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Anti-Vasculogenic Activity of a Polysaccharide Derived from Brittle Star via Inhibition of VEGF, Paxillin and MMP-9.
Baharara J
,
Amini E
,
Musavi M
.
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Background: Bioactive compounds such as terpenoids, chondroitin sulfate, and polysaccharides with added value can be found in prestine marine creatures. These compounds often do have highly valuable therapeutic applications such as being antioxidant, antitumorogenic, anti-inflammatory and anti-angiogenic. For the latter, varieties of angiogenesis factors can suppress this issue within the bodily tissues. Objectives: The anti-angiogenic and anti-metastatic capacity of a polysaccharide derived from brittle star was investigated. Material and Methods: The anti-proliferative effect of derived polysaccharide on umbilical vein endothelial cells (HUVEC) was measured using MTT (dimethyl thiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. The anti-angiogenic effect of the isolated polysaccharide was examined by Chorioallantoic membrane (CAM) assay. The transcriptional expression of VEGF (Vascular Endothelial Growth Factor) was evaluated by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The anti-metastatic activity was investigated via scratch-wound healing assay. The levels of Paxillin and Matrix Metalloproteinase-9 (MMP-9) expression were analyzed by RT-PCR. Statistical analysis and mean comparisons (p< 0.05) were carried out by SPSS 16. Results: Our results elucidated that the brittle star isolated polysaccharide exerted a dose dependent cytotoxic effect on the HUVEC endothelial cells. The CAM assay exhibited potent anti-angiogenic activity in vivo. The RT-PCR analysis showed that the extracted polysaccharide (40, 60 µg.mL-1) down-regulated the VEGF expression. Further, the diminished attachment of endothelial cells demonstrated that the anti-invasiveness of the derived polysaccharide (25, 50 µg.mL-1) was administrated via down-regulation of paxillin and MMP-9 mRNA expression. Conclusions: Taken together, these results indicated that the polysaccharide extracted from brittle star was able to decrease the viability of the HUVEC cells, to suppress angiogenesis, and possibly act as a natural anti-angiogenic and anti-metastatic marine organic compound against angiogenesis related pathologies.
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,
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Blunt,
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Cao,
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Deakin,
Distinct roles for paxillin and Hic-5 in regulating breast cancer cell morphology, invasion, and metastasis.
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Edwards,
Animal models for anti-angiogenic therapy in endometriosis.
2013,
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Elice,
Side effects of anti-angiogenic drugs.
2012,
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Fan,
Angiogenesis: from plants to blood vessels.
2006,
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Ferrara,
Angiogenesis as a therapeutic target.
2005,
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Folkman,
Angiogenesis: an organizing principle for drug discovery?
2007,
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Guerra Dore,
Antiangiogenic activity and direct antitumor effect from a sulfated polysaccharide isolated from seaweed.
2013,
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He,
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2012,
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Iiizumi,
Drug development against metastasis-related genes and their pathways: a rationale for cancer therapy.
2008,
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Kang,
Soybean saponin inhibits tumor cell metastasis by modulating expressions of MMP-2, MMP-9 and TIMP- 2.
2008,
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Lieu,
Beyond VEGF: inhibition of the fibroblast growth factor pathway and antiangiogenesis.
2011,
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Liu,
Fucoidan extract derived from Undaria pinnatifida inhibits angiogenesis by human umbilical vein endothelial cells.
2012,
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Lu,
A polysaccharide fraction of adlay seed (Coixlachryma-jobi L.) induces apoptosis in human non-small cell lung cancer A549 cells.
2013,
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Miller,
Paclitaxel plus bevacizumab versus paclitaxel alone for metastatic breast cancer.
2007,
Pubmed
Motzer,
Sunitinib versus interferon alfa in metastatic renal-cell carcinoma.
2007,
Pubmed
Myron,
Fucosylated chondroitin sulfate diversity in sea cucumbers: a review.
2014,
Pubmed
,
Echinobase
Reichert,
Development trends for new cancer therapeutics and vaccines.
2008,
Pubmed
Sandler,
Paclitaxel-carboplatin alone or with bevacizumab for non-small-cell lung cancer.
2006,
Pubmed
Senthilkumar,
Antiangiogenic effects of marine sponge derived compounds on cancer.
2013,
Pubmed
Thakur,
Marine molecular biology: an emerging field of biological sciences.
2008,
Pubmed
Xin,
The inhibitory effect of a polysaccharide from Codonopsis pilosula on tumor growth and metastasis in vitro.
2012,
Pubmed
Yang,
Anti-angiogenic effects and mechanisms of polysaccharides from Antrodia cinnamomea with different molecular weights.
2009,
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Zhang,
Modified apple polysaccharides suppress the migration and invasion of colorectal cancer cells induced by lipopolysaccharide.
2013,
Pubmed
Zheng,
Huaier polysaccharides suppresses hepatocarcinoma MHCC97-H cell metastasis via inactivation of EMT and AEG-1 pathway.
2014,
Pubmed
Zong,
Anti-metastatic and anti-angiogenic activities of sulfated polysaccharide of Sepiella maindroni ink.
2013,
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Zong,
Anticancer polysaccharides from natural resources: a review of recent research.
2012,
Pubmed
