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The objective of this study is to characterize arterial tissue with and without atherosclerosis by fluorescence lifetime imaging microscopy (FLIM) using Europium Chlortetracycline complex (EuCTc) as fluorescent marker. For this study, twelve rabbits were randomly divided into a control group (CG) and an experimental group (EG), where they were fed a normal and hypercholesterolemic diet, respectively, and were treated for 60 days. Cryosections of the aortic arch specimens were cut in a vertical plane, mounted on glass slides, and stained with Europium (Eu), Chlortetracycline (CTc), Europium Chlortetracycline (EuCTc), and Europium Chlortetracycline Magnesium (EuCTcMg) solutions. FLIM images were obtained with excitation at 405 nm. The average autofluorescence lifetime within plaque depositions was ~1.36 ns. Reduced plaque autofluorescence lifetimes of 0.23 and 0.31 ns were observed on incubation with EuCTc and EuCTcMg respectively. It was observed a quenching of collagen, cholesterol and TG emission spectra increasing EuCTc concentration. The drastic reduction in fluorescence lifetimes is due to a resonant energy transfer between collagen, triglycerides, cholesterol and europium complexes, quenching fluorescence.
Aghayev,
Recent developments in the use of computed tomography scanners in coronary artery imaging.
2016, Pubmed
Aghayev,
Recent developments in the use of computed tomography scanners in coronary artery imaging.
2016,
Pubmed
Andersson-Engels,
Fluorescence imaging and point measurements of tissue: applications to the demarcation of malignant tumors and atherosclerotic lesions from normal tissue.
1991,
Pubmed
Arakawa,
Fluorescence analysis of biochemical constituents identifies atherosclerotic plaque with a thin fibrous cap.
2002,
Pubmed
Ashjian,
Noninvasive in situ evaluation of osteogenic differentiation by time-resolved laser-induced fluorescence spectroscopy.
2004,
Pubmed
Bartorelli,
In vivo human atherosclerotic plaque recognition by laser-excited fluorescence spectroscopy.
1991,
Pubmed
Benet,
Thermodynamics of chelation by tetracyclines.
1966,
Pubmed
Blankstein,
Cardiology patient page. Introduction to noninvasive cardiac imaging.
2012,
Pubmed
Castelli,
Lipids, risk factors and ischaemic heart disease.
1996,
Pubmed
Chou,
Förster Resonance Energy Transfer between Quantum Dot Donors and Quantum Dot Acceptors.
2015,
Pubmed
Coibion,
Binding of the alkali metal cations to tetracycline.
1979,
Pubmed
Di Lullo,
Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human, type I collagen.
2002,
Pubmed
Dunn,
Choice of imaging modality in the assessment of coronary artery disease risk in extreme obesity.
2010,
Pubmed
Dürckheimer,
Tetracyclines: chemistry, biochemistry, and structure-activity relations.
1975,
Pubmed
Escolar,
New imaging techniques for diagnosing coronary artery disease.
2006,
Pubmed
Golub,
Clinical applications of non-antibacterial tetracyclines. Part II.
2011,
Pubmed
Hiltunen,
Expression of LDL receptor, VLDL receptor, LDL receptor-related protein, and scavenger receptor in rabbit atherosclerotic lesions: marked induction of scavenger receptor and VLDL receptor expression during lesion development.
1998,
Pubmed
Jo,
Laguerre-based method for analysis of time-resolved fluorescence data: application to in-vivo characterization and diagnosis of atherosclerotic lesions.
2006,
Pubmed
Keijzer,
Fluorescence spectroscopy of turbid media: Autofluorescence of the human aorta.
1989,
Pubmed
Lindgren,
Accumulation of tetracyclines in atherosclerotic lesions of human aorta.
1966,
Pubmed
Maarek,
Time-resolved fluorescence of human aortic wall: use for improved identification of atherosclerotic lesions.
2000,
Pubmed
Mahía-Casado,
Update on cardiac imaging techniques 2014.
2015,
Pubmed
Marcu,
Discrimination of human coronary artery atherosclerotic lipid-rich lesions by time-resolved laser-induced fluorescence spectroscopy.
2001,
Pubmed
Marcu,
In vivo detection of macrophages in a rabbit atherosclerotic model by time-resolved laser-induced fluorescence spectroscopy.
2005,
Pubmed
Megens,
Optical imaging innovations for atherosclerosis research: multiphoton microscopy and optical nanoscopy.
2015,
Pubmed
Morguet,
Autofluorescence spectroscopy using a XeCl excimer laser system for simultaneous plaque ablation and fluorescence excitation.
1994,
Pubmed
Murata,
Collagen types in various layers of the human aorta and their changes with the atherosclerotic process.
1986,
Pubmed
Nascimento da Silva,
Liquid biopsy of atherosclerosis using protoporphyrin IX as a biomarker.
2014,
Pubmed
,
Echinobase
Phipps,
Fluorescence lifetime imaging for the characterization of the biochemical composition of atherosclerotic plaques.
2011,
Pubmed
Ross,
Atherosclerosis and cancer: common molecular pathways of disease development and progression.
2001,
Pubmed
Ross,
Atherosclerosis--an inflammatory disease.
1999,
Pubmed
Sanz,
Imaging of atherosclerotic cardiovascular disease.
2008,
Pubmed
Sarikaya,
Cardiac applications of PET.
2015,
Pubmed
Sun,
Dynamic tissue analysis using time- and wavelength-resolved fluorescence spectroscopy for atherosclerosis diagnosis.
2011,
Pubmed
Teixeira,
Enhancement on the Europium emission band of Europium chlortetracycline complex in the presence of LDL.
2010,
Pubmed
Vijayalaxmi,
Magnetic resonance imaging (MRI): A review of genetic damage investigations.
2015,
Pubmed