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In Vitro and in Vivo Scolicidal Activities of Holothuria leucospilota Extract and CeO2 Nanoparticles against Hydatid Cyst.
Aryamand S
,
Khademvatan S
,
Hazrati Tappeh K
,
Heshmatian B
,
Jelodar A
.
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Background: We aimed to investigate the scolicidal effects of Holothuria leucospilota extract and CeO2 nanoparticles against protoscoleces of hydatid cysts in-vitro and in-vivo.
Methods: Hydatid cysts were collected from, Urmia slaughterhouses between years 2016-2017 and the hydatid fluid aspirated from the fertile cysts. Various concentration of H. leucospilota extract, CeO2 NPs and combination of CeO2-NPs/H. leucospilota were used for 10-60 min to evaluate the viability of protoscoleces by 0.1% eosin method. CASPASE -3 activity measured for assessment of cell apoptosis in treated protoscoleces. BALB/c mice were infected intraperitoneally with 2000 viable protoscoleces and treated daily for 4 wk by intragastrical inoculation with H. leucospilota, CeO2 NPs, combination of CeO2 NPs/H. leucospilota and Albendazole. Cyst development was macroscopically analyzed.
Results: H. leucospilota extract and combination of CeO2 NPs/H. leucospilota have potent scolicidal activity at concentration of 20 mg/ml and 15 mg/ml after 60 min treatment. Maximum caspase-3 activity was observed when protoscoleces expose with H. leucospilota and combination H. leucospilota & CeO2 NPs. After treatment of cyst infected mice with extract and CeO2 NPs, combination of CeO2 NPs/H leucospilota and albendazole, a significant decrease in number of cysts, size and volume of cyst (P<0.05) was observed.
Conclusion: This result shows an antihydatic and scolicidal effects of H. leucospilota extract and CeO2 NPs.
Fig. 1:. Dead protoscoleces of E. granulosus after exposure with 0.1% eosin
Fig. 2:. Live protoscoleces of E. granulosus after exposure with 0.1% eosin
Fig. 4:. CASPASE -3 activity assay in the three induced groups (CeO2 NPs: 10mg/ml, H. leucospilota extract: 20 mg/ml, combination CeO2 and H. leucospilota extract: 15 mg/ml) and control groups (albendazole as positive control group and PBS as negative control group)
Fig. 5:. After two months, mice infected with cysts in peritoneal cavity and liver (by injection of 2000 viable protoscoleces in /0.5mL sterile PBS)
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