazil.
2013,
Pubmed
,
Echinobase
Goto,
Highly efficient electro-gene therapy of solid tumor by using an expression plasmid for the herpes simplex virus thymidine kinase gene.
2000,
Pubmed
Guo,
Current progress on identification of virus pathogens and the antiviral effectors in echinoderms.
2021,
Pubmed
,
Echinobase
Huang,
Neolemnane-type sesquiterpenoids from a formosan soft coral Paralemnalia thyrsoides.
2007,
Pubmed
Kachooei,
Down-regulation of TGF-β, VEGF, and bFGF in vascular endothelial cells of chicken induced by a brittle star (Ophiocoma erinaceus) extract.
2020,
Pubmed
,
Echinobase
Klimenko,
A Cytotoxic Porphyrin from North Pacific Brittle Star Ophiura sarsii.
2020,
Pubmed
,
Echinobase
Lazzara,
Bright Spots in The Darkness of Cancer: A Review of Starfishes-Derived Compounds and Their Anti-Tumor Action.
2019,
Pubmed
,
Echinobase
Malve,
Exploring the ocean for new drug developments: Marine pharmacology.
2016,
Pubmed
Petzelt,
Are echinoderms of interest to biotechnology?
2005,
Pubmed
,
Echinobase
Pfeffer,
Apoptosis: A Target for Anticancer Therapy.
2018,
Pubmed
Pham,
Isolation of Sesquiterpenoids and Steroids from the Soft Coral Sinularia brassica and Determination of Their Absolute Configuration.
2021,
Pubmed
Ruiz-Torres,
An Updated Review on Marine Anticancer Compounds: The Use of Virtual Screening for the Discovery of Small-Molecule Cancer Drugs.
2017,
Pubmed
Stöhr,
Global diversity of brittle stars (Echinodermata: Ophiuroidea).
2012,
Pubmed
,
Echinobase
Sung,
Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries.
2021,
Pubmed
Watters,
Ascidian Toxins with Potential for Drug Development.
2018,
Pubmed
Zuo,
Development of Marine-Derived Compounds for Cancer Therapy.
2021,
Pubmed
van de Loosdrecht,
A tetrazolium-based colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia.
1994,
Pubmed