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Mar Drugs
2019 Jul 05;177:. doi: 10.3390/md17070397.
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Quantitative Proteome Reveals Variation in the Condition Factor of Sea Urchin Strongylocentrotus nudus during the Fishing Season Using an iTRAQ-based Approach.
Shang WH
,
Han JR
,
Yan JN
,
Du YN
,
Xu YS
,
Xue CF
,
Zhang TT
,
Wu HT
,
Zhu BW
.
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To investigate the variation in the condition factor of the sea urchin Strongylocentrotus nudus (S. nudus), gonads were collected in May (MAY), June (JUN), and July (JUL), at the beginning (AUG-b) and end of August (AUG-e). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) detection of the gonads revealed an obvious enhancement of the band at about 37 kDa from July, which was identified as transforming growth factor-beta-induced protein ig-h3 (TGFBI) by nanoLC-ESI-MS/MS. Gonadal proteins were identified by isobaric tagging for relative and absolute quantitation (iTRAQ), and regulation of the identified proteins in pairs of the collected groups was observed. A total of 174 differentially expressed proteins (DEPs) were identified. Seven of the DEPs showed significant correlations with both the gonad index (GI) and protein content. These correlations included 6-phosphogluconate dehydrogenase, decarboxylating isoform X2 (6PGD), CAD protein, myoferlin isoform X8, ribosomal protein L36 (RL36), isocitrate dehydrogenase [NADP], mitochondrialisoform X2 (IDH), multifunctional protein ADE2 isoform X3, sperm-activating peptides (SAPs) and aldehyde dehydrogenase, and mitochondrial (ALDH). However, TGFBI had no correlation with gonad index (GI) or protein content. 6PGD, IDH, multifunctional protein ADE2isoform X3, and ALDH were shown to interact with each other and might play key roles in changing the condition factor of S. nudus gonads.
Figure 1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of gonad proteins in S. nudus. Lane 1, protein marker. The gonads collected in May (MAY), June (JUN), July (JUL), at the beginning of August. and at the end of August are shown in lanes 2–4, 5–7, 8–10, 11–13 and 14–16, respectively. Aug-b, the beginning of August; Aug-e, the end of August.
Figure 2. The number of upregulated and downregulated differentially expressed proteins (DEPs) identified in the compared groups (JUN–MAY, JUL–JUN, AUG-b-JUL, and AUG-e-AUG-b).
Figure 3. Gene ontology (GO) annotation of the upregulated and downregulated differentially expressed proteins (DEPs) identified in compared groups (JUN–MAY, JUL–JUN, AUG-b-JUL, and AUG-e-AUG-b) including biological processes, molecular functions, and cellular components.
Figure 4. The protein-protein interaction (PPI) analysis among the differentially expressed proteins (DEPs) related to both gonad index (GI) and protein content in the compared groups (JUN–MAY, JUL–JUN, AUG-b-JUL, and AUG-e-AUG-b). Red nodes represent upregulated proteins; blue nodes represent downregulated proteins; black line: co-expression.
Figure 5. Experimental design and workflow for the quantitative proteomic analysis of S. nudus gonads using the isobaric tagging for relative and absolute quantitation (iTRAQ) labelling proteomic strategy.
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