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Development
2018 Apr 04;1457:. doi: 10.1242/dev.160754.
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Polycomb group (PcG) proteins and Pax6 cooperate to inhibit in vivo reprogramming of the developing Drosophila eye.
Zhu J
,
Ordway AJ
,
Weber L
,
Buddika K
,
Kumar JP
.
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How different cells and tissues commit to and determine their fates has been a central question in developmental biology since the seminal embryological experiments conducted by Wilhelm Roux and Hans Driesch in sea urchins and frogs. Here, we demonstrate that Polycomb group (PcG) proteins maintain Drosophila eye specification by suppressing the activation of alternative fate choices. The loss of PcG in the developing eye results in a cellular reprogramming event in which the eye is redirected to a wing fate. This fate transformation occurs with either the individual loss of Polycomb proteins or the simultaneous reduction of the Pleiohomeotic repressive complex and Pax6. Interestingly, the requirement for retinal selector genes is limited to Pax6, as the removal of more downstream members does not lead to the eye-wing transformation. We also show that distinct PcG complexes are required during different developmental windows throughout eye formation. These findings build on earlier observations that the eye can be reprogrammed to initiate head epidermis, antennal and leg development.
Azpiazu,
Function and regulation of homothorax in the wing imaginal disc of Drosophila.
2000, Pubmed
Azpiazu,
Function and regulation of homothorax in the wing imaginal disc of Drosophila.
2000,
Pubmed
Bantignies,
Polycomb-dependent regulatory contacts between distant Hox loci in Drosophila.
2011,
Pubmed
Beck,
Maintenance of Hox gene expression patterns.
2010,
Pubmed
Beira,
The legacy of Drosophila imaginal discs.
2016,
Pubmed
Beuchle,
Polycomb group proteins and heritable silencing of Drosophila Hox genes.
2001,
Pubmed
Bliss,
TEMPERATURE CHARACTERISTICS FOR PREPUPAL DEVELOPMENT IN DROSOPHILA MELANOGASTER.
1926,
Pubmed
Classen,
A tumor suppressor activity of Drosophila Polycomb genes mediated by JAK-STAT signaling.
2009,
Pubmed
Coleman,
Causal role for inheritance of H3K27me3 in maintaining the OFF state of a Drosophila HOX gene.
2017,
Pubmed
Colombani,
Secreted peptide Dilp8 coordinates Drosophila tissue growth with developmental timing.
2012,
Pubmed
Czermin,
Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites.
2002,
Pubmed
Czerny,
twin of eyeless, a second Pax-6 gene of Drosophila, acts upstream of eyeless in the control of eye development.
1999,
Pubmed
Frey,
Molecular basis of PRC1 targeting to Polycomb response elements by PhoRC.
2016,
Pubmed
Garelli,
Imaginal discs secrete insulin-like peptide 8 to mediate plasticity of growth and maturation.
2012,
Pubmed
Glicksman,
Misregulation of homeotic gene expression in Drosophila larvae resulting from mutations at the extra sex combs locus.
1988,
Pubmed
Grimaud,
From genetics to epigenetics: the tale of Polycomb group and trithorax group genes.
2006,
Pubmed
Gutiérrez,
The role of the histone H2A ubiquitinase Sce in Polycomb repression.
2012,
Pubmed
Hadorn,
Transdetermination in cells.
1968,
Pubmed
Hauck,
Functional analysis of an eye specific enhancer of the eyeless gene in Drosophila.
1999,
Pubmed
Haynie,
Development of the eye-antenna imaginal disc and morphogenesis of the adult head in Drosophila melanogaster.
1986,
Pubmed
Herz,
Histone H3 lysine-to-methionine mutants as a paradigm to study chromatin signaling.
2014,
Pubmed
Janody,
A mosaic genetic screen reveals distinct roles for trithorax and polycomb group genes in Drosophila eye development.
2004,
Pubmed
Kassis,
Polycomb and Trithorax Group Genes in Drosophila.
2017,
Pubmed
Katsuyama,
Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification.
2005,
Pubmed
Kim,
The pleiohomeotic gene is required for maintaining expression of genes functioning in ventral appendage formation in Drosophila melanogaster.
2008,
Pubmed
Klymenko,
A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities.
2006,
Pubmed
Kumar,
EGF receptor and Notch signaling act upstream of Eyeless/Pax6 to control eye specification.
2001,
Pubmed
Kumar,
Retinal determination the beginning of eye development.
2010,
Pubmed
Kurata,
Notch signaling and the determination of appendage identity.
2000,
Pubmed
Laprell,
Propagation of Polycomb-repressed chromatin requires sequence-specific recruitment to DNA.
2017,
Pubmed
Lee,
Suppression of Polycomb group proteins by JNK signalling induces transdetermination in Drosophila imaginal discs.
2005,
Pubmed
Lewis,
A gene complex controlling segmentation in Drosophila.
1978,
Pubmed
Loubière,
Coordinate redeployment of PRC1 proteins suppresses tumor formation during Drosophila development.
2016,
Pubmed
Ma,
Wingless and patched are negative regulators of the morphogenetic furrow and can affect tissue polarity in the developing Drosophila compound eye.
1995,
Pubmed
Martinez,
Polyhomeotic has a tumor suppressor activity mediated by repression of Notch signaling.
2009,
Pubmed
Maves,
Wingless induces transdetermination in developing Drosophila imaginal discs.
1995,
Pubmed
Maves,
Cell determination and transdetermination in Drosophila imaginal discs.
1999,
Pubmed
Morrison,
Characterization of a dorsal-eye Gal4 Line in Drosophila.
2010,
Pubmed
Müller,
Histone methyltransferase activity of a Drosophila Polycomb group repressor complex.
2002,
Pubmed
Peng,
Transcription factor choice in the Hippo signaling pathway: homothorax and yorkie regulation of the microRNA bantam in the progenitor domain of the Drosophila eye imaginal disc.
2009,
Pubmed
Quiring,
Homology of the eyeless gene of Drosophila to the Small eye gene in mice and Aniridia in humans.
1994,
Pubmed
Salzer,
Identification of retinal transformation hot spots in developing Drosophila epithelia.
2010,
Pubmed
Scheuermann,
Histone H2A monoubiquitination and Polycomb repression: the missing pieces of the puzzle.
2012,
Pubmed
Schmid,
Transdetermination in the homeotic eye--antenna imaginal disc of Drosophila melanogaster.
1985,
Pubmed
Schubiger,
[State of determination of imaginal blastemas in embryos of Drosophila melanogaster as revealed by mixing experiments].
1969,
Pubmed
Schwartz,
Polycomb silencing mechanisms and the management of genomic programmes.
2007,
Pubmed
Shao,
Stabilization of chromatin structure by PRC1, a Polycomb complex.
1999,
Pubmed
Simon,
Mechanisms of polycomb gene silencing: knowns and unknowns.
2009,
Pubmed
Singh,
Eye suppression, a novel function of teashirt, requires Wingless signaling.
2002,
Pubmed
Spratford,
Dissection and immunostaining of imaginal discs from Drosophila melanogaster.
2014,
Pubmed
Steffen,
What are memories made of? How Polycomb and Trithorax proteins mediate epigenetic memory.
2014,
Pubmed
Struhl,
Genes controlling segmental specification in the Drosophila thorax.
1982,
Pubmed
Struhl,
A blastoderm fate map of compartments and segments of the Drosophila head.
1981,
Pubmed
Tiong,
Clonal analysis of segmental and compartmental homoeotic transformations in polycomb mutants of Drosophila melanogaster.
1990,
Pubmed
Treisman,
wingless inhibits morphogenetic furrow movement in the Drosophila eye disc.
1995,
Pubmed
Wang,
Hierarchical recruitment of polycomb group silencing complexes.
2004,
Pubmed
Weasner,
Competition among gene regulatory networks imposes order within the eye-antennal disc of Drosophila.
2013,
Pubmed
Weasner,
Retinal Expression of the Drosophila eyes absent Gene Is Controlled by Several Cooperatively Acting Cis-regulatory Elements.
2016,
Pubmed
Webber,
The relationship between long-range chromatin occupancy and polymerization of the Drosophila ETS family transcriptional repressor Yan.
2013,
Pubmed
Worley,
Regeneration and transdetermination in Drosophila imaginal discs.
2012,
Pubmed
Zhu,
Drosophila Pax6 promotes development of the entire eye-antennal disc, thereby ensuring proper adult head formation.
2017,
Pubmed