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Lipids Health Dis
2018 Mar 20;171:55. doi: 10.1186/s12944-018-0710-z.
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Palmitoleic acid (16:1n7) increases oxygen consumption, fatty acid oxidation and ATP content in white adipocytes.
Cruz MM
,
Lopes AB
,
Crisma AR
,
de Sá RCC
,
Kuwabara WMT
,
Curi R
,
de Andrade PBM
,
Alonso-Vale MIC
.
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BACKGROUND: We have recently demonstrated that palmitoleic acid (16:1n7) increases lipolysis, glucose uptake and glucose utilization for energy production in white adipose cells. In the present study, we tested the hypothesis that palmitoleic acid modulates bioenergetic activity in white adipocytes.
METHODS: For this, 3 T3-L1 pre-adipocytes were differentiated into mature adipocytes in the presence (or absence) of palmitic (16:0) or palmitoleic (16:1n7) acid at 100 or 200 μM. The following parameters were evaluated: lipolysis, lipogenesis, fatty acid (FA) oxidation, ATP content, oxygen consumption, mitochondrial mass, citrate synthase activity and protein content of mitochondrial oxidative phosphorylation (OXPHOS) complexes.
RESULTS: Treatment with 16:1n7 during 9 days raised basal and isoproterenol-stimulated lipolysis, FA incorporation into triacylglycerol (TAG), FA oxidation, oxygen consumption, protein expression of subunits representing OXPHOS complex II, III, and V and intracellular ATP content. These effects were not observed in adipocytes treated with 16:0.
CONCLUSIONS: Palmitoleic acid, by concerted action on lipolysis, FA esterification, mitochondrial FA oxidation, oxygen consumption and ATP content, does enhance white adipocyte energy expenditure and may act as local hormone.
Fig. 1. Basal (a) and isoproterenol-stimulated (b) lipolysis measuring by release of glycerol (nanomoles per 106 cells). c mRNA levels of Pnpla2 gene (arbitrary units). Experiments performed in differentiated 3 T3-L1 adipocytes treated for 9 days with vehicle, palmitic acid (16:0, 100 μM) or palmitoleic acid (16:1n7, 100 μM). Results are means ± SEM. * P < 0.05 16:1n7 vs. all groups. The results are the average of 3 independent experiments (n = 4/experiment)
Fig. 2. a [1-14C]-palmitate incorporation into TAG (nanomoles of incorporated [1-14C]-palmitate per 106 cells) and b mRNA levels of aP2 (arbitrary units). Experiments performed in differentiated 3 T3-L1 adipocytes treated for 9 days with vehicle, palmitic acid (16:0, 100 μM) or palmitoleic acid (16:1n7, 100 μM). Results are means ± SEM. * P < 0.05 16:1n7 vs. all groups. The results are the average of 3 independent experiments (n = 6/experiment)
Fig. 8. Treatment with 16:1n7 for 9 days stimulated both lipolysis (1) and FFA re-esterification (4) in 3 T3-L1 adipocytes. Raised lipolysis leads to FFA (2) and glycerol release. FFA follow different fates (3): re-esterification into TAG (4), plasma (5) and mitochondria oxidation (6). 16:1n7 raised mitochondrial parameters such as FAO (6), oxygen consumption (7) and ATP generation (8). In addition, ETC protein expression is also elevated by 16:n7: complex II (9), complex III (10) and ATP synthase (11). ATP augmentation contributes to reesterification (12). Altogether, 16:1n7 enhances WAT futile cycle (lipolysis-re-esterification cycle) as well as mitochondria bioenergetics
Ahmadian,
The skinny on fat: lipolysis and fatty acid utilization in adipocytes.
2009, Pubmed
Ahmadian,
The skinny on fat: lipolysis and fatty acid utilization in adipocytes.
2009,
Pubmed
Alp,
Activities of citrate synthase and NAD+-linked and NADP+-linked isocitrate dehydrogenase in muscle from vertebrates and invertebrates.
1976,
Pubmed
Amengual,
Induction of carnitine palmitoyl transferase 1 and fatty acid oxidation by retinoic acid in HepG2 cells.
2012,
Pubmed
Baht,
Comparison of triacylglycerol synthesis in rat brown and white adipocytes. Effects of hypothyroidism and streptozotocin-diabetes on enzyme activities and metabolic fluxes.
1988,
Pubmed
Baldwin,
Metabolic functions affecting the contribution of adipose tissue to total energy expenditure.
1970,
Pubmed
Bolsoni-Lopes,
Palmitoleic acid (n-7) increases white adipocyte lipolysis and lipase content in a PPARα-dependent manner.
2013,
Pubmed
Bolsoni-Lopes,
Palmitoleic acid (n-7) increases white adipocytes GLUT4 content and glucose uptake in association with AMPK activation.
2014,
Pubmed
,
Echinobase
Böttcher,
Decreased white fat cell thermogenesis in obese individuals.
1997,
Pubmed
Cao,
Identification of a lipokine, a lipid hormone linking adipose tissue to systemic metabolism.
2008,
Pubmed
Carracedo,
Cancer metabolism: fatty acid oxidation in the limelight.
2013,
Pubmed
Carrière,
Inhibition of preadipocyte proliferation by mitochondrial reactive oxygen species.
2003,
Pubmed
Crescenzo,
Skeletal muscle mitochondrial energetic efficiency and aging.
2015,
Pubmed
De Pauw,
Mitochondrial (dys)function in adipocyte (de)differentiation and systemic metabolic alterations.
2009,
Pubmed
Diakogiannaki,
Mechanisms involved in the cytotoxic and cytoprotective actions of saturated versus monounsaturated long-chain fatty acids in pancreatic beta-cells.
2007,
Pubmed
Dib,
LXRα fuels fatty acid-stimulated oxygen consumption in white adipocytes.
2014,
Pubmed
Dimopoulos,
Differential effects of palmitate and palmitoleate on insulin action and glucose utilization in rat L6 skeletal muscle cells.
2006,
Pubmed
Duchen,
Mitochondria in health and disease: perspectives on a new mitochondrial biology.
2004,
Pubmed
Flachs,
Stimulation of mitochondrial oxidative capacity in white fat independent of UCP1: a key to lean phenotype.
2013,
Pubmed
Forest,
Fatty acid recycling in adipocytes: a role for glyceroneogenesis and phosphoenolpyruvate carboxykinase.
2003,
Pubmed
Frayn,
Fatty acid-induced mitochondrial uncoupling in adipocytes is not a promising target for treatment of insulin resistance unless adipocyte oxidative capacity is increased.
2008,
Pubmed
Gao,
Mitochondrial dysfunction is induced by high levels of glucose and free fatty acids in 3T3-L1 adipocytes.
2010,
Pubmed
Garlid,
Mechanism of uncoupling protein action.
2001,
Pubmed
Gauthier,
AMP-activated protein kinase is activated as a consequence of lipolysis in the adipocyte: potential mechanism and physiological relevance.
2008,
Pubmed
Geisler,
Targeting energy expenditure via fuel switching and beyond.
2011,
Pubmed
Harms,
Brown and beige fat: development, function and therapeutic potential.
2013,
Pubmed
Harper,
Factors affecting fatty acid oxidation in fat cells isolated from rat white adipose tissue.
1976,
Pubmed
Heinonen,
Impaired Mitochondrial Biogenesis in Adipose Tissue in Acquired Obesity.
2015,
Pubmed
Hodson,
Is there something special about palmitoleate?
2013,
Pubmed
Jensen,
Lipolysis: contribution from regional fat.
1997,
Pubmed
Kaaman,
Strong association between mitochondrial DNA copy number and lipogenesis in human white adipose tissue.
2007,
Pubmed
Kita,
Possible role of mitochondrial remodelling on cellular triacylglycerol accumulation.
2009,
Pubmed
Klaman,
Increased energy expenditure, decreased adiposity, and tissue-specific insulin sensitivity in protein-tyrosine phosphatase 1B-deficient mice.
2000,
Pubmed
Lanza,
Functional assessment of isolated mitochondria in vitro.
2009,
Pubmed
Liu,
Activation of AMP-activated protein kinase signaling pathway by adiponectin and insulin in mouse adipocytes: requirement of acyl-CoA synthetases FATP1 and Acsl1 and association with an elevation in AMP/ATP ratio.
2010,
Pubmed
Lobo,
Functional analysis of long-chain acyl-CoA synthetase 1 in 3T3-L1 adipocytes.
2009,
Pubmed
Maassen,
Fatty acid-induced mitochondrial uncoupling in adipocytes as a key protective factor against insulin resistance and beta cell dysfunction: a new concept in the pathogenesis of obesity-associated type 2 diabetes mellitus.
2007,
Pubmed
Mercader,
All-trans retinoic acid increases oxidative metabolism in mature adipocytes.
2007,
Pubmed
Morgan,
Unsaturated fatty acids as cytoprotective agents in the pancreatic beta-cell.
2010,
Pubmed
Obanda,
Modulation of cellular insulin signaling and PTP1B effects by lipid metabolites in skeletal muscle cells.
2013,
Pubmed
Park,
Phosphorylation of C/EBPbeta at a consensus extracellular signal-regulated kinase/glycogen synthase kinase 3 site is required for the induction of adiponectin gene expression during the differentiation of mouse fibroblasts into adipocytes.
2004,
Pubmed
Reidy,
Accelerated substrate cycling: a new energy-wasting role for leptin in vivo.
2002,
Pubmed
Rogge,
The role of impaired mitochondrial lipid oxidation in obesity.
2009,
Pubmed
Roman,
Brown adipose tissue and novel therapeutic approaches to treat metabolic disorders.
2015,
Pubmed
Rousset,
The biology of mitochondrial uncoupling proteins.
2004,
Pubmed
Ruge,
Fasted to fed trafficking of Fatty acids in human adipose tissue reveals a novel regulatory step for enhanced fat storage.
2009,
Pubmed
Sethi,
Thematic review series: adipocyte biology. Adipose tissue function and plasticity orchestrate nutritional adaptation.
2007,
Pubmed
Shen,
R-alpha-lipoic acid and acetyl-L-carnitine complementarily promote mitochondrial biogenesis in murine 3T3-L1 adipocytes.
2008,
Pubmed
Surwit,
Diet-induced changes in uncoupling proteins in obesity-prone and obesity-resistant strains of mice.
1998,
Pubmed
Sutherland,
Exercise and adrenaline increase PGC-1{alpha} mRNA expression in rat adipose tissue.
2009,
Pubmed
Talbot,
Palmitoleic acid prevents palmitic acid-induced macrophage activation and consequent p38 MAPK-mediated skeletal muscle insulin resistance.
2014,
Pubmed
Tourniaire,
All-trans retinoic acid induces oxidative phosphorylation and mitochondria biogenesis in adipocytes.
2015,
Pubmed
Vallerand,
Cold stress increases lipolysis, FFA Ra and TG/FFA cycling in humans.
1999,
Pubmed
Vankoningsloo,
Mitochondrial dysfunction induces triglyceride accumulation in 3T3-L1 cells: role of fatty acid beta-oxidation and glucose.
2005,
Pubmed
Wang,
Metabolic partitioning of endogenous fatty acid in adipocytes.
2003,
Pubmed
Wolfe,
Role of triglyceride-fatty acid cycle in controlling fat metabolism in humans during and after exercise.
1990,
Pubmed
Yang,
Chronic administration of palmitoleic acid reduces insulin resistance and hepatic lipid accumulation in KK-Ay Mice with genetic type 2 diabetes.
2011,
Pubmed
de Andrade,
Diabetes-associated mitochondrial DNA mutation A3243G impairs cellular metabolic pathways necessary for beta cell function.
2006,
Pubmed
de Sá,
Fish oil prevents changes induced by a high-fat diet on metabolism and adipokine secretion in mice subcutaneous and visceral adipocytes.
2016,
Pubmed
,
Echinobase
den Hartigh,
10E,12Z-conjugated linoleic acid impairs adipocyte triglyceride storage by enhancing fatty acid oxidation, lipolysis, and mitochondrial reactive oxygen species.
2013,
Pubmed