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J Biol Chem
2020 Mar 06;29510:3159-3172. doi: 10.1074/jbc.RA119.011701.
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Sulfated and sialylated N-glycans in the echinoderm Holothuria atra reflect its marine habitat and phylogeny.
Vanbeselaere J
,
Jin C
,
Eckmair B
,
Wilson IBH
,
Paschinger K
.
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Among the earliest deuterostomes, the echinoderms are an evolutionary important group of ancient marine animals. Within this phylum, the holothuroids (sea cucumbers) are known to produce a wide range of glycoconjugate biopolymers with apparent benefits to health; therefore, they are of economic and culinary interest throughout the world. Other than their highly modified glycosaminoglycans (e.g. fucosylated chondroitin sulfate and fucoidan), nothing is known about their protein-linked glycosylation. Here we used multistep N-glycan fractionation to efficiently separate anionic and neutral N-glycans before analyzing the N-glycans of the black sea cucumber (Holothuria atra) by MS in combination with enzymatic and chemical treatments. These analyses showed the presence of various fucosylated, phosphorylated, sialylated, and multiply sulfated moieties as modifications of oligomannosidic, hybrid, and complex-type N-glycans. The high degree of sulfation and fucosylation parallels the modifications observed previously on holothuroid glycosaminoglycans. Compatible with its phylogenetic position, H. atra not only expresses vertebrate motifs such as sulfo- and sialyl-Lewis A epitopes but displays a high degree of anionic substitution of its glycans, as observed in other marine invertebrates. Thus, as for other echinoderms, the phylum- and order-specific aspects of this species'' N-glycosylation reveal both invertebrate- and vertebrate-like features.
Aoki,
Dynamic developmental elaboration of N-linked glycan complexity in the Drosophila melanogaster embryo.
2007, Pubmed
Aoki,
Dynamic developmental elaboration of N-linked glycan complexity in the Drosophila melanogaster embryo.
2007,
Pubmed
Cabrera,
Structural characterization and biological implications of sulfated N-glycans in a serine protease from the neotropical moth Hylesia metabus (Cramer [1775]) (Lepidoptera: Saturniidae).
2016,
Pubmed
Correia-da-Silva,
Anticancer and cancer preventive compounds from edible marine organisms.
2017,
Pubmed
,
Echinobase
Dolmatov,
Asexual reproduction in holothurians.
2014,
Pubmed
,
Echinobase
Dragosits,
Recombinant Aspergillus β-galactosidases as a robust glycomic and biotechnological tool.
2014,
Pubmed
Eckmair,
Glycosylation at an evolutionary nexus: the brittle star Ophiactis savignyi expresses both vertebrate and invertebrate N-glycomic features.
2020,
Pubmed
,
Echinobase
Eckmair,
Multistep Fractionation and Mass Spectrometry Reveal Zwitterionic and Anionic Modifications of the N- and O-glycans of a Marine Snail.
2016,
Pubmed
Engle,
The glycan CA19-9 promotes pancreatitis and pancreatic cancer in mice.
2019,
Pubmed
Green,
The asparagine-linked oligosaccharides on bovine fetuin. Structural analysis of N-glycanase-released oligosaccharides by 500-megahertz 1H NMR spectroscopy.
1988,
Pubmed
Guérardel,
Sialome analysis of the cephalochordate Branchiostoma belcheri, a key organism for vertebrate evolution.
2012,
Pubmed
,
Echinobase
Harduin-Lepers,
The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach.
2005,
Pubmed
,
Echinobase
Harvey,
Fragmentation of negative ions from N-linked carbohydrates, part 4. Fragmentation of complex glycans lacking substitution on the 6-antenna.
2010,
Pubmed
Harvey,
Composition of N-linked carbohydrates from ovalbumin and co-purified glycoproteins.
2000,
Pubmed
Hase,
Reexamination of the pyridylamination used for fluorescence labeling of oligosaccharides and its application to glycoproteins.
1984,
Pubmed
Honke,
Sulfotransferases and sulfated oligosaccharides.
2002,
Pubmed
Hykollari,
Analysis of Invertebrate and Protist N-Glycans.
2017,
Pubmed
Hykollari,
Mass spectrometric analysis of neutral and anionic N-glycans from a Dictyostelium discoideum model for human congenital disorder of glycosylation CDG IL.
2013,
Pubmed
Hykollari,
More Than Just Oligomannose: An N-glycomic Comparison of Penicillium Species.
2016,
Pubmed
Hykollari,
Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.
2019,
Pubmed
Ishimizu,
Presence of asparagine-linked N-acetylglucosamine and chitobiose in Pyrus pyrifolia S-RNases associated with gametophytic self-incompatibility.
1999,
Pubmed
Janakiram,
Sea Cucumbers Metabolites as Potent Anti-Cancer Agents.
2015,
Pubmed
,
Echinobase
Jiménez-Castells,
Development of a multifunctional aminoxy-based fluorescent linker for glycan immobilization and analysis.
2016,
Pubmed
Karamanos,
Isolation, biochemical and immunological characterisation of two sea urchin glycoproteins bearing sulphated poly(sialic acid) polysaccharides rich in N-glycolyl neuraminic acid.
1996,
Pubmed
,
Echinobase
Kisa,
Constituents of Holothuroidea, 18. Isolation and structure of biologically active disialo- and trisialo-gangliosides from the sea cucumber Cucumaria echinata.
2006,
Pubmed
,
Echinobase
Koles,
Functional characterization of Drosophila sialyltransferase.
2004,
Pubmed
Kollmann,
Mannose phosphorylation in health and disease.
2010,
Pubmed
Kurz,
Hemocytes and plasma of the eastern oyster (Crassostrea virginica) display a diverse repertoire of sulfated and blood group A-modified N-glycans.
2013,
Pubmed
Lamriben,
N-Glycan-based ER Molecular Chaperone and Protein Quality Control System: The Calnexin Binding Cycle.
2016,
Pubmed
Mertsalov,
Characterization of Drosophila CMP-sialic acid synthetase activity reveals unusual enzymatic properties.
2016,
Pubmed
Miyagawa,
A comparison of the main structures of N-glycans of porcine islets with those from humans.
2014,
Pubmed
Mou,
Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.
2017,
Pubmed
,
Echinobase
Muchmore,
Biosynthesis of N-glycolyneuraminic acid. The primary site of hydroxylation of N-acetylneuraminic acid is the cytosolic sugar nucleotide pool.
1989,
Pubmed
Murakami,
Structure determination of a sulfated N-glycans, candidate for a precursor of the selectin ligand in bovine lung.
2007,
Pubmed
Oku,
Studies on the substrate specificity of neutral alpha-mannosidase purified from Japanese quail oviduct by using sugar chains from glycoproteins.
1991,
Pubmed
Paschinger,
Anionic and zwitterionic moieties as widespread glycan modifications in non-vertebrates.
2020,
Pubmed
Paschinger,
Comparisons of N-glycans across invertebrate phyla.
2019,
Pubmed
Paschinger,
Analysis of zwitterionic and anionic N-linked glycans from invertebrates and protists by mass spectrometry.
2016,
Pubmed
Schlenzka,
Catalytic properties of the CMP-N-acetylneuraminic acid hydroxylase from the starfish Asterias rubens: comparison with the mammalian enzyme.
1993,
Pubmed
,
Echinobase
Silchenko,
Colochiroside E, an Unusual Non-holostane Triterpene Sulfated Trioside from the Sea Cucumber Colochirus robustus and Evidence of the Impossibility of a 7(8)-Double Bond Migration in Lanostane Derivatives having an 18(16)-Lactone.
2016,
Pubmed
,
Echinobase
Stanton,
The underestimated N-glycomes of lepidopteran species.
2017,
Pubmed
Taguchi,
Occurrence and structural analysis of highly sulfated multiantennary N-linked glycan chains derived from a fertilization-associated carbohydrate-rich glycoprotein in unfertilized eggs of Tribolodon hakonensis.
1996,
Pubmed
Thompson,
Structural and mechanistic insight into N-glycan processing by endo-α-mannosidase.
2012,
Pubmed
Varki,
Symbol Nomenclature for Graphical Representations of Glycans.
2015,
Pubmed
Vaughn,
Sequencing and analysis of the gastrula transcriptome of the brittle star Ophiocoma wendtii.
2012,
Pubmed
,
Echinobase
Wilson,
Analysis of Asn-linked glycans from vegetable foodstuffs: widespread occurrence of Lewis a, core alpha1,3-linked fucose and xylose substitutions.
2001,
Pubmed
Yagi,
Development of structural analysis of sulfated N-glycans by multidimensional high performance liquid chromatography mapping methods.
2005,
Pubmed
Yamada,
Constituents of holothuroidea, 12. Isolation and structure of glucocerebrosides from the sea cucumber Holothuria pervicax.
2002,
Pubmed
,
Echinobase
Yamada,
Constituents of holothuroidea. 10. Isolation and structure of a biologically active ganglioside molecular species from the sea cucumber Holothuria leucospilota.
2001,
Pubmed
,
Echinobase
Yamakawa,
Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns.
2018,
Pubmed
Yamashita,
Oligosaccharides of human milk. Structural studies of two new octasaccharides, difucosyl derivatives of para-lacto-N-hexaose and para-lacto-N-neohexaose.
1977,
Pubmed
Yan,
Comparison of RP-HPLC modes to analyse the N-glycome of the free-living nematode Pristionchus pacificus.
2015,
Pubmed
Yu,
Enabling techniques and strategic workflow for sulfoglycomics based on mass spectrometry mapping and sequencing of permethylated sulfated glycans.
2009,
Pubmed
