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	Figure 1. cDNA sequence and deduced amino acid sequence of OXYL. The asterisk (19Asp) indicates the first N-terminal amino acid of the mature lectin (yellow). The amino acid sequence identified by Edman degradation is underlined.
	Figure 2. Panel A: Multiple sequence alignment of the full-length OXYL precursor and homologous sequences from other crinoid species. Histogram bars represent amino acid conservation in each position of the alignment. The signal peptide region is indicated in a red box. The 8 residues typically conserved in C1qDC proteins are marked with an asterisk. Panel B: Bayesian phylogenetic tree of OXYL-like sequences. Multiple sequences obtained from the same species are indicated with progressive numbers. The tree was rooted by using the C1qDC protein sequences from the genome of the sea urchin Strongylocentrotus purpuratus (indicated with GenBank accession codes, starting with “XP_”) and the three C1q domains from the two C1qDC proteins identified in the A. japonica transcriptome, but unrelated to OXYL. For simplicity’s sake, outgroup sequences have been collapsed in cartoons. Numbers close to each node represent posterior probabilities. The OXYL clade is indicated with a blue background. Panel C: neighbor joining tree of all C1qDC sequences from echinoderms, based on the multiple sequence alignment of the C1q domain (see materials and methods for details). The OXYL clade, supported by bootstrap value = 94, is indicated with a blue background. Each sequence is designated with a six letter code, indicating the first three letters of the genus and species name (see materials and methods for a complete list of sequences).
	Figure 3. Multimeric structure of OXYL by using analytical ultracetrifugation. Distribution of sedimentation coefficient c(s20,w) by sedimentation velocity AUC. Calculated c(s) was plotted versus s20,w the sedimentation coefficients corrected to 20°C in water. a: very likely come from salt and/or buffer, b: 3.28 S (37 kDa, dimer), c: 4.88 S (65.8 kDa, tetramer), d: 7.25 S (121 kDa, octemer), and e: 9.19 S (178 kDa, dodecamer). The experiment was performed with 0.4 mg/mL protein.
	Figure 4. Detection of OXYL by antiserum. A. japonica arm extract (crude extract) and purified lectin (OXYL) were separated by SDS-PAGE and transferred to a membrane. OXYL (asterisk) was detected by peroxidase staining of HRP-conjugated goat anti-rabbit IgG raised against OXYL and by Coomassie Brilliant Blue R-250. Numbers at left (pre-stained) and right: molecular mass (×1000) standard.
	Figure 5. Localization of OXYL in A. japonica. Paraffin-embedded serial sections were stained by hematoxylin-eosin and anti-OXYL rabbit antiserum (A–N) and pre-immune rabbit serum (O–Q) followed by FITC-conjugated secondary anti-rabbit IgG goat antibody, and observed by fluorescence microscopy. Detections: FITC (A,D,E,H,J,M,,P), DAPI (C,D,G,H,K,N,,Q) and hematoxylin-eosin staining (B,F,I,L,O). The square in panel B was zoomed-in E-H. Scale bars: 100 μm (white) and 300 μm (black), respectively.
	Figure 6. Glycan-binding properties of OXYL. Fluorescence-labeled OXYL was subjected to glycan array analysis using glycochip, where 8 glycan structures were immobilized. Fluorescence signals for the 8 glycans (listed in Table 1) are represented as signal intensities.
	Figure 7. Agglutination of Pseudomonas aeruginosa by OXYL. Two micrograms of OXYL were administrated to the bacteria (A–D,a–d), which were observed by fluorescence microscopy (A–E; λex/em = 550/566 nm) and phase-contrast microscopy (a–e). Ten mM LacNAc (B,b), lactose (C,c) or 0.5 mg/mL lipopolysaccharide (D,d) were added in co-presence with OXYL. E and e are negative controls without OXYL. The bar indicates 100 μm.
	Figure 9. Binding and growth effect of OXYL to cancer cells. A: HiLyte Fluoro 555-conjugated OXYL (10 μg per cells) was administrated to BT-474 (a,e), MCF-7 (b,f), T47D (c,g), and HeLa (d,h) without (a–d) or with (e–h) LacNAc (10 mM). B: Cell viability of OXYL against four types of cancer cells. Cells were treated with OXYL at various concentrations (0–100 μg/mL) for 48 h, and viability was determined by WST-8 assay. Values for BT-474, MCF-7, T47D, and HeLa are shown by fine dotted, hatched, rough dotted and mesh bars, respectively.
	Figure 10. Effects of OXYL on phosphorylation and expression levels of p38 and caspase-3 in the BT-474 cell line. Cells (4 × 105 in each experiment) were treated with various concentrations of OXYL (0–100 µg/mL), and activation levels were evaluated by western blotting of lysates in absence (−) and presence (+) of LacNAc (10 mM). Experiments were performed in triplicates.

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