Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Evid Based Complement Alternat Med
2020 Nov 25;2020:1408926. doi: 10.1155/2020/1408926.
Show Gene links
Show Anatomy links
Effectiveness of Aqueous Extract of Marine Baitworm Marphysa moribidii Idris, Hutchings and Arshad, 2014 (Annelida, Polychaeta), on Acute Wound Healing Using Sprague Dawley Rats.
Rapi HS
,
Che Soh N'
,
Mohd Azam NS
,
Maulidiani M
,
Assaw S
,
Haron MN
,
Ali AM
,
Idris I
,
Ismail WIW
.
???displayArticle.abstract???
Wound healing is a well-coordinated process that restores skin integrity upon injury. However, some wound treatment poses harmful effects on the skin, which delay the normal wound healing process. Marphysa moribidii, a marine baitworm or polychaete, represents unique ability to regenerate posterior segment after injury, which may be beneficial in the wound healing treatment. The effectiveness of the polychaete as wound healing treatment was discovered through skin irritation, microbial testing, animal wound model, and chemical identifications. Three polychaete extracts (PE) emulsifying ointment (0.1%, 0.5%, and 1.0%) were topically applied to the full thickness wound model once daily for 14 days. Interestingly, PE 1.0% revealed the most rapid wound healing effects as compared to other treatments, including gamat (sea cucumber) oil (15% w/v) and acriflavine (0.1% w/v). Histopathological analysis using Masson's trichrome staining further confirms that PE treated wound exhibited minimal scar, high collagen deposition, and the emergence of neovascularisation. The extract also displayed a minimum inhibitory concentration (MIC) of 0.4 g/ml against Escherichia coli and absence of skin irritation, infectious bacteria, and heavy metals from the extract. Moreover, chemical compounds such as alkaloid, flavonoid, amino acids, and organic acid were detected in M. moribidii extracts, which could contribute to wound healing activity. In conclusion, this study further justifies the beneficial use of polychaete in treating wound healing and could be developed as a novel bioactive agent in nutraceuticals and pharmaceutical drugs.
Figure 1. Wound healing percentage (WHP) in different treatment groups was measured on days 3, 7, 11, and 14 after wounding. All treatments were applied once daily on rat's wound for 14 days (NO = no treatment (negative control), PCG = 15% positive control gamat, PCA = 0.1% positive control acriflavine, PE 0.1% = 0.1% polychaete extract, PE 0.5% = 0.5% polychaete extract, PE 1.0% = 1.0% polychaete extract prepared in cetomacrogol ointment, and NC = cetomacrogol ointment (negative control)). The result demonstrated that PE 1.0% showed rapid wound closure as compared with other treatments. Data for WHP from each group is tabulated in Supplementary Table S2. Data was analyzed using one-way ANOVA. ∗p < 0.05 indicates significant difference between PE 1.0% and negative control.
Figure 2. Hematoxylin and eosin stained histopathological section of granulation healing tissue of different groups at day 14. (a) NO = no treatment (negative control), (b) PCG = 15% gamat (positive control), (c) PCA = 0.1% acriflavine (positive control), (d) PE 0.1% = 0.1% polychaete extract, (e) PE 0.5% = 0.5% polychaete extract, (f) PE 1.0% = 1.0% polychaete extract prepared in cetomacrogol ointment, and (g) NC = cetomacrogol ointment (negative control). E = epidermis, S = scar/granulation tissue, C = collagen, F = fibroblast, H = hair follicle, and B = blood vessel. Smaller figure at upper left for each group was indicated by zoomed-out histological tissue at magnification 40x; Bar = 500 μm. Larger size figure in each group indicated the zoomed-in histological skin tissue and 200x; Bar = 100 μm.
Figure 3. Masson's trichome stained histopathological section of rat's skin tissue of different groups at day 14. (a) No treatment (negative control), (b) PCG = 15% positive control gamat, (c) PCA = 0.1% positive control acriflavine, (d) PE 0.1% = 0.1% polychaete extract, (e) PE 0.5% = 0.5% polychaete extract, (f) PE 1.0% = 1.0% polychaete extract prepared in cetomacrogol ointment, and (g) NC = cetomacrogol ointment (negative control). E = epidermis, S = scar/granulation tissue, C = collagen, F = fibroblast, H = hair follicle, B = blood vessel, CC = coarse collagen, and FC = fine collagen. The smaller figure at upper left for each group was indicated by zoomed-out histological tissue at magnification 40x; Bar = 500 μm. Larger size figure in each group indicated the zoomed-in histological skin tissue and 200x; Bar = 500 μm.
Auwal,
Preliminary phytochemical and elemental analysis of aqueous and fractionated pod extracts of Acacia nilotica (Thorn mimosa).
2014, Pubmed
Auwal,
Preliminary phytochemical and elemental analysis of aqueous and fractionated pod extracts of Acacia nilotica (Thorn mimosa).
2014,
Pubmed
Ayenimo,
Heavy metal exposure from personal care products.
2010,
Pubmed
Balouiri,
Methods for in vitro evaluating antimicrobial activity: A review.
2016,
Pubmed
Berthod,
Collagen fibril network and elastic system remodeling in a reconstructed skin transplanted on nude mice.
2001,
Pubmed
Bruno,
Worms' Antimicrobial Peptides.
2019,
Pubmed
Cano Sanchez,
Targeting Oxidative Stress and Mitochondrial Dysfunction in the Treatment of Impaired Wound Healing: A Systematic Review.
2018,
Pubmed
Coutinho,
A Review of "Polychaeta" Chemicals and their Possible Ecological Role.
2018,
Pubmed
Darestani,
Leech therapy for linear incisional skin-wound healing in rats.
2014,
Pubmed
Deng,
The effect of earthworm extract on promoting skin wound healing.
2018,
Pubmed
Grubbs,
Wound Physiology
2024,
Pubmed
Gul,
Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan.
2017,
Pubmed
Jarić,
Traditional wound-healing plants used in the Balkan region (Southeast Europe).
2018,
Pubmed
Junker,
Clinical Impact Upon Wound Healing and Inflammation in Moist, Wet, and Dry Environments.
2013,
Pubmed
Li,
Validating Rat Model of Empathy for Pain: Effects of Pain Expressions in Social Partners.
2018,
Pubmed
Marcinkiewicz,
Taurine and inflammatory diseases.
2014,
Pubmed
Medrado,
Phenotype characterization of pericytes during tissue repair following low-level laser therapy.
2010,
Pubmed
Opp,
Cytokines and sleep.
2005,
Pubmed
Rebelo,
Arsenic, lead, mercury and cadmium: Toxicity, levels in breast milk and the risks for breastfed infants.
2016,
Pubmed
Robson,
Wound infection. A failure of wound healing caused by an imbalance of bacteria.
1997,
Pubmed
Sen,
Human Wounds and Its Burden: An Updated Compendium of Estimates.
2019,
Pubmed
Sá,
Effects of Glycine on Collagen, PDGF, and EGF Expression in Model of Oral Mucositis.
2018,
Pubmed
Thakur,
Practices in wound healing studies of plants.
2011,
Pubmed
Udhayakumar,
Novel fibrous collagen-based cream accelerates fibroblast growth for wound healing applications: in vitro and in vivo evaluation.
2017,
Pubmed
Velnar,
The wound healing process: an overview of the cellular and molecular mechanisms.
2009,
Pubmed
Vukojević,
Hyperalgesia-type response reveals no difference in pain-related behavior between Wistar and Sprague-Dawley rats.
2007,
Pubmed
Wong,
Prevalence and antibiotic susceptibility of bacteria from acute and chronic wounds in Malaysian subjects.
2015,
Pubmed