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The first description of complete invertebrate arginine metabolism pathways implies dose-dependent pathogen regulation in Apostichopus japonicus.
Yina S
,
Chenghua L
,
Weiwei Z
,
Zhenhui W
,
Zhimeng L
.
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In this study, three typical members representative of different arginine metabolic pathways were firstly identified from Apostichopus japonicus, including nitric oxide synthase (NOS), arginase, and agmatinase. Spatial expression analysis revealed that the AjNOS transcript presented negative expression patterns relative to those of Ajarginase or Ajagmatinase in most detected tissues. Furthermore, Vibrio splendidus-challenged coelomocytes and intestine, and LPS-exposed primary coelomocytes could significantly induce AjNOS expression, followed by obviously inhibited Arginase and AjAgmatinase transcripts at the most detected time points. Silencing the three members with two specific siRNAs in vivo and in vitro collectively indicated that AjNOS not only compete with Ajarginase but also with Ajagmatinase in arginine metabolism. Interestingly, Ajarginase and Ajagmatinase displayed cooperative expression profiles in arginine utilization. More importantly, live pathogens of V. splendidus and Vibrio parahaemolyticus co-incubated with primary cells also induced NO production and suppressed arginase activity in a time-dependent at an appropriate multiplicity of infection (MOI) of 10, without non-pathogen Escherichia coli. When increasing the pathogen dose (MOI = 100), arginase activity was significantly elevated, and NO production was depressed, with a larger magnitude in V. splendidus co-incubation. The present study expands our understanding of the connection between arginine''s metabolic and immune responses in non-model invertebrates.
Figure 1. The tissue distribution of AjNOS, Ajarginase and Ajagmatinase in normal sea cucumber detected by quantitative PCR.The transcript levels in coelomocytes, intestine, tentacle, and respiratory tress were normalized to that in muscle. Five biological replicates were performed in the experiment and the obtained data were expressed as the mean ± SD (n = 5). Asterisks indicated significant differences: *P < 0.05, **P < 0.01.
Figure 4. Related data from Apostichopus japonicus primary cultured coelomocytes after each gene silencing.(A,D,G) Silencing efficiency of AjNOS, Ajarginase or Ajagmatinase in primary coelomocytes after specific siRNAs transfection and relative expression of mRNAs after interfering for 24 h, respectively. (B,E,H) NO production in the primary cultured coelomocytes after AjNOS, Ajarginase or Ajagmatinase knock-down, respectively. (C,F,I) arginase activity in the primary cultured coelomocytes after AjNOS, Ajarginase or Ajagmatinase knock-down, respectively. Five biological replicates were performed in the experiment and the obtained data were expressed as the mean ± SD (n = 5).
Figure 5. Related data from Apostichopus japonicus individual levels after each gene silencing.(A,D,G) Silencing efficiency of AjNOS, Ajarginase or Ajagmatinase in individuals coelomocytes after specific siRNAs transfection and relative expression of mRNAs after interfering for 24 h, respectively. (B,E,H) NO production in the individuals coelomocytes after AjNOS, Ajarginase or Ajagmatinase knock-down, respectively. (C,F,I) arginase activity in the individuals coelomocytes after AjNOS, Ajarginase or Ajagmatinase knock-down, respectively. Five biological replicates were performed in the experiment and the obtained data were expressed as the mean ± SD (n = 5).
Figure 6. Effects of in vitro stimulation of pathogen or non-pathogen on Apostichopus japonicus primary cultured coelomocytes for 12 and 24 h with an appropriate multiplicity of infection (MOI) of 10 and 100.(A) NO production in the primary cultured coelomocytes after Vibrio splendidus, Vibrio Parahaemolyticus and Escherichia Coli infection. (B) arginase activity in the primary cultured coelomocytes after Vibrio splendidus, Vibrio Parahaemolyticus and Escherichia Coli infection. Five biological replicates were performed in the experiment and the obtained data were expressed as the mean ± SD (n = 5).
Figure 7. Schematic representation of the arginine metabolic pathways of host-pathogen interaction.CAT was short from cationic amino acid transporter and mediated arginine transport. The extracellular urea released by pathogens (Vsagmatinase or Vparginase). Ajarginase and Ajagmatinase transcribed and translated and generated into urea. AjNOS transcribed and translated and generated into NO.
Abu-Soud,
Nitric oxide synthases reveal a role for calmodulin in controlling electron transfer.
1993, Pubmed
Abu-Soud,
Nitric oxide synthases reveal a role for calmodulin in controlling electron transfer.
1993,
Pubmed
Ahn,
Crystal structure of agmatinase reveals structural conservation and inhibition mechanism of the ureohydrolase superfamily.
2004,
Pubmed
Alderton,
Nitric oxide synthases: structure, function and inhibition.
2001,
Pubmed
Andreakis,
Evolution of the nitric oxide synthase family in metazoans.
2011,
Pubmed
Auguet,
Selective inhibition of inducible nitric oxide synthase by agmatine.
1995,
Pubmed
Bansal,
Arginine availability, arginase, and the immune response.
2003,
Pubmed
Berkowitz,
Arginase reciprocally regulates nitric oxide synthase activity and contributes to endothelial dysfunction in aging blood vessels.
2003,
Pubmed
Bogdan,
The multiplex function of nitric oxide in (auto)immunity.
1998,
Pubmed
Bogdan,
Nitric oxide and the immune response.
2001,
Pubmed
Borlace,
A role for altered phagosome maturation in the long-term persistence of Helicobacter pylori infection.
2012,
Pubmed
Buga,
Arginase activity in endothelial cells: inhibition by NG-hydroxy-L-arginine during high-output NO production.
1996,
Pubmed
Bussière,
Spermine causes loss of innate immune response to Helicobacter pylori by inhibition of inducible nitric-oxide synthase translation.
2005,
Pubmed
Chang,
Macrophage arginase promotes tumor cell growth and suppresses nitric oxide-mediated tumor cytotoxicity.
2001,
Pubmed
Chang,
Arginase modulates nitric oxide production in activated macrophages.
1998,
Pubmed
Chen,
Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei.
2015,
Pubmed
Cho,
Calmodulin is a subunit of nitric oxide synthase from macrophages.
1992,
Pubmed
Corraliza,
Determination of arginase activity in macrophages: a micromethod.
1994,
Pubmed
Daghigh,
Inhibition of rat liver arginase by an intermediate in NO biosynthesis, NG-hydroxy-L-arginine: implications for the regulation of nitric oxide biosynthesis by arginase.
1994,
Pubmed
Das,
Modulation of the arginase pathway in the context of microbial pathogenesis: a metabolic enzyme moonlighting as an immune modulator.
2010,
Pubmed
Demady,
Agmatine enhances the NADPH oxidase activity of neuronal NO synthase and leads to oxidative inactivation of the enzyme.
2001,
Pubmed
Fang,
Perspectives series: host/pathogen interactions. Mechanisms of nitric oxide-related antimicrobial activity.
1997,
Pubmed
Galea,
Inhibition of mammalian nitric oxide synthases by agmatine, an endogenous polyamine formed by decarboxylation of arginine.
1996,
Pubmed
Geller,
Molecular biology of nitric oxide synthases.
1998,
Pubmed
Ghosh,
Arginine-induced germ tube formation in Candida albicans is essential for escape from murine macrophage line RAW 264.7.
2009,
Pubmed
Gobert,
L-Arginine availability modulates local nitric oxide production and parasite killing in experimental trypanosomiasis.
2000,
Pubmed
Goda,
The first archaeal agmatinase from anaerobic hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization.
2005,
Pubmed
Griffith,
Nitric oxide synthases: properties and catalytic mechanism.
1995,
Pubmed
Hesse,
Differential regulation of nitric oxide synthase-2 and arginase-1 by type 1/type 2 cytokines in vivo: granulomatous pathology is shaped by the pattern of L-arginine metabolism.
2001,
Pubmed
Hibbs,
Nitric oxide: a cytotoxic activated macrophage effector molecule.
1988,
Pubmed
Iniesta,
Arginase I induction in macrophages, triggered by Th2-type cytokines, supports the growth of intracellular Leishmania parasites.
2002,
Pubmed
Iyer,
Cloning and characterization of human agmatinase.
2002,
Pubmed
Jiang,
Characterization of antimicrobial resistance of Vibrio parahaemolyticus from cultured sea cucumbers (Apostichopus japonicas).
2014,
Pubmed
,
Echinobase
Jiang,
The immunomodulation of inducible nitric oxide in scallop Chlamys farreri.
2013,
Pubmed
Jiang,
Mutual modulation between norepinephrine and nitric oxide in haemocytes during the mollusc immune response.
2014,
Pubmed
Joerink,
Evolutionary conservation of alternative activation of macrophages: structural and functional characterization of arginase 1 and 2 in carp (Cyprinus carpio L.).
2006,
Pubmed
Kim,
Arginase inhibition restores NOS coupling and reverses endothelial dysfunction and vascular stiffness in old rats.
2009,
Pubmed
King,
Arginine in asthma and lung inflammation.
2004,
Pubmed
Li,
Amino acids and immune function.
2007,
Pubmed
Lim,
Mitochondrial arginase II constrains endothelial NOS-3 activity.
2007,
Pubmed
Liu,
Identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers Apostichopus japonicus (Selenka).
2010,
Pubmed
,
Echinobase
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Lorin,
Arginine and nitric oxide synthase: regulatory mechanisms and cardiovascular aspects.
2014,
Pubmed
Losada,
Quantitative and qualitative evaluation of iNOS expression in turbot (Psetta maxima) infected with Enteromyxum scophthalmi.
2012,
Pubmed
Moncada,
Nitric oxide: physiology, pathophysiology, and pharmacology.
1991,
Pubmed
Moncada,
Biosynthesis of nitric oxide from L-arginine. A pathway for the regulation of cell function and communication.
1989,
Pubmed
Morris,
Recent advances in arginine metabolism: roles and regulation of the arginases.
2009,
Pubmed
Morris,
Arginine metabolism in vascular biology and disease.
2005,
Pubmed
Nakane,
Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle.
1993,
Pubmed
Nieves,
Arginine and immunity: a unique perspective.
2002,
Pubmed
Ouzounis,
On the evolution of arginases and related enzymes.
1994,
Pubmed
Perozich,
Roles of conserved residues in the arginase family.
1998,
Pubmed
Popovic,
Arginine and immunity.
2007,
Pubmed
Regulski,
Molecular and biochemical characterization of dNOS: a Drosophila Ca2+/calmodulin-dependent nitric oxide synthase.
1995,
Pubmed
Satriano,
Agmatine suppresses proliferation by frameshift induction of antizyme and attenuation of cellular polyamine levels.
1998,
Pubmed
Sekowska,
Phylogeny of related functions: the case of polyamine biosynthetic enzymes.
2000,
Pubmed
Shao,
Divergent metabolic responses of Apostichopus japonicus suffered from skin ulceration syndrome and pathogen challenge.
2013,
Pubmed
,
Echinobase
Thomas,
The chemical biology of nitric oxide: implications in cellular signaling.
2008,
Pubmed
Tripathi,
Nitric oxide and immune response.
2007,
Pubmed
Vincendeau,
Arginases in parasitic diseases.
2003,
Pubmed
Wanasen,
L-arginine metabolism and its impact on host immunity against Leishmania infection.
2008,
Pubmed
Wang,
Arginine decarboxylase and agmatinase: an alternative pathway for de novo biosynthesis of polyamines for development of mammalian conceptuses.
2014,
Pubmed
Wang,
Molecular cloning, gene organization and expression of rainbow trout (Oncorhynchus mykiss) inducible nitric oxide synthase (iNOS) gene.
2001,
Pubmed
Weiske,
Stimulation of NO synthase activity in the immune-competent lepidopteran Estigmene acraea hemocyte line.
1999,
Pubmed
Wiesinger,
Arginine metabolism and the synthesis of nitric oxide in the nervous system.
2001,
Pubmed
Wijnands,
Arginase-1 deficiency regulates arginine concentrations and NOS2-mediated NO production during endotoxemia.
2014,
Pubmed
Yao,
Molecular cloning and expression of NOS in shrimp, Litopenaeus vannamei.
2010,
Pubmed
Yao,
Expression of nitric oxide synthase (NOS) genes in channel catfish is highly regulated and time dependent after bacterial challenges.
2014,
Pubmed
Yu,
Expression of arginase isozymes in mouse brain.
2001,
Pubmed
Zhang,
Constitutive expression of arginase in microvascular endothelial cells counteracts nitric oxide-mediated vasodilatory function.
2001,
Pubmed
Zhang,
Cloning and characterization of allograft inflammatory factor-1 (AIF-1) from Manila clam Venerupis philippinarum.
2011,
Pubmed
Zhang,
De novo assembly of the sea cucumber Apostichopus japonicus hemocytes transcriptome to identify miRNA targets associated with skin ulceration syndrome.
2013,
Pubmed
,
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