Paranaiba LF et al. (2019), Extracellular vesicles isolated from Trypanosom...

ECB-ART-48739

Mem Inst Oswaldo Cruz 2019 Jan 01;114:e190217. doi: 10.1590/0074-02760190217.

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Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans.

Paranaiba LF , Guarneri AA , Torrecilhas AC , Melo MN , Soares RP .

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The protozoan Trypanosoma cruzi has the ability to spontaneously secrete extracellular vesicles (EVs). In this paper, T. cruzi EVs derived from epimastigote forms were evaluated during interaction with triatomine bugs Rhodnius prolixus and Triatoma infestans. T. cruzi EVs were purified and artificially offered to the insects prior to infection with epimastigote forms. No effect of EVs was detected in the parasite counts in the guts of both vectors after 49-50 days. On the other hand, pre-feeding with EVs delayed parasite migration to rectum only in the gut in R. prolixus after 21-22 days. Those data suggest a possible role of T. cruzi EVs during the earlier events of infection in the invertebrate host.

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Genes referenced: srpl

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References [+] :

Alves, Trypanosoma cruzi: adhesion to the host cell and intracellular survival. 2007, Pubmed

	Fig. 2:. extracellular vesicles (EVs) of Trypanosoma cruzi. (A) Scanning electron microscopy (SEM) of T. cruzi membrane shedding (bars: 1-5 µm), Magnification 32,657 x and (B) Scanning electron microscopy (SEM) of T. cruzi membrane shedding (bars: 1-5 µm) Magnification 80,000 x. Nanoparticle tracking analysis (NTA) (C) and (D) of T. cruzi EVs.
	Fig. 3:. extracellular vesicles (EVs) of Trypanosoma cruzi. Transmission electron microscopy (TEM) of T. cruzi membrane shedding (A) (lower magnification, bar: 200 nm) and (B) (higher magnification, bar: 100 nm).
	Fig. 4:. parasites found throughout the digestive tract of Rhodnius prolixus. Dissections at 21-22 (A) and 49-50 (B) days after the infective feeding. Each dot represents the quantification of parasites from one individual nymph and each horizontal bar corresponds to the median of the group evaluated.
	Fig. 5:. parasites found throughout the digestive tract of Triatoma infestans. Dissections at 21-22 (A) e 49-50 (B) days after the infective feeding. Each dot represents the quantification of parasites from one individual nymph and each horizontal bar corresponds to the median of the group evaluated.
	Fig. 6:. metacyclic trypomastigotes found in the urine of Rhodnius prolixus (A) and Triatoma infestans (B) at 28 days p.i. Each dot represents the quantification of parasites in the urine sample of one nymph and each horizontal bar corresponds to the mean of the group evaluated.