Theodoro-Júnior OA , Righetti RF , Almeida-Reis R , Martins-Oliveira BT , Oliva LV , Prado CM , Saraiva-Romanholo BM , Leick EA , Pinheiro NM , Lobo YA , Martins MA , Oliva ML , Tibério IF .

	Figure 1. (A) Median and standard error (SE) of respiratory system elastance (Ers) for all experimental groups. * p < 0.05, compared with the saline (SAL), SAL-Enterolobium contortisiliquum trypsin inhibitor (EcTI) and elastase (ELA)-EcTI groups; (B) Median and SE of respiratory system resistance (Rrs) for all experimental groups. * p < 0.05, compared with the SAL-EcTI and ELA-EcTI groups; (C) Median and standard error of Raw for all experimental groups. * p < 0.05, compared with the SAL, SAL-EcTI and ELA-EcTI groups; (D) Mean and SE of Gtis for all experimental groups. There were no significant differences among the groups; (E) Median and SE of Htis for all experimental groups. * p < 0.05, compared with the SAL, SAL-EcTI and ELA-EcTI groups; (F) Photograph of the ENO and the mechanical evaluation: (1) Mylar bag for exhaled nitric oxide (ENO); (2) animal connected to a mechanical ventilator; (3) mechanical ventilator for small animals (Flexivent) and (4) nitric oxide filter.
	Figure 3. Mean and standard error of exhaled nitric oxide (ENO) for the four experimental groups. * p < 0.05, compared with the SAL and SAL-EcTI groups. ** p < 0.05, compared with the SAL and ELA groups.
	Figure 5. Inflammatory, remodeling and oxidative stress markers in the alveolar walls: photomicrographs of the results of the immunohistochemical analyses of the extracellular matrix inflammatory, remodeling and oxidative stress process in the alveolar walls to detect neutrophils, TNF-α, MMP-9, TIMP-1, iNOS and 8-iso-PGF2α. Magnification of 400×. All the following experimental groups are represented: SAL, ELA, SAL-EcTI and ELA-EcTI. We observed that the proteinase inhibitor, EcTI attenuated the cellular response induced by elastase. Scale bar = 30 µm.
	Figure 6. Inflammatory, remodeling and oxidative stress markers in the airway walls; photomicrographs of the results of the immunohistochemical analyses of the inflammatory, remodeling and oxidative stress process in the airway walls used to detect neutrophils, TNF-α, MMP-9, TIMP-1, iNOS and 8-iso-PGF2α. Magnification of 400×. All the following experimental groups are represented: SAL, ELA, SAL-EcTI and ELA-EcTI. We observed that the proteinase inhibitor EcTI attenuated the cellular response induced by elastase. Scale bar = 30 µm.
	Figure 7. Timeline of the experimental protocol. On the first day of the protocol, animals received intratracheal instillation of elastase or vehicle. 2 h after the intratracheal instillation, animals received an intraperitoneal injection of the treatment (EcTI or saline). On the fifteenth day, animals received the second dose of treatment (EcTI or saline). On the twenty-first day, animals received the last dose of saline or EcTI.

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