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Mem Inst Oswaldo Cruz
2019 Jan 01;114:e190020. doi: 10.1590/0074-02760190020.
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Diversity of metallo-β-lactamase-encoding genes found in distinct species of Acinetobacter isolated from the Brazilian Amazon Region.
Brasiliense D
,
Cayô R
,
Streling AP
,
Nodari CS
,
Barata RR
,
Lemos PS
,
Massafra JM
,
Correa Y
,
Magalhães I
,
Gales AC
,
Sodré R
.
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BACKGROUND: The multidrug resistance (MDR) phenotype is frequently observed in Acinetobacter baumannii, the most clinically relevant pathogenic species of its genus; recently, other species belonging to the A. calcoaceticus-A. baumannii complex have emerged as important MDR nosocomial pathogens.
OBJECTIVES: The present study aimed to verify the occurrence of metallo-β-lactamase genes among distinct Acinetobacter species in a hospital located in the Brazilian Amazon Region.
METHODS: Antimicrobial susceptibility profiles were determined by broth microdilution. The genetic relationships among these isolates were assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Pyrosequencing reads of plasmids carrying the bla NDM-1 gene were generated using the Ion Torrent™ platform sequencing.
FINDINGS: A total of six isolates carried bla NDM-1: A. baumannii (n = 2), A. nosocomialis (n = 3), and A. pittii (n = 1); three carried bla IMP-1: A. baumannii, A. nosocomialis, and A. bereziniae. Resistance to colistin was observed for an NDM-1-producing A. nosocomialis isolate. Diverse PFGE patterns and sequence types were found among A. nosocomialis and A. baumannii isolates. The bla NDM-1 sequence was inserted in a Tn125 transposon, while the bla IMP-1 was found as a gene cassette of the class 1 integron In86.
MAIN CONCLUSIONS: To the best of our knowledge, this is the first report describing the dissemination of bla NDM-1 among distinct Acinetobacter species recovered from the same hospital in South America.
Fig. 2:. (A) circular map of plasmid pIEC383 recovered from
Acinetobacter baumannii IEC383. The yellow arrows
represent the coding sequences and the direction of transcription. HP:
hypothetical protein. The Tn125 region can be observed.
(B) Circular map of plasmid pIEC38057 recovered from A.
nosocomialis IEC38057. The legend of Fig. 2B was same as
that of Fig. 2A. (C) Partial sequence of plasmid pIEC37710 recovered
from A. pittii IEC37710.
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