ECB-ART-47888
Biochem Biophys Res Commun
2003 Jan 03;3001:121-7. doi: 10.1016/s0006-291x(02)02797-3.
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Cybip, a starfish cyclin B-binding protein, is involved in meiotic M-phase exit.
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We designed a screen to identify starfish oocyte proteins able to bind monomeric cyclin B by affinity chromatography on a cyclin B splice variant displaying low affinity for cdc2. We identified a 15kDa protein previously described as a cdk-binding protein [Biochim. Biophys. Acta Mol. Cell Res. 1589 (2002) 219-231]. Cybip is encoded by a single polymorphic gene and the native protein is matured by cleaving a signal peptide. We firmly establish the fact that it is a true cyclin B-binding protein, since the recombinant protein binds recombinant cyclin B in absence of any cdk. Finally, we show that the microinjection of GST-cybip, and of anti-cybip antibody, in maturing starfish oocytes, inhibits H1 kinase and MPF inactivation, and first polar body emission.
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Genes referenced: LOC115919910 LOC577317 LOC594349