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Nanos is a translational regulator required for the survival and maintenance of primordial germ cells. In the sea urchin, Strongylocentrotus purpuratus (Sp), Nanos 2 mRNA is broadly transcribed but accumulates specifically in the small micromere (sMic) lineage, in part because of the 3''UTR element GNARLE leads to turnover in somatic cells but retention in the sMics. Here we found that the Nanos 2 protein is also selectively stabilized; it is initially translated throughout the embryo but turned over in the future somatic cells and retained only in the sMics, the future germ line in this animal. This differential stability of Nanos protein is dependent on the open reading frame (ORF), and is independent of the sumoylation and ubiquitylation pathways. Manipulation of the ORF indicates that 68 amino acids in the N terminus of the Nanos protein are essential for its stability in the sMics whereas a 45 amino acid element adjacent to the zinc fingers targets its degradation. Further, this regulation of Nanos protein is cell autonomous, following formation of the germ line. These results are paradigmatic for the unique presence of Nanos in the germ line by a combination of selective RNA retention, distinctive translational control mechanisms (Oulhen et al., 2013), and now also by defined Nanos protein stability.
Ahringer,
Control of the sperm-oocyte switch in Caenorhabditis elegans hermaphrodites by the fem-3 3' untranslated region.
1991, Pubmed
Ahringer,
Control of the sperm-oocyte switch in Caenorhabditis elegans hermaphrodites by the fem-3 3' untranslated region.
1991,
Pubmed Arrizabalaga,
A selective screen reveals discrete functional domains in Drosophila Nanos.
1999,
Pubmed Asaoka-Taguchi,
Maternal Pumilio acts together with Nanos in germline development in Drosophila embryos.
1999,
Pubmed Beer,
nanos3 maintains germline stem cells and expression of the conserved germline stem cell gene nanos2 in the zebrafish ovary.
2013,
Pubmed Bhat,
The posterior determinant gene nanos is required for the maintenance of the adult germline stem cells during Drosophila oogenesis.
1999,
Pubmed Broday,
The small ubiquitin-like modifier (SUMO) is required for gonadal and uterine-vulval morphogenesis in Caenorhabditis elegans.
2004,
Pubmed Cheers,
Rapid microinjection of fertilized eggs.
2004,
Pubmed
,
Echinobase Cho,
Cap-dependent translational inhibition establishes two opposing morphogen gradients in Drosophila embryos.
2006,
Pubmed Curtis,
A CCHC metal-binding domain in Nanos is essential for translational regulation.
1997,
Pubmed D'Agostino,
Translational repression restricts expression of the C. elegans Nanos homolog NOS-2 to the embryonic germline.
2006,
Pubmed Dai,
SUMO-1 of mud crab (Scylla paramamosain) in gametogenesis.
2012,
Pubmed Dalby,
Discrete sequence elements control posterior pole accumulation and translational repression of maternal cyclin B RNA in Drosophila.
1993,
Pubmed Draper,
nanos1 is required to maintain oocyte production in adult zebrafish.
2007,
Pubmed Edwards,
Structure of Pumilio reveals similarity between RNA and peptide binding motifs.
2001,
Pubmed Foltz,
Echinoderm eggs and embryos: procurement and culture.
2004,
Pubmed
,
Echinobase Forbes,
Nanos and Pumilio have critical roles in the development and function of Drosophila germline stem cells.
1998,
Pubmed Fujii,
Role of the nanos homolog during sea urchin development.
2009,
Pubmed
,
Echinobase Fukuda,
Ginkgolic acid inhibits protein SUMOylation by blocking formation of the E1-SUMO intermediate.
2009,
Pubmed Gavis,
Identification of cis-acting sequences that control nanos RNA localization.
1996,
Pubmed Gavis,
A conserved 90 nucleotide element mediates translational repression of nanos RNA.
1996,
Pubmed Geiss-Friedlander,
Concepts in sumoylation: a decade on.
2007,
Pubmed Gerber,
Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster.
2006,
Pubmed Gill,
Something about SUMO inhibits transcription.
2005,
Pubmed Goodwin,
Translational repression: not just a Puf of smoke.
2001,
Pubmed Gustafson,
Exogenous RNA is selectively retained in the small micromeres during sea urchin embryogenesis.
2010,
Pubmed
,
Echinobase Gustafson,
Post-translational regulation by gustavus contributes to selective Vasa protein accumulation in multipotent cells during embryogenesis.
2011,
Pubmed
,
Echinobase Haraguchi,
nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development.
2003,
Pubmed Hashimoto,
Crystal structure of zinc-finger domain of Nanos and its functional implications.
2010,
Pubmed Hashiyama,
Expression of genes involved in sumoylation in the Drosophila germline.
2009,
Pubmed Hayashi,
Nanos suppresses somatic cell fate in Drosophila germ line.
2004,
Pubmed Houston,
Germ plasm and molecular determinants of germ cell fate.
2000,
Pubmed Irish,
The Drosophila posterior-group gene nanos functions by repressing hunchback activity.
1989,
Pubmed Janic,
Ectopic expression of germline genes drives malignant brain tumor growth in Drosophila.
2010,
Pubmed Johnson,
Protein modification by SUMO.
2004,
Pubmed Julaton,
NANOS3 function in human germ cell development.
2011,
Pubmed Juliano,
Isolating specific embryonic cells of the sea urchin by FACS.
2014,
Pubmed
,
Echinobase Juliano,
Nanos functions to maintain the fate of the small micromere lineage in the sea urchin embryo.
2010,
Pubmed
,
Echinobase Kadyrova,
Translational control of maternal Cyclin B mRNA by Nanos in the Drosophila germline.
2007,
Pubmed Kloc,
The targeting of Xcat2 mRNA to the germinal granules depends on a cis-acting germinal granule localization element within the 3'UTR.
2000,
Pubmed Kobayashi,
Essential role of the posterior morphogen nanos for germline development in Drosophila.
1996,
Pubmed Komander,
The ubiquitin code.
2012,
Pubmed Köprunner,
A zebrafish nanos-related gene is essential for the development of primordial germ cells.
2001,
Pubmed Kraemer,
NANOS-3 and FBF proteins physically interact to control the sperm-oocyte switch in Caenorhabditis elegans.
1999,
Pubmed Lai,
Xenopus Nanos1 is required to prevent endoderm gene expression and apoptosis in primordial germ cells.
2012,
Pubmed Lai,
Repressive translational control in germ cells.
2013,
Pubmed Lai,
Nanos1 functions as a translational repressor in the Xenopus germline.
2011,
Pubmed Lee,
Molecular phylogenies and divergence times of sea urchin species of Strongylocentrotidae, Echinoida.
2003,
Pubmed
,
Echinobase Lomelí,
Emerging roles of the SUMO pathway in development.
2011,
Pubmed Luo,
Xenopus germline nanos1 is translationally repressed by a novel structure-based mechanism.
2011,
Pubmed Macdonald,
The Drosophila pumilio gene: an unusually long transcription unit and an unusual protein.
1992,
Pubmed Minokawa,
Expression patterns of four different regulatory genes that function during sea urchin development.
2004,
Pubmed
,
Echinobase Mosquera,
A mRNA localized to the vegetal cortex of Xenopus oocytes encodes a protein with a nanos-like zinc finger domain.
1993,
Pubmed Murata,
Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos.
1995,
Pubmed Oulhen,
Retention of exogenous mRNAs selectively in the germ cells of the sea urchin requires only a 5'-cap and a 3'-UTR.
2013,
Pubmed
,
Echinobase Oulhen,
Every which way--nanos gene regulation in echinoderms.
2014,
Pubmed
,
Echinobase Oulhen,
Dysferlin is essential for endocytosis in the sea star oocyte.
2014,
Pubmed
,
Echinobase Oulhen,
The 3'UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin.
2013,
Pubmed
,
Echinobase Saga,
Function of Nanos2 in the male germ cell lineage in mice.
2010,
Pubmed Saito,
Visualization of primordial germ cells in vivo using GFP-nos1 3'UTR mRNA.
2006,
Pubmed Sato,
Maternal Nanos represses hid/skl-dependent apoptosis to maintain the germ line in Drosophila embryos.
2007,
Pubmed Seydoux,
Pathway to totipotency: lessons from germ cells.
2006,
Pubmed Shiio,
Histone sumoylation is associated with transcriptional repression.
2003,
Pubmed Sonoda,
Recruitment of Nanos to hunchback mRNA by Pumilio.
1999,
Pubmed Srikumar,
Global analysis of SUMO chain function reveals multiple roles in chromatin regulation.
2013,
Pubmed Subramaniam,
nos-1 and nos-2, two genes related to Drosophila nanos, regulate primordial germ cell development and survival in Caenorhabditis elegans.
1999,
Pubmed Suzuki,
The Nanos3-3'UTR is required for germ cell specific NANOS3 expression in mouse embryos.
2010,
Pubmed Swartz,
Deadenylase depletion protects inherited mRNAs in primordial germ cells.
2014,
Pubmed
,
Echinobase Tsuda,
Conserved role of nanos proteins in germ cell development.
2003,
Pubmed Voronina,
Vasa protein expression is restricted to the small micromeres of the sea urchin, but is inducible in other lineages early in development.
2008,
Pubmed
,
Echinobase Wang,
Crystal structure of a Pumilio homology domain.
2001,
Pubmed Wang,
nanos function is essential for development and regeneration of planarian germ cells.
2007,
Pubmed Wessel,
Use of sea stars to study basic reproductive processes.
2010,
Pubmed
,
Echinobase Wessel,
The biology of the germ line in echinoderms.
2014,
Pubmed
,
Echinobase Wharton,
RNA regulatory elements mediate control of Drosophila body pattern by the posterior morphogen nanos.
1991,
Pubmed Wreden,
Nanos and pumilio establish embryonic polarity in Drosophila by promoting posterior deadenylation of hunchback mRNA.
1997,
Pubmed Wu,
Cancer. Germ cell genes and cancer.
2010,
Pubmed Wu,
A NANOS3 mutation linked to protein degradation causes premature ovarian insufficiency.
2013,
Pubmed Yajima,
Small micromeres contribute to the germline in the sea urchin.
2011,
Pubmed
,
Echinobase Yajima,
Autonomy in specification of primordial germ cells and their passive translocation in the sea urchin.
2012,
Pubmed
,
Echinobase Zhang,
A conserved RNA-binding protein that regulates sexual fates in the C. elegans hermaphrodite germ line.
1997,
Pubmed
