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Mar Drugs
2017 Nov 01;1511:. doi: 10.3390/md15110341.
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Cucumarioside A₂-2 Causes Macrophage Activation in Mouse Spleen.
Pislyagin EA
,
Manzhulo IV
,
Gorpenchenko TY
,
Dmitrenok PS
,
Avilov SA
,
Silchenko AS
,
Wang YM
,
Aminin DL
.
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The immunomodulatory effect of triterpene glycoside cucumarioside A₂-2 (CA₂-2), isolated from the Far Eastern sea cucumber Cucumaria japonica, was compared with lipopolysaccharide (LPS) on mouse spleen. It has been shown that the intraperitoneal (i.p.) glycoside administration leads to increased spleen macrophage activating markers iba-1, IL-1β, iNOs, ROS and NO formation, with additional change of macrophage phenotype to M1. The mass spectrometry profiles of peptide/protein were obtained using MALDI-TOF-MS on the different parts of spleen sections isolated by laser mircodissection techniques. It was found that i.p. stimulation of animals with CA₂-2 leads to marked changes in the intensity of the characteristic peaks of spleen peptides/proteins, primarily in red pulp.
Figure 4. Macrophage M1/M2 polarization and activation. Flow cytometry analyses of RAW 264.7 cells treated with CA2-2 (0.1 or 0.01 μM), following by LPS (1 μg/mL) and IFN-γ (20 ng/mL) for M1 polarization or IL-4 (20 ng/mL) for M2 polarization.
Figure 5. The process of cutting red and white pulp in the mouse spleen section by laser microdissection (Carl Zeiss PALM MicroBeam). Green circles indicate the white pulp excision area; blue circles indicate the red pulp excision area. It can be seen that some of the selected tissue sections have already been cut (A); The bottom of the tube cap with cut out and collected tissue sections (B); Representative MALDI-TOF mass spectra of the mouse spleen samples from white pulp (C) and red pulp (D); The protein profiles of mouse treated with CA2-2 are shown. The pseudo-gel view of representative mass spectra of two different mouse spleen samples (Control and LPS) with all individual spectra shown in a density scale, in the mass range from 5000 to 6000 Da (E) and corresponding mass spectra profile, showing significant differences between untreated and LPS treated animal samples (F).
Adamson,
Cytokine toxicity and induction of NO synthase activity in cultured mouse hepatocytes.
1993, Pubmed
Adamson,
Cytokine toxicity and induction of NO synthase activity in cultured mouse hepatocytes.
1993,
Pubmed
Aminin,
Glycosides from edible sea cucumbers stimulate macrophages via purinergic receptors.
2016,
Pubmed
,
Echinobase
Aminin,
Triterpene glycoside cucumarioside A(2)-2 from sea cucumber stimulates mouse immune cell adhesion, spreading, and motility.
2011,
Pubmed
,
Echinobase
Aminin,
Immunomodulatory Properties of Cucumariosides from the Edible Far-Eastern Holothurian Cucumaria japonica.
2001,
Pubmed
,
Echinobase
Cesta,
Normal structure, function, and histology of the spleen.
2006,
Pubmed
Fast,
Interferon-gamma, but not interferon-alpha beta, synergizes with tumor necrosis factor-alpha and lipid A in the induction of nitric oxide production by murine L929 cells.
1993,
Pubmed
Hirasawa,
Visualization of microglia in living tissues using Iba1-EGFP transgenic mice.
2005,
Pubmed
Imai,
A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage.
1996,
Pubmed
Kan,
Lung, spleen, and kidney are the major places for inducible nitric oxide synthase expression in endotoxic shock: role of p38 mitogen-activated protein kinase in signal transduction of inducible nitric oxide synthase expression.
2004,
Pubmed
Keisari,
IL-1, TNF-alpha and IL-2 production by peritoneal and spleen cells from Schistosoma mansoni infected mice and its potentiation by preimmunization with schistosomal antigens and immunostimulants.
1993,
Pubmed
Kollárová,
Production of IL-1 beta and IL-6 by adherent spleen cells after the stimulation with lipopolysaccharides from Salmonella enteritidis strain.
1996,
Pubmed
Kuypers,
Effects of intra-amniotic lipopolysaccharide and maternal betamethasone on brain inflammation in fetal sheep.
2013,
Pubmed
Martinez,
The M1 and M2 paradigm of macrophage activation: time for reassessment.
2014,
Pubmed
Pignol,
Effect of platelet-activating factor (PAF-acether) and its specific receptor antagonist, BN 52021, on interleukin 1 (IL1) release and synthesis by rat spleen adherent monocytes.
1987,
Pubmed
Pislyagin,
Determination of cucumarioside A₂-2 in mouse spleen by radiospectroscopy, MALDI-MS and MALDI-IMS.
2013,
Pubmed
Pislyagin,
Cucumarioside A2-2 causes changes in the morphology and proliferative activity in mouse spleen.
2016,
Pubmed
,
Echinobase
Pislyagin,
Interaction of holothurian triterpene glycoside with biomembranes of mouse immune cells.
2012,
Pubmed
,
Echinobase
Stuehr,
Mammalian nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide.
1985,
Pubmed
Vogel,
Stimulation of spleen cells and macrophages of C3H/HeJ mice by a lipid A precursor derived from Salmonella typhimurium.
1984,
Pubmed
Yang,
Allograft inflammatory factor-1 (AIF-1) is crucial for the survival and pro-inflammatory activity of macrophages.
2005,
Pubmed
