Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Am Soc Mass Spectrom
2002 Jul 01;137:784-91. doi: 10.1016/S1044-0305(02)00393-8.
Show Gene links
Show Anatomy links
De novo sequencing of peptides using MALDI/TOF-TOF.
Yergey AL
,
Coorssen JR
,
Backlund PS
,
Blank PS
,
Humphrey GA
,
Zimmerberg J
,
Campbell JM
,
Vestal ML
.
???displayArticle.abstract???
The recently developed MALDI TOF-TOF instrument yields relatively complex but interpretable fragmentation spectra. When coupled with a straightforward sequence extension algorithm, it is possible to develop complete peptide sequences de novo from the spectra. This approach has been applied to a set of peptides derived from typtic digestion of electrophoretically separated sea urchin egg membrane proteins. When directed to proteins that have been described previously, the results were in essential agreement with those obtained by conventional data base searching approaches, with certain important exceptions. The present method detected errors in published sequences and was able to develop sequences from peptides differing in mass by one dalton (Da). These results show both the power of the present approach and the need for using de novo methods more frequently than may be otherwise appreciated.
Biemann,
Determination of the amino acid sequence in oligopeptides by computer interpretation of their high-resolution mass spectra.
1966, Pubmed
Biemann,
Determination of the amino acid sequence in oligopeptides by computer interpretation of their high-resolution mass spectra.
1966,
Pubmed
Chen,
A dynamic programming approach to de novo peptide sequencing via tandem mass spectrometry.
2001,
Pubmed
Clauser,
Rapid mass spectrometric peptide sequencing and mass matching for characterization of human melanoma proteins isolated by two-dimensional PAGE.
1995,
Pubmed
Clauser,
Role of accurate mass measurement (+/- 10 ppm) in protein identification strategies employing MS or MS/MS and database searching.
1999,
Pubmed
Coorssen,
Biochemical and functional studies of cortical vesicle fusion: the SNARE complex and Ca2+ sensitivity.
1998,
Pubmed
,
Echinobase
Dancík,
De novo peptide sequencing via tandem mass spectrometry.
1999,
Pubmed
Davis,
A microscale electrospray interface for on-line, capillary liquid chromatography/tandem mass spectrometry of complex peptide mixtures.
1995,
Pubmed
Egelhofer,
Improvements in protein identification by MALDI-TOF-MS peptide mapping.
2000,
Pubmed
Eng,
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.
1994,
Pubmed
Eriksson,
A statistical basis for testing the significance of mass spectrometric protein identification results.
2000,
Pubmed
Hines,
Pattern-based algorithm for peptide sequencing from tandem high energy collision-induced dissociation mass spectra.
1992,
Pubmed
Johnson,
Computer program (SEQPEP) to aid in the interpretation of high-energy collision tandem mass spectra of peptides.
1989,
Pubmed
Mann,
Error-tolerant identification of peptides in sequence databases by peptide sequence tags.
1994,
Pubmed
Mann,
Use of mass spectrometric molecular weight information to identify proteins in sequence databases.
1993,
Pubmed
Perkins,
Probability-based protein identification by searching sequence databases using mass spectrometry data.
1999,
Pubmed
Siegel,
An efficient algorithm for sequencing peptides using fast atom bombardment mass spectral data.
1988,
Pubmed
Tahara,
Calcium can disrupt the SNARE protein complex on sea urchin egg secretory vesicles without irreversibly blocking fusion.
1998,
Pubmed
,
Echinobase
Taylor,
Sequence database searches via de novo peptide sequencing by tandem mass spectrometry.
1997,
Pubmed
Taylor,
Implementation and uses of automated de novo peptide sequencing by tandem mass spectrometry.
2001,
Pubmed
Tsugita,
Exopeptidase digestion in combination with field desorption mass spectrometry for amino acid sequence determination.
1982,
Pubmed
Zhang,
ProFound: an expert system for protein identification using mass spectrometric peptide mapping information.
2000,
Pubmed
Zhang,
De novo peptide sequencing by two-dimensional fragment correlation mass spectrometry.
2000,
Pubmed
Zimmerberg,
A stage-specific preparation to study the Ca(2+)-triggered fusion steps of exocytosis: rationale and perspectives.
2000,
Pubmed
,
Echinobase
