Lin WC et al. (2017), Development and Characterisation of the Imiquim...

ECB-ART-47122

Polymers (Basel) 2017 Nov 05;911:. doi: 10.3390/polym9110579.

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Development and Characterisation of the Imiquimod Poly(2-(2-methoxyethoxy)ethyl Methacrylate) Hydrogel Dressing for Keloid Therapy.

Lin WC , Liou SH , Kotsuchibashi Y .

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The imiquimod-poly(2-(2-methoxyethoxy)ethyl methacrylate) hydrogel (poly(MEO₂MA) hydrogel) dressing was developed for the keloid therapy application. Four groups of the hydrogels, including the imiquimod-poly(MEO₂MA) hydrogel, crosslinked with 0.2 mol %, 0.4 mol %, 0.6 mol %, and 0.8 mol % of di(ethylene glycol) dimethacrylate cross-linker (DEGDMA), were synthesised and characterised for fabricating the imiquimod-poly(MEO₂MA) hydrogel pad. The lower critical solution temperature (LCST) of the poly(MEO₂MA) hydrogel was measured at approximately 28 °C and was used as a trigger to control the imiquimod loading and release. The loaded amounts of the imiquimod in the poly(MEO₂MA) hydrogel, crosslinked with 0.2 mol % and 0.8 mol % of DEGDMA, were about 27.4 μg and 14.1 μg per 1 mm³ of the hydrogel, respectively. The imiquimod-release profiles of two samples were characterised in a phosphate buffered saline (PBS) solution at 37 °C and the released imiquimod amount were about 45% and 46% of the total loaded imiquimod. The Cell Counting Kit-8 (CCK-8) assay was utilised to analyse the cell viability of keloid fibroblasts cultured on the samples of imiquimod-poly(MEO₂MA) hydrogel, crosslinked with 0.2 mol % and 0.8 mol % of DEGDMA. There was around a 34% decrease of the cell viabilities after 2 days, compared with the pure-poly(MEO₂MA) hydrogel samples. Therefore, the developed imiquimod-poly(MEO₂MA) hydrogel dressing can affect the proliferation of keloid fibroblasts. It should be possible to utilise the hydrogel dressing for the keloid therapy application.

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Genes referenced: LOC115919910

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References [+] :

Ali, Polyethylene glycol as an alternative polymer solvent for nanoparticle preparation. 2013, Pubmed

	Figure 1. Schematic illustration of our purposed imiquimod-poly(MEO2MA) hydrogel-based treatment for keloid therapy. LCST: lower critical solution temperature.
	Figure 2. The synthesis of the poly(2-(2-methoxyethoxy)ethyl methacrylate) hydrogel (poly(MEO2MA) hydrogel) by redox polymerisation. DEGDMA (cross-linker; CL): di(ethylene glycol) dimethacrylate; APS: ammonium persulphate; TEMED: N,N,N′,N′-tetramethylethylenediamine.
	Figure 3. The illustration of the casting process to fabricate poly(MEO2MA) hydrogel film.
	Figure 4. (a) Photo images of the fabricated poly(MEO2MA) hydrogel film by using the casting approach and (b) the disc-shape of poly(MEO2MA) hydrogel samples with four different molar feed ratios of cross-linker.
	Figure 5. (a) Optical transmittance of poly(MEO2MA) hydrogel samples were measured as a function of temperature at 500 nm with the scan rate 2 °C min−1. (b–d) The transmittance changes of poly(MEO2MA) hydrogel pads were characterised in Milli-Q water, phosphate buffered saline (PBS), and dimethylformamid (DMF) solution under the temperatures of 4 °C, 25 °C, and 37 °C, respectively.
	Figure 6. Dynamic swelling profiles of poly(MEO2MA) hydrogel samples measured in DMF, Milli-Q water, and PBS at constant temperature of 4 °C for 72 h, respectively.
	Figure 7. The swelling ratios of poly(MEO2MA) hydrogel, crosslinked with 0.2 mol % of DEGDMA samples, within 12 h in Milli-Q water and DMF solution.
	Figure 8. (a) Deswelling ratios of poly(MEO2MA) hydrogel samples were measured as a function of the temperature in Milli-Q water and PBS solution. (b) Optical images of the poly(MEO2MA) hydrogel, crosslinked with 0.2 mol % of DEGDMA, corresponding to the temperature changes between 4 °C and 37 °C.
	Figure 9. The bar chart presents the deswelling ratios of poly(MEO2MA) hydrogel, crosslinked with 0.2 mol % and 0.8 mol % of DEGDMA at 37 °C in PBS solution and Milli-Q water, respectively.
	Figure 10. (a) The characterisation of imiquimod-release profiles from the samples of the imiquimod-poly(MEO2MA) hydrogel, crosslinked with 0.2 mol % and 0.8 mol % of DEGDMA in PBS solution at 37 °C. (b) The details of imiquimod-release profiles in the first 24 h.
	Figure 11. The cell viability was measured from keloid fibroblasts cultured on the polystyrene dish, pure-poly(MEO2MA) hydrogel, and imiquimod-poly(MEO2MA) hydrogel, crosslinked with 0.2 mol % and 0.8 mol % of DEGDMA by using Cell Counting Kit-8 (CCK-8) assay and enzyme-linked immunosorbent assay (ELISA).