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Biomed Res Int
2019 Jan 01;2019:1614024. doi: 10.1155/2019/1614024.
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Application of a Promising Bone Graft Substitute in Bone Tissue Regeneration: Characterization, Biocompatibility, and In Vivo Animal Study.
Wahyuningtyas E
,
Hsu LC
,
Lan WC
,
Wen SC
,
Ou KL
,
Chou HH
,
Huang MS
,
Sugiatno E
.
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The purpose of the present study was to investigate the effect of local hydroxyapatite (HA) combined with extracted sea cucumber (Stichopus hermanni) collagen as a promising bone graft substitute on bone remodeling. Fourier-transform infrared spectroscopy, X-ray diffractometry, transmission electron microscopy, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and Sprague-Dawley rat model were used to characterize the microstructure, in vitro cytotoxicity, and in vivo bone-healing properties of the investigated biocomposite material. Analytical results found that the hydrothermal reaction-synthesized local HA had a hexagonal close-packed structure. The addition of extracted S. hermanni collagen did not influence the microstructure and functional groups of the local HA. Moreover, the MTT assay indicated that the investigated biocomposite material possessed a good in vitro biocompatibility. The in vivo animal study also revealed that the investigated biocomposite material exhibited the highest number of osteoblasts after 14 days of healing. Therefore, the results demonstrate that the local HA combined with extracted S. hermanni collagen could potentially enhance osteoblast formation in promoting bone healing and regeneration.
Figure 1. FTIR spectra of the local HA with and without S. hermanni collagen samples.
Figure 2. XRD patterns of the local HA with and without S. hermanni collagen samples.
Figure 3. TEM micrographs of (a) the local HA sample and (b) the local HA with the S. hermanni collagen sample (the ring spots are indicated by white arrows).
Figure 4. Histological images of the investigated samples after 14 days of healing. (a) Group I: local HA with S. hermanni collagen. (b) Group II: only S. hermanni collagen. (c) Group III: only normal collagen (the osteoblasts are indicated by yellow and black arrows).
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