ECB-ART-47688
J Chromatogr B Analyt Technol Biomed Life Sci
2002 Apr 25;7701-2:71-5. doi: 10.1016/s1570-0232(01)00628-6.
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Separation of phospholipid classes in sea urchin, paracentrotus lividus by high-performance liquid chromatography.
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A simple high-performance liquid chromatography (HPLC) method for determination of major phospholipid classes in sea urchin Paracentrotus lividus is described. The separation was performed on a Tracer Extrasil SI 5 microm 25 x 0.4 cm column and an isocratic mobile phase of acetonitrile-methanol 85%-phosphoric acid (50:50:1.8, v/v). The HPLC method utilizes UV detection at 205 nm. Five phospholipids were identified and quantified: phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC) and sphingomyelin (SM). Fresh and canned samples were analyzed. Student''s t-test showed no significant difference (P < or = 0.05) between the mean phospholipid contents of raw and canned sea urchin.
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Genes referenced: LOC100887844