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Rouxs Arch Dev Biol
1987 Apr 01;1964:262-267. doi: 10.1007/BF00376351.
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Isolation of sea urchin embryo cell surface membranes on polycationic beads.
Helmly RB
,
Brown KM
.
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Blastula cell surface membranes of the sea urchin, Strongylocentrotus purpuratus, were isolated on polycationic beads by a method modified from Jacobson and Branton (1977) and Jacobson (1980). This study represents the first application of this procedure to an embryonic system. Embryo cells were attached to polylysine-coated polyacrylamide beads and lysed, leaving the embryo cell surface membranes still attached to the beads, and cytoplasmic particles were washed free of the exposed inner surfaces of the membranes. Cell surface membrane sheets were desorbed from the beads and collected by centrifugation. Approximately 8% and 5% of the cell surface membranes of dissociated embryo cells were recovered on the beads and in the membrane pellet, respectively. Specific activities of [3H]concanavalin A-binding and of the cell surface marker enzymes, alkaline phosphatase and Na+/K+ ATPase, were 16-, 19-, and 32-fold higher, respectively, in the cell surface membrane fraction than in the embryo cell homogenate. Membranes were relatively free of cytoplasmic contaminants as judged from electron micrographs and enzyme analysis. Activities in the membrane fraction of the cytoplasmic marker enzymes, cytochrome c oxidase, catalase, acid phosphatase, NADP- and NADPH-cytochrome c reductase, and acetylcholinesterase, were substantially less than homogenate levels. The entire procedure can be completed in 4 h. Since this cell surface membrane isolation technique relies only on the tendency of a negatively charged cell to adhere to a positively charged surface, it is less likely than most other methods to exhibit species and developmental stage specificity and should prove useful in the study of the developmental role of embryonic stage-specific membrane components.
APPELMANS,
Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver.
1955, Pubmed
APPELMANS,
Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver.
1955,
Pubmed
Akera,
Inhibition of brain sodium- and potassium-stimulated adenosine triphosphatase activity by chlorpromazine free radical.
1968,
Pubmed
BONTING,
Studies on sodium-potassium-activated adenosine triphosphatase. I. Quantitative distribution in several tissues of the cat.
1961,
Pubmed
Beaufay,
Analytical study of microsomes and isolated subcellular membranes from rat liver. 3. Subfractionation of the microsomal fraction by isopycnic and differential centrifugation in density gradients.
1974,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Brown,
[3H]serotonin binding to blastula, gastrula, prism, and pluteus sea urchin embryo cells.
1989,
Pubmed
,
Echinobase
Cestelli,
Isolation of the plasma membrane from sea urchin embryos.
1975,
Pubmed
,
Echinobase
Cohen,
Membrane isolation on polylysine-coated beads. Plasma membrane from HeLa cells.
1977,
Pubmed
De Duve,
Peroxisomes (microbodies and related particles).
1966,
Pubmed
Doré,
Acid phosphatase analysis in sea urchin eggs and blastulae.
1967,
Pubmed
,
Echinobase
Durán,
Chitin synthetase zymogen is attached to the yeast plasma membrane.
1975,
Pubmed
ELLMAN,
A new and rapid colorimetric determination of acetylcholinesterase activity.
1961,
Pubmed
Fink,
Three cell recognition changes accompany the ingression of sea urchin primary mesenchyme cells.
1985,
Pubmed
,
Echinobase
Giudice,
Reaggregation of mixtures of cells from different developmental stages of sea urchin embryos.
1969,
Pubmed
,
Echinobase
Giudice,
Reaggregation of dissociated cells of sea urchin embryos.
1970,
Pubmed
,
Echinobase
Gustafson,
Cellular movement and contact in sea urchin morphogenesis.
1967,
Pubmed
,
Echinobase
Hakomori,
Glycosphingolipids in cellular interaction, differentiation, and oncogenesis.
1981,
Pubmed
Hodges,
Purification of a plasma membrane-bound adenosine triphosphatase from plant roots.
1974,
Pubmed
Jacobson,
Imporved method for isolation of plasma membrane on cationic beads. Membranes from Dictyostelium discoideum.
1980,
Pubmed
Jacobson,
Isolation of plasma membrane from eukaryotic cells on polylysine-coated polyacrylamide beads.
1977,
Pubmed
Jacobson,
Plasma membrane: rapid isolation and exposure of the cytoplasmic surface by use of positively charged beads.
1977,
Pubmed
Kinoshita,
Isolation of human platelet plasma membranes with polylysine beads.
1979,
Pubmed
MAGGIO,
Cytochrome oxidase activity in the mitochondria of unfertilized and fertilized sea urchin eggs.
1959,
Pubmed
,
Echinobase
McClay,
Specificity of cell-cell interactions in sea urchin embryos. Appearance of new cell-surface determinants at gastrulation.
1977,
Pubmed
,
Echinobase
McClay,
Identification of four classes of cell surface antigens appearing at gastrulation in sea urchin embryos.
1978,
Pubmed
,
Echinobase
Oppenheimer,
Isolation of species-specific and stage-specific adhesion promoting component by disaggregation of intact sea urchin embryo cells.
1982,
Pubmed
,
Echinobase
Parish,
The isolation of plasma membranes from the cellular slime mold Dictyostelium discoideum using concanavalin A and Triton X-100.
1976,
Pubmed
Ribot,
Preparation of plasma membranes from fertilized sea urchin eggs.
1983,
Pubmed
,
Echinobase
Scarborough,
Isolation and characterization of Neurospora crassa plasma membranes.
1975,
Pubmed
Sottocasa,
An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study.
1967,
Pubmed
Walsh,
Preparation of inside-out vesicles of pig lymphocyte plasma membrane.
1976,
Pubmed
van der Meulen,
Isolation of chromaffin cell plasma membranes on polycationic beads.
1981,
Pubmed
