Zhan Y et al. (2019), The Impact of Chronic Heat Stress on the Growth...

ECB-ART-47156

Front Genet 2019 Feb 19;10:301. doi: 10.3389/fgene.2019.00301.

Show Gene links Show Anatomy links

The Impact of Chronic Heat Stress on the Growth, Survival, Feeding, and Differential Gene Expression in the Sea Urchin Strongylocentrotus intermedius.

Zhan Y , Li J , Sun J , Zhang W , Li Y , Cui D , Hu W , Chang Y .

???displayArticle.abstract???
To explore the impact of chronic heat stress on commercial echinoderms, the present study assessed the effects of chronic high temperature on the growth, survival, feeding, and differential gene expression in the sea urchin Strongylocentrotus intermedius cultured in northern Yellow Sea in China. One suitable seawater condition (20°C) and one laboratory-controlled high temperature condition (25°C) were set up. After 28 days incubation, our results showed that: (1) The specific growth, survival, and ingestion rates of S. intermedius reared under high temperature (25°C) decreased compared to those reared under optimal temperature (20°C) conditions; (2) comparative transcriptome analysis identified 2,125 differentially expressed genes (DEGs) in S. intermedius reared under high temperature (25°C) compared to those subjected to optimal temperature condition (20°C), which included 1,015 upregulated and 1,100 downregulated genes. The accuracy of the transcriptome profiles was verified by quantitative real-time PCR (qRT-PCR). Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyses revealed that these DEGs mainly enriched the functional categories of ribosome, protein processing in endoplasmic reticulum, and prion diseases. A total of 732 temperature-induced expressed genes, such as ATP5, heat shock protein 70, and heat shock protein 90, were identified as candidates that were closely correlated with heat resistance in S. intermedius. Differentially expressed transcription factors (TFs), such as AP-1, Fos, CREB, and ZNF, were also identified as potential regulators that regulate the molecular network that was associated with responses to heat stress in sea urchins. Observations in the present study provide additional information that improves our understanding of the molecular mechanism of temperate echinoid species in response to heat stress, as well as theoretical basis for the molecular-assisted breeding of heat-resistant sea urchins.

???displayArticle.pubmedLink??? 31019527
???displayArticle.pmcLink??? PMC6458246
???displayArticle.link??? Front Genet

Genes referenced: creb1 impact iqgap1 LOC100887844 LOC100892721 LOC105439191 LOC579838 LOC583082

???attribute.lit??? ???displayArticles.show???

References [+] :

Branco, The impact of rising sea temperature on innate immune parameters in the tropical subtidal sea urchin Lytechinus variegatus and the intertidal sea urchin Echinometra lucunter. 2013, Pubmed , Echinobase
Byrne, Temperature, but not pH, compromises sea urchin fertilization and early development under near-future climate change scenarios. 2009, Pubmed , Echinobase
Cen, An analog of a dipeptide-like structure of FK506 increases glial cell line-derived neurotrophic factor expression through cAMP response element-binding protein activated by heat shock protein 90/Akt signaling pathway. 2006, Pubmed
Chang, Family Growth and Survival Response to Two Simulated Water Temperature Environments in the Sea Urchin Strongylocentrotus intermedius. 2016, Pubmed , Echinobase
Cunha, Sea-urchin (Paracentrotus lividus) glutathione S-transferases and cholinesterase activities as biomarkers of environmental contamination. 2005, Pubmed , Echinobase
Doong, CAIR-1/BAG-3 abrogates heat shock protein-70 chaperone complex-mediated protein degradation: accumulation of poly-ubiquitinated Hsp90 client proteins. 2003, Pubmed
Fang, De novo RNA sequencing transcriptome of Rhododendron obtusum identified the early heat response genes involved in the transcriptional regulation of photosynthesis. 2017, Pubmed
Grabherr, Full-length transcriptome assembly from RNA-Seq data without a reference genome. 2011, Pubmed
Hirayama, Common pathways in circadian and cell cycle clocks: light-dependent activation of Fos/AP-1 in zebrafish controls CRY-1a and WEE-1. 2005, Pubmed
Iijima-Ando, cAMP-response element-binding protein and heat-shock protein 70 additively suppress polyglutamine-mediated toxicity in Drosophila. 2005, Pubmed
Kiang, Heat shock protein 70 kDa: molecular biology, biochemistry, and physiology. 1998, Pubmed
Koo, Mind bomb-2 is an E3 ligase for Notch ligand. 2005, Pubmed
Li, RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. 2011, Pubmed
Livak, Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. 2001, Pubmed
McKenna, The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. 2010, Pubmed
Moreira, The use of Mytilus galloprovincialis acetylcholinesterase and glutathione S-transferases activities as biomarkers of environmental contamination along the northwest Portuguese coast. 2005, Pubmed
Oommen, Co-regulation by Notch and Fos is required for cell fate specification of intermediate precursors during C. elegans uterine development. 2007, Pubmed
Padilla-Gamiño, Temperature and CO(2) additively regulate physiology, morphology and genomic responses of larval sea urchins, Strongylocentrotus purpuratus. 2013, Pubmed , Echinobase
Pörtner, Ecology. Physiology and climate change. 2008, Pubmed
Pratt, The hsp90-based chaperone system: involvement in signal transduction from a variety of hormone and growth factor receptors. 1998, Pubmed
Qu, A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis. 2015, Pubmed
Robison, Antisense expression of mitochondrial ATP synthase subunits OSCP (ATP5) and gamma (ATP3) alters leaf morphology, metabolism and gene expression in Arabidopsis. 2009, Pubmed
Runcie, Genetics of gene expression responses to temperature stress in a sea urchin gene network. 2012, Pubmed , Echinobase
Ruscetti, Transforming growth factor-beta and the immune system. 1991, Pubmed
Shannon, Cytoscape: a software environment for integrated models of biomolecular interaction networks. 2003, Pubmed
Simão, BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs. 2015, Pubmed
Szklarczyk, The STRING database in 2011: functional interaction networks of proteins, globally integrated and scored. 2011, Pubmed
Tarazona, Differential expression in RNA-seq: a matter of depth. 2011, Pubmed
Vergara-Amado, Differential expression of stress candidate genes for thermal tolerance in the sea urchin Loxechinus albus. 2017, Pubmed , Echinobase
Villalpando, TvZNF1 is a C2H2 zinc finger protein of Trichomonas vaginalis. 2017, Pubmed
Wang, Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves. 2010, Pubmed
Wang, Parental Effect of Long Acclimatization on Thermal Tolerance of Juvenile Sea Cucumber Apostichopus japonicus. 2015, Pubmed , Echinobase
Yan, De novo transcriptome sequencing and gene expression profiling of spinach (Spinacia oleracea L.) leaves under heat stress. 2016, Pubmed
Yang, Effect of high temperature on immune response of grass carp (Ctenopharyngodon idellus) by transcriptome analysis. 2016, Pubmed
Yang, Transcriptome profiling of grass carp (Ctenopharyngodon idellus) infected with Aeromonas hydrophila. 2016, Pubmed
Yu, Impact of water temperature on the growth and fatty acid profiles of juvenile sea cucumber Apostichopus japonicus (Selenka). 2016, Pubmed , Echinobase
Zhan, A novel p38 MAPK gene in the sea cucumber Apostichopus japonicus (Ajp38) is associated with the immune response to pathogenic challenge. 2018, Pubmed , Echinobase
Zhang, Characterization of two heat shock proteins (Hsp70/Hsc70) from grass carp (Ctenopharyngodon idella): evidence for their differential gene expression, protein synthesis and secretion in LPS-challenged peripheral blood lymphocytes. 2011, Pubmed
Zhang, A greedy algorithm for aligning DNA sequences. 2000, Pubmed
Zhang, Effects of long-term elevated temperature on covering, sheltering and righting behaviors of the sea urchin Strongylocentrotus intermedius. 2017, Pubmed , Echinobase
Zito, Expression of univin, a TGF-beta growth factor, requires ectoderm-ECM interaction and promotes skeletal growth in the sea urchin embryo. 2003, Pubmed , Echinobase


	FIGURE 2. Summary statistics of the S. intermedius transcriptomes. (A) Summary statistics of the RNA-Seq data and transcriptome assembly results. (B) Pearson correlation coefficients between samples used in the current study. (C) Venn diagram showing NR, COG, KEGG, Swiss-Prot, and InterPro.
	FIGURE 3. Frequency of identified (A) single nucleotide polymorphisms and (B) simple sequence repeats among the transcriptome libraries Si_TT0 (n = 2) and Si_TT2 (n = 2).

	FIGURE 5. Functional annotation of DEGs of Si_TT2 vs. Si_TT0 in S. intermedius. (A) The most enriched GO terms of DEGs of S. intermedius Si_TT0and S. intermedius Si_TT2. The Y-axis represents the categories of annotated DEGs, and the X-axis represents the number of DEGs. (B) The top 20 enriched KEGG terms of DEGs of Si_TT2 vs. Si_TT0 in S. intermedius. The Y-axis represents the KEGG pathway, and the X-axis represents the enrichment factor. Dot size indicates the number of DEGs in the pathway. Dot colors corresponds to different Q-values.
	FIGURE 6. Differentially expressed genes interactive network prediction. (A) Interactive network prediction of all DEGs. (B) Interactive network prediction of MIB2. (C) Interactive network prediction of heat shock protein 90.
	FIGURE 7. Transcription factor families identified in S. intermedius Si_TT0 and S. intermedius Si_TT2 transcriptomes. (A) The distribution of all of the identified transcription factors. The number of each transcription factor is shown. (B) The distribution of differentially expressed transcription factors. The number and the expression of each differentially expressed transcription factor are shown.