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Rouxs Arch Dev Biol
1994 Aug 01;2037-8:381-388. doi: 10.1007/BF00188686.
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Expression of a putative precursor mRNA for sperm-activating peptide I in accessory cells of the ovary in the sea urchin Hemicentrotus pulcherrimus.
Kinoh H
,
Shimizu T
,
Fujimoto H
,
Suzuki N
.
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A cDNA clone encoding egg jelly peptide, SAP-I (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly), was isolated from a Hemicentrotus pulcherrimus ovary cDNA library and its nucleotide sequence was determined. The cDNA was 1282 by long and an open reading frame predicted a protein of 334 amino acids containing 5 SAP-I and 7 SAP-I-like decapeptides, each separated by a single lysine residue. The cDNA hybridized to two species of mRNA (1.3 kb and 2.0 kb) from H. pulcherrimus ovaries. Northern blot analysis showed that the 1.3 kb transcripts appeared in ovaries collected from November to April and the 2.0 kb transcripts were detected only in ovaries collected in January. An expression study of the SAM precursor gene, by in situ hybridization with a non-radioactive RNA probe synthesized using the 1.3 kb cDNA as template, demonstrated that abundant SAM precursor transcripts were expressed in the accessory cells, but not in the growing oocytes.
Abe,
Immunocytochemical localization of an oviductal zona pellucida glycoprotein in the oviductal epithelium of the golden hamster.
1991, Pubmed
Abe,
Immunocytochemical localization of an oviductal zona pellucida glycoprotein in the oviductal epithelium of the golden hamster.
1991,
Pubmed
Abe,
The egg-jelly macromolecule, a fucose sulphate glycoconjugate, originates from the accessory cells of the ovary in the sea urchin Hemicentrotus pulcherrimus.
1992,
Pubmed
,
Echinobase
Abe,
Ultrastructural evidence for an association between an oviductal glycoprotein and the zona pellucida of the golden hamster egg.
1990,
Pubmed
Angerer,
Demonstration of tissue-specific gene expression by in situ hybridization.
1987,
Pubmed
Cathala,
A method for isolation of intact, translationally active ribonucleic acid.
1983,
Pubmed
Cavener,
Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates.
1987,
Pubmed
Garbers,
The amino acid sequence and chemical synthesis of speract and of speract analogues.
1982,
Pubmed
,
Echinobase
Henikoff,
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
1984,
Pubmed
Kozak,
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
1983,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Lee,
Modulation of the voltage-sensitive Na+/H+ exchange in sea urchin spermatozoa through membrane potential changes induced by the egg peptide speract.
1986,
Pubmed
,
Echinobase
Oikawa,
A glycoprotein of oviductal origin alters biochemical properties of the zona pellucida of hamster egg.
1988,
Pubmed
Peterson,
A simplification of the protein assay method of Lowry et al. which is more generally applicable.
1977,
Pubmed
Ramarao,
A single mRNA encodes multiple copies of the egg peptide speract.
1990,
Pubmed
,
Echinobase
Repaske,
A hydrogen ion flux mediates stimulation of respiratory activity by speract in sea urchin spermatozoa.
1983,
Pubmed
,
Echinobase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schackmann,
Alteration of intracellular [Ca2+] in sea urchin sperm by the egg peptide speract. Evidence that increased intracellular Ca2+ is coupled to Na+ entry and increased intracellular pH.
1986,
Pubmed
,
Echinobase
Schaefer,
Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides.
1985,
Pubmed
Schwartz,
The processing of peptide precursors. 'Proline-directed arginyl cleavage' and other monobasic processing mechanisms.
1986,
Pubmed
Steiner,
Insulin biosynthesis: evidence for a precursor.
1967,
Pubmed
Suzuki,
Some more speract derivatives associated with eggs of sea urchins, Pseudocentrotus depressus, Strongylocentrotus purpuratus, Hemicentrotus pulcherrimus and Anthocidaris crassispina.
1988,
Pubmed
,
Echinobase
Suzuki,
The relationship between amino acid sequences of sperm-activating peptides and the taxonomy of echinoids.
1992,
Pubmed
,
Echinobase
Suzuki,
Purification and the primary structure of sperm-activity peptides from the jelly coat of sea urchin eggs.
1981,
Pubmed
,
Echinobase
Suzuki,
Appearance of sperm activation factors in the ovary of the sea urchin Hemicentrotus pulcherrimus with maturation.
1982,
Pubmed
,
Echinobase
Tautz,
A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback.
1989,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Zhou,
Unique cholecystokinin peptides isolated from guinea pig intestine.
1985,
Pubmed
von Heijne,
A new method for predicting signal sequence cleavage sites.
1986,
Pubmed
