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The effect of trypsin on the fertilizability of sea urchin eggs was studied withParacentrotus lividus andPseudocentrotus depressus. The main effects were two reductions of fertilizability, with a transient increase intervening. The first decrease was probably caused by degradation of sperm-binding sites at the vitelline sheet and the second by degradation of binding sites on the plasma membrane.
Aketa,
On the sperm-egg bonding as the initial step of fertilization in the sea urchin.
1967, Pubmed,
Echinobase
Aketa,
On the sperm-egg bonding as the initial step of fertilization in the sea urchin.
1967,
Pubmed
,
Echinobase
Aketa,
Tryptic disruption of sperm-binding site of sea urchin egg surface.
1972,
Pubmed
,
Echinobase
Brown,
Enhancement of DNA synthesis in a mammalian cell-free system by trypsin treatment.
1974,
Pubmed
Glabe,
Isolation and characterization of the vitelline layer of sea urchin eggs.
1977,
Pubmed
,
Echinobase
HAGSTROM,
The action of trypsin and chymotrypsin on the sea urchin egg.
1954,
Pubmed
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Echinobase
Isaka,
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1970,
Pubmed
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Echinobase
Kane,
Hyalin release during normal sea urchin development and its replacement after removal at fertilization.
1973,
Pubmed
,
Echinobase
Kaplan,
Proteases as mitogens. The Effect of trypsin and pronase on mouse and human lymphocytes.
1974,
Pubmed
Pohjanpelto,
Proteases stimulate proliferation of human fibroblasts.
1977,
Pubmed
Schuel,
A trypsin-like proteinase localized in cortical granules isolated from unfertilized sea urchin eggs by zonal centrifugation. Role of the enzyme in fertilization.
1973,
Pubmed
,
Echinobase
Tsuzuki,
Purification of the sperm-binding factor from the egg of the sea urchin, Hemicentrotus pulcherrimus.
1976,
Pubmed
,
Echinobase
Vacquier,
Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs.
1977,
Pubmed
,
Echinobase
Vacquier,
Methods for quantitating sea urchin sperm-egg binding.
1973,
Pubmed
,
Echinobase
Vacquier,
Protease released from sea urchin eggs at fertilization alters the vitelline layer and aids in preventing polyspermy.
1973,
Pubmed
,
Echinobase
