Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Nature
2016 Mar 03;5317592:59-63. doi: 10.1038/nature17182.
Show Gene links
Show Anatomy links
Priming and polymerization of a bacterial contractile tail structure.
Zoued A
,
Durand E
,
Brunet YR
,
Spinelli S
,
Douzi B
,
Guzzo M
,
Flaugnatti N
,
Legrand P
,
Journet L
,
Fronzes R
,
Mignot T
,
Cambillau C
,
Cascales E
.
???displayArticle.abstract???
Contractile tails are composed of an inner tube wrapped by an outer sheath assembled in an extended, metastable conformation that stores mechanical energy necessary for its contraction. Contraction is used to propel the rigid inner tube towards target cells for DNA or toxin delivery. Although recent studies have revealed the structure of the contractile sheath of the type VI secretion system, the mechanisms by which its polymerization is controlled and coordinated with the assembly of the inner tube remain unknown. Here we show that the starfish-like TssA dodecameric complex interacts with tube and sheath components. Fluorescence microscopy experiments in enteroaggregative Escherichia coli reveal that TssA binds first to the type VI secretion system membrane core complex and then initiates tail polymerization. TssA remains at the tip of the growing structure and incorporates new tube and sheath blocks. On the basis of these results, we propose that TssA primes and coordinates tail tube and sheath biogenesis.
Aschtgen,
The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wall.
2010, Pubmed
Aschtgen,
The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wall.
2010,
Pubmed
Basler,
Type VI secretion requires a dynamic contractile phage tail-like structure.
2012,
Pubmed
Basler,
Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions.
2013,
Pubmed
Battesti,
The bacterial two-hybrid system based on adenylate cyclase reconstitution in Escherichia coli.
2012,
Pubmed
Blanc,
Refinement of severely incomplete structures with maximum likelihood in BUSTER-TNT.
2004,
Pubmed
Brunet,
Imaging type VI secretion-mediated bacterial killing.
2013,
Pubmed
Brunet,
Type VI secretion and bacteriophage tail tubes share a common assembly pathway.
2014,
Pubmed
Brunet,
The Type VI Secretion TssEFGK-VgrG Phage-Like Baseplate Is Recruited to the TssJLM Membrane Complex via Multiple Contacts and Serves As Assembly Platform for Tail Tube/Sheath Polymerization.
2015,
Pubmed
Brunet,
An epigenetic switch involving overlapping fur and DNA methylation optimizes expression of a type VI secretion gene cluster.
2011,
Pubmed
Bönemann,
Tubules and donuts: a type VI secretion story.
2010,
Pubmed
Chaveroche,
A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans.
2000,
Pubmed
Chen,
High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy.
2013,
Pubmed
Datsenko,
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
2000,
Pubmed
Douzi,
Crystal structure and self-interaction of the type VI secretion tail-tube protein from enteroaggregative Escherichia coli.
2014,
Pubmed
Durand,
Biogenesis and structure of a type VI secretion membrane core complex.
2015,
Pubmed
Emsley,
Coot: model-building tools for molecular graphics.
2004,
Pubmed
English,
Biochemical analysis of TssK, a core component of the bacterial Type VI secretion system, reveals distinct oligomeric states of TssK and identifies a TssK-TssFG subcomplex.
2014,
Pubmed
Felisberto-Rodrigues,
Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar.
2011,
Pubmed
Ferguson,
Pulse-chase analysis of the in vivo assembly of the bacteriophage T4 tail.
2000,
Pubmed
Fokine,
The molecular architecture of the bacteriophage T4 neck.
2013,
Pubmed
Franke,
DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering.
2009,
Pubmed
Ge,
Atomic structures of a bactericidal contractile nanotube in its pre- and postcontraction states.
2015,
Pubmed
Gerc,
Visualization of the Serratia Type VI Secretion System Reveals Unprovoked Attacks and Dynamic Assembly.
2015,
Pubmed
Heymann,
Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila.
2013,
Pubmed
Kabsch,
XDS.
2010,
Pubmed
Kapitein,
ClpV recycles VipA/VipB tubules and prevents non-productive tubule formation to ensure efficient type VI protein secretion.
2013,
Pubmed
Karimova,
A bacterial two-hybrid system based on a reconstituted signal transduction pathway.
1998,
Pubmed
King,
Assembly of the tail of bacteriophage T4.
1968,
Pubmed
Kube,
Structure of the VipA/B type VI secretion complex suggests a contraction-state-specific recycling mechanism.
2014,
Pubmed
Kudryashev,
Structure of the type VI secretion system contractile sheath.
2015,
Pubmed
Leiman,
Morphogenesis of the T4 tail and tail fibers.
2010,
Pubmed
Leiman,
Contractile tail machines of bacteriophages.
2012,
Pubmed
Leiman,
Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin.
2009,
Pubmed
Pell,
The X-ray crystal structure of the phage lambda tail terminator protein reveals the biologically relevant hexameric ring structure and demonstrates a conserved mechanism of tail termination among diverse long-tailed phages.
2009,
Pubmed
Pettersen,
UCSF Chimera--a visualization system for exploratory research and analysis.
2004,
Pubmed
Rybakova,
Role of antifeeding prophage (Afp) protein Afp16 in terminating the length of the Afp tailocin and stabilizing its sheath.
2013,
Pubmed
Scheres,
RELION: implementation of a Bayesian approach to cryo-EM structure determination.
2012,
Pubmed
Scheres,
Semi-automated selection of cryo-EM particles in RELION-1.3.
2015,
Pubmed
Schindelin,
Fiji: an open-source platform for biological-image analysis.
2012,
Pubmed
Schneider,
Substructure solution with SHELXD.
2002,
Pubmed
Shikuma,
Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures.
2014,
Pubmed
Tang,
EMAN2: an extensible image processing suite for electron microscopy.
2007,
Pubmed
Vegge,
Structural characterization and assembly of the distal tail structure of the temperate lactococcal bacteriophage TP901-1.
2005,
Pubmed
Yang,
Photorhabdus virulence cassettes confer injectable insecticidal activity against the wax moth.
2006,
Pubmed
Yonekura,
The bacterial flagellar cap as the rotary promoter of flagellin self-assembly.
2000,
Pubmed
Zoued,
Architecture and assembly of the Type VI secretion system.
2014,
Pubmed
Zoued,
TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the type VI secretion system.
2013,
Pubmed
van den Ent,
RF cloning: a restriction-free method for inserting target genes into plasmids.
2006,
Pubmed